{"id":405,"date":"2025-06-30T09:42:44","date_gmt":"2025-06-30T09:42:44","guid":{"rendered":"https:\/\/birdstesting.com\/?post_type=product&#038;p=405"},"modified":"2025-06-30T09:42:45","modified_gmt":"2025-06-30T09:42:45","slug":"adenovirus-de-palomas-piadv-dna-test-kit-pcr-ung-50t-100t","status":"publish","type":"product","link":"https:\/\/birdstesting.com\/es\/adenovirus-de-palomas-piadv-dna-test-kit-pcr-ung-50t-100t\/","title":{"rendered":"Kit de prueba de ADN del adenovirus de las palomas (PiADV) - PCR (UNG) 50T\/100T"},"content":{"rendered":"<p data-start=\"270\" data-end=\"431\"><strong data-start=\"270\" data-end=\"349\">Adenovirus de las palomas (PiADV) <a href=\"https:\/\/birdstesting.com\/es\/venta-de-kits-de-analisis-de-adn-de-aves\/\" data-internallinksmanager029f6b8e52c=\"2\" title=\"kit\">Prueba de ADN<\/a> Kit - M\u00e9todo de la sonda fluorescente PCR (UNG)<\/strong><br data-start=\"349\" data-end=\"352\" \/><strong data-start=\"352\" data-end=\"368\">N\u00ba de cat\u00e1logo<\/strong> AP2409005<strong data-start=\"380\" data-end=\"398\">Especificaci\u00f3n:<\/strong> 50T \/ 100T<strong data-start=\"411\" data-end=\"423\">Almacenamiento:<\/strong> -20\u00b0C<\/p>\n<hr data-start=\"433\" data-end=\"436\" \/>\n<h3 data-start=\"438\" data-end=\"465\"><strong data-start=\"442\" data-end=\"463\">Resumen del producto:<\/strong><\/h3>\n<p data-start=\"466\" data-end=\"836\">En <strong data-start=\"470\" data-end=\"512\">Kit de prueba de ADN del adenovirus de las palomas (PiADV)<\/strong> es una herramienta de diagn\u00f3stico qPCR sensible y espec\u00edfica dise\u00f1ada para la detecci\u00f3n de <strong data-start=\"592\" data-end=\"601\">PiADV<\/strong>El PiADV es un virus altamente contagioso que infecta a palomas, loros y otras aves. El PiADV es una de las principales causas de <strong data-start=\"703\" data-end=\"738\">diarrea aguda en palomas j\u00f3venes<\/strong> y se extiende r\u00e1pidamente por <strong data-start=\"772\" data-end=\"808\">transmisi\u00f3n vertical (a trav\u00e9s de los huevos)<\/strong> y <strong data-start=\"813\" data-end=\"835\">contacto horizontal<\/strong>.<\/p>\n<p data-start=\"838\" data-end=\"1080\">Actualmente no existe ning\u00fan tratamiento antiviral eficaz. Por lo tanto, la detecci\u00f3n precoz y precisa mediante este kit de detecci\u00f3n de \u00e1cido nucleico de PiADV es crucial para la gesti\u00f3n oportuna de la enfermedad y para reducir la propagaci\u00f3n viral dentro de las poblaciones de aves.<\/p>\n<p data-start=\"1082\" data-end=\"1295\">Este kit utiliza cebadores optimizados y sondas fluorescentes dirigidas a secuencias g\u00e9nicas conservadas del PiADV. Permite realizar pruebas qPCR precisas y en tiempo real a partir de <strong data-start=\"1231\" data-end=\"1253\">hisopos orales\/cloacales<\/strong>, <strong data-start=\"1255\" data-end=\"1270\">sangre entera<\/strong>y <strong data-start=\"1276\" data-end=\"1294\">muestras de tejido<\/strong>.<\/p>\n<hr data-start=\"1297\" data-end=\"1300\" \/>\n<h3 data-start=\"1302\" data-end=\"1323\"><strong data-start=\"1306\" data-end=\"1323\">Caracter\u00edsticas principales:<\/strong><\/h3>\n<ul data-start=\"1324\" data-end=\"1795\">\n<li data-start=\"1324\" data-end=\"1391\">\n<p data-start=\"1326\" data-end=\"1391\">\u2705 Detecci\u00f3n r\u00e1pida y fiable de <strong data-start=\"1360\" data-end=\"1389\">Adenovirus de las palomas (PiADV)<\/strong><\/p>\n<\/li>\n<li data-start=\"1392\" data-end=\"1470\">\n<p data-start=\"1394\" data-end=\"1470\">\u2705 Aplicable a hisopos orales, hisopos cloacales, sangre (EDTA) y tejidos de \u00f3rganos.<\/p>\n<\/li>\n<li data-start=\"1471\" data-end=\"1546\">\n<p data-start=\"1473\" data-end=\"1546\">Validado para <strong data-start=\"1489\" data-end=\"1526\">PCR cuantitativa en tiempo real (qPCR)<\/strong> utilizando el canal FAM<\/p>\n<\/li>\n<li data-start=\"1547\" data-end=\"1605\">\n<p data-start=\"1549\" data-end=\"1605\">\u2705 Interior <strong data-start=\"1560\" data-end=\"1603\">se incluyeron controles positivos y negativos<\/strong><\/p>\n<\/li>\n<li data-start=\"1606\" data-end=\"1654\">\n<p data-start=\"1608\" data-end=\"1654\">Disponible en <strong data-start=\"1623\" data-end=\"1652\">Kits de 50 y 100 pruebas<\/strong><\/p>\n<\/li>\n<li data-start=\"1655\" data-end=\"1704\">\n<p data-start=\"1657\" data-end=\"1704\">\u2705 Caducidad \u2265 12 meses almacenado a -20\u00b0C<\/p>\n<\/li>\n<li data-start=\"1705\" data-end=\"1795\">\n<p data-start=\"1707\" data-end=\"1795\">\u2705 Ideal para laboratorios de diagn\u00f3stico aviar, veterinarios, criadores y colomb\u00f3filos de competici\u00f3n.<\/p>\n<\/li>\n<\/ul>\n<hr data-start=\"1797\" data-end=\"1800\" \/>\n<h3 data-start=\"1802\" data-end=\"1823\"><strong data-start=\"1806\" data-end=\"1823\">Contenido del kit:<\/strong><\/h3>\n<div class=\"_tableContainer_80l1q_1\">\n<div class=\"_tableWrapper_80l1q_14 group flex w-fit flex-col-reverse\" tabindex=\"-1\">\n<table class=\"w-fit min-w-(--thread-content-width)\" border=\"1\" cellspacing=\"0\" cellpadding=\"2\" data-start=\"1825\" data-end=\"2212\">\n<thead data-start=\"1825\" data-end=\"1902\">\n<tr data-start=\"1825\" data-end=\"1902\">\n<th data-start=\"1825\" data-end=\"1848\" data-col-size=\"sm\">Componente<\/th>\n<th data-start=\"1848\" data-end=\"1874\" data-col-size=\"sm\">50T<\/th>\n<th data-start=\"1874\" data-end=\"1902\" data-col-size=\"sm\">100T<\/th>\n<\/tr>\n<\/thead>\n<tbody data-start=\"1980\" data-end=\"2212\">\n<tr data-start=\"1980\" data-end=\"2056\">\n<td data-start=\"1980\" data-end=\"2002\" data-col-size=\"sm\">Mezcla de reactivos PiADV<\/td>\n<td data-start=\"2002\" data-end=\"2028\" data-col-size=\"sm\">1150 \u00b5L<\/td>\n<td data-start=\"2028\" data-end=\"2056\" data-col-size=\"sm\">2 \u00d7 1150 \u00b5L<\/td>\n<\/tr>\n<tr data-start=\"2057\" data-end=\"2134\">\n<td data-start=\"2057\" data-end=\"2080\" data-col-size=\"sm\">Control positivo<\/td>\n<td data-start=\"2080\" data-end=\"2106\" data-col-size=\"sm\">100 \u00b5L<\/td>\n<td data-start=\"2106\" data-end=\"2134\" data-col-size=\"sm\">100 \u00b5L<\/td>\n<\/tr>\n<tr data-start=\"2135\" data-end=\"2212\">\n<td data-start=\"2135\" data-end=\"2158\" data-col-size=\"sm\">Control negativo<\/td>\n<td data-start=\"2158\" data-end=\"2184\" data-col-size=\"sm\">100 \u00b5L<\/td>\n<td data-start=\"2184\" data-end=\"2212\" data-col-size=\"sm\">100 \u00b5L<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<div class=\"sticky end-(--thread-content-margin) h-0 self-end select-none\">\n<div class=\"absolute end-0 flex items-end\"><\/div>\n<\/div>\n<\/div>\n<\/div>\n<hr data-start=\"2214\" data-end=\"2217\" \/>\n<h3 data-start=\"2219\" data-end=\"2255\"><strong data-start=\"2223\" data-end=\"2255\">Tipos de muestras y requisitos:<\/strong><\/h3>\n<ul data-start=\"2256\" data-end=\"2530\">\n<li data-start=\"2256\" data-end=\"2346\">\n<p data-start=\"2258\" data-end=\"2346\"><strong data-start=\"2258\" data-end=\"2268\">Hisopos:<\/strong> Hisopos est\u00e9riles orales o cloacales, almacenados a 2-8\u00b0C (\u226424h) o -20\u00b0C (si se retrasa).<\/p>\n<\/li>\n<li data-start=\"2347\" data-end=\"2456\">\n<p data-start=\"2349\" data-end=\"2456\"><strong data-start=\"2349\" data-end=\"2359\">Sangre:<\/strong> Sangre total tratada con EDTA (no heparina), fresca o almacenada \u22647 d\u00edas a 2-8\u00b0C o \u22643 meses a -20\u00b0C.<\/p>\n<\/li>\n<li data-start=\"2457\" data-end=\"2530\">\n<p data-start=\"2459\" data-end=\"2530\"><strong data-start=\"2459\" data-end=\"2470\">Tejido:<\/strong> \u2264100 mg de h\u00edgado fresco homogeneizado en 200-500 \u00b5L de agua est\u00e9ril.<\/p>\n<\/li>\n<\/ul>\n<p data-start=\"2532\" data-end=\"2678\">Los \u00e1cidos nucleicos deben extraerse utilizando <strong data-start=\"2572\" data-end=\"2611\">DP202 o kits de extracci\u00f3n equivalentes<\/strong>. El ADN extra\u00eddo puede almacenarse \u22647 d\u00edas a 4\u00b0C o \u22646 meses a -20\u00b0C.<\/p>\n<hr data-start=\"2680\" data-end=\"2683\" \/>\n<h3 data-start=\"2685\" data-end=\"2713\"><strong data-start=\"2689\" data-end=\"2713\">Preparaci\u00f3n de la reacci\u00f3n qPCR:<\/strong><\/h3>\n<ol data-start=\"2714\" data-end=\"3009\">\n<li data-start=\"2714\" data-end=\"2767\">\n<p data-start=\"2717\" data-end=\"2767\">Descongelar completamente los reactivos y centrifugar brevemente.<\/p>\n<\/li>\n<li data-start=\"2768\" data-end=\"3009\">\n<p data-start=\"2771\" data-end=\"2855\">Para cada muestra de ensayo + 2 controles (positivo y negativo), preparar tubos de reacci\u00f3n:<\/p>\n<ul data-start=\"2859\" data-end=\"3009\">\n<li data-start=\"2859\" data-end=\"2896\">\n<p data-start=\"2861\" data-end=\"2896\">A\u00f1adir <strong data-start=\"2865\" data-end=\"2894\">23 \u00b5L de mezcla de detecci\u00f3n PiADV<\/strong><\/p>\n<\/li>\n<li data-start=\"2900\" data-end=\"2969\">\n<p data-start=\"2902\" data-end=\"2969\">A\u00f1adir <strong data-start=\"2906\" data-end=\"2928\">2 \u00b5L de muestra de ADN<\/strong>, <strong data-start=\"2930\" data-end=\"2942\">positivo<\/strong>\u00a0o <strong data-start=\"2947\" data-end=\"2967\">control negativo<\/strong><\/p>\n<\/li>\n<li data-start=\"2973\" data-end=\"3009\">\n<p data-start=\"2975\" data-end=\"3009\">Volumen total: <strong data-start=\"2989\" data-end=\"2998\">25 \u00b5L<\/strong> por tubo<\/p>\n<\/li>\n<\/ul>\n<\/li>\n<\/ol>\n<hr data-start=\"3011\" data-end=\"3014\" \/>\n<h3 data-start=\"3016\" data-end=\"3047\"><strong data-start=\"3020\" data-end=\"3047\">PCR Cycling Parameters:<\/strong><\/h3>\n<div class=\"_tableContainer_80l1q_1\">\n<div class=\"_tableWrapper_80l1q_14 group flex w-fit flex-col-reverse\" tabindex=\"-1\">\n<table class=\"w-fit min-w-(--thread-content-width)\" border=\"1\" cellspacing=\"0\" cellpadding=\"2\" data-start=\"3049\" data-end=\"3372\">\n<thead data-start=\"3049\" data-end=\"3102\">\n<tr data-start=\"3049\" data-end=\"3102\">\n<th data-start=\"3049\" data-end=\"3071\" data-col-size=\"sm\">Paso<\/th>\n<th data-start=\"3071\" data-end=\"3085\" data-col-size=\"sm\">Temperatura<\/th>\n<th data-start=\"3085\" data-end=\"3102\" data-col-size=\"sm\">Tiempo<\/th>\n<\/tr>\n<\/thead>\n<tbody data-start=\"3157\" data-end=\"3372\">\n<tr data-start=\"3157\" data-end=\"3210\">\n<td data-start=\"3157\" data-end=\"3179\" data-col-size=\"sm\">Descontaminaci\u00f3n UNG<\/td>\n<td data-start=\"3179\" data-end=\"3193\" data-col-size=\"sm\">50\u00b0C<\/td>\n<td data-start=\"3193\" data-end=\"3210\" data-col-size=\"sm\">2 minutos<\/td>\n<\/tr>\n<tr data-start=\"3211\" data-end=\"3264\">\n<td data-start=\"3211\" data-end=\"3233\" data-col-size=\"sm\">Pre-desnaturalizaci\u00f3n<\/td>\n<td data-start=\"3233\" data-end=\"3247\" data-col-size=\"sm\">95\u00b0C<\/td>\n<td data-start=\"3247\" data-end=\"3264\" data-col-size=\"sm\">5 minutos<\/td>\n<\/tr>\n<tr data-start=\"3265\" data-end=\"3318\">\n<td data-start=\"3265\" data-end=\"3287\" data-col-size=\"sm\">Amplificaci\u00f3n (\u00d740)<\/td>\n<td data-start=\"3287\" data-end=\"3301\" data-col-size=\"sm\">95\u00b0C<\/td>\n<td data-start=\"3301\" data-end=\"3318\" data-col-size=\"sm\">10 segundos<\/td>\n<\/tr>\n<tr data-start=\"3319\" data-end=\"3372\">\n<td data-start=\"3319\" data-end=\"3341\" data-col-size=\"sm\"><\/td>\n<td data-start=\"3341\" data-end=\"3355\" data-col-size=\"sm\">60\u00b0C<\/td>\n<td data-start=\"3355\" data-end=\"3372\" data-col-size=\"sm\">35 seg (FAM)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<div class=\"sticky end-(--thread-content-margin) h-0 self-end select-none\">\n<div class=\"absolute end-0 flex items-end\"><\/div>\n<\/div>\n<\/div>\n<\/div>\n<blockquote data-start=\"3374\" data-end=\"3458\">\n<p data-start=\"3376\" data-end=\"3458\">Compatible con ABI y otros sistemas de PCR en tiempo real. Colorante ROX opcional si se requiere.<\/p>\n<\/blockquote>\n<hr data-start=\"3460\" data-end=\"3463\" \/>\n<h3 data-start=\"3465\" data-end=\"3495\"><strong data-start=\"3469\" data-end=\"3495\">Interpretaci\u00f3n de los resultados:<\/strong><\/h3>\n<ul data-start=\"3496\" data-end=\"3629\">\n<li data-start=\"3496\" data-end=\"3536\">\n<p data-start=\"3498\" data-end=\"3536\"><strong data-start=\"3498\" data-end=\"3511\">Positivo:<\/strong> Ct \u2264 33 con curva en \"S\".<\/p>\n<\/li>\n<li data-start=\"3537\" data-end=\"3583\">\n<p data-start=\"3539\" data-end=\"3583\"><strong data-start=\"3539\" data-end=\"3551\">Sospechoso:<\/strong> Ct 33-38 - se recomienda repetir la prueba<\/p>\n<\/li>\n<li data-start=\"3584\" data-end=\"3629\">\n<p data-start=\"3586\" data-end=\"3629\"><strong data-start=\"3586\" data-end=\"3599\">Negativo:<\/strong> Ct \u2265 38 o sin amplificaci\u00f3n.<\/p>\n<\/li>\n<\/ul>\n<p data-start=\"3631\" data-end=\"3672\"><strong data-start=\"3631\" data-end=\"3672\">Los controles deben cumplir criterios de validez:<\/strong><\/p>\n<ul data-start=\"3673\" data-end=\"3743\">\n<li data-start=\"3673\" data-end=\"3701\">\n<p data-start=\"3675\" data-end=\"3701\">Control positivo Ct &lt; 28<\/p>\n<\/li>\n<li data-start=\"3702\" data-end=\"3743\">\n<p data-start=\"3704\" data-end=\"3743\">Control negativo: Ct \u2265 38 o no detectado.<\/p>\n<\/li>\n<\/ul>\n<hr data-start=\"3745\" data-end=\"3748\" \/>\n<h3 data-start=\"3750\" data-end=\"3774\"><strong data-start=\"3754\" data-end=\"3774\">Notas importantes:<\/strong><\/h3>\n<ol data-start=\"3775\" data-end=\"4155\">\n<li data-start=\"3775\" data-end=\"3877\">\n<p data-start=\"3778\" data-end=\"3877\">Separaci\u00f3n estricta de las zonas de preparaci\u00f3n de muestras, manipulaci\u00f3n de reactivos y amplificaci\u00f3n para evitar la contaminaci\u00f3n.<\/p>\n<\/li>\n<li data-start=\"3878\" data-end=\"3929\">\n<p data-start=\"3881\" data-end=\"3929\">Llevar guantes y ropa de laboratorio; cambiarse regularmente<\/p>\n<\/li>\n<li data-start=\"3930\" data-end=\"3994\">\n<p data-start=\"3933\" data-end=\"3994\">No abrir los tubos PCR inmediatamente despu\u00e9s de la ejecuci\u00f3n - dejar enfriar<\/p>\n<\/li>\n<li data-start=\"3995\" data-end=\"4038\">\n<p data-start=\"3998\" data-end=\"4038\">Evitar la congelaci\u00f3n-descongelaci\u00f3n repetida de los reactivos<\/p>\n<\/li>\n<li data-start=\"4039\" data-end=\"4100\">\n<p data-start=\"4042\" data-end=\"4100\">S\u00f3lo para uso de investigaci\u00f3n - <strong data-start=\"4066\" data-end=\"4098\">no para diagn\u00f3stico cl\u00ednico<\/strong><\/p>\n<\/li>\n<li data-start=\"4101\" data-end=\"4155\">\n<p data-start=\"4104\" data-end=\"4155\">Proteger los reactivos de la luz y almacenarlos seg\u00fan las instrucciones<\/p>\n<\/li>\n<\/ol>\n<hr data-start=\"4157\" data-end=\"4160\" \/>\n<p data-start=\"4162\" data-end=\"4330\">\ud83d\uded2 <strong data-start=\"4165\" data-end=\"4236\">Detecci\u00f3n segura y fiable del PiADV en sus palomas o especies aviares<\/strong>. Perfecto para la detecci\u00f3n de enfermedades, el control sanitario de instalaciones de cr\u00eda y la investigaci\u00f3n cient\u00edfica.<\/p>","protected":false},"excerpt":{"rendered":"<p>Pigeon Adenovirus (PiADV) DNA Test Kit - PCR Fluorescent Probe Method (UNG)Catalog No.: AP2409005 Specification: 50T \/ 100T Almacenamiento: -20\u00b0C Descripci\u00f3n general del producto: El Pigeon Adenovirus (PiADV) DNA Test Kit es una herramienta de diagn\u00f3stico qPCR sensible y espec\u00edfica dise\u00f1ada para la detecci\u00f3n de PiADV, un virus altamente contagioso que infecta a palomas, loros y otras aves. El PiADV es un...<\/p>","protected":false},"featured_media":406,"comment_status":"open","ping_status":"closed","template":"","meta":{"_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"default","_kad_post_title":"","_kad_post_layout":"default","_kad_post_sidebar_id":"","_kad_post_content_style":"default","_kad_post_vertical_padding":"default","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"_kad_post_classname":""},"product_brand":[],"product_cat":[125],"product_tag":[],"class_list":["post-405","product","type-product","status-publish","has-post-thumbnail","product_cat-bird-dna-test","first","instock","shipping-taxable","product-type-simple","entry","content-bg","loop-entry"],"_links":{"self":[{"href":"https:\/\/birdstesting.com\/es\/wp-json\/wp\/v2\/product\/405","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/birdstesting.com\/es\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/birdstesting.com\/es\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/birdstesting.com\/es\/wp-json\/wp\/v2\/comments?post=405"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/birdstesting.com\/es\/wp-json\/wp\/v2\/media\/406"}],"wp:attachment":[{"href":"https:\/\/birdstesting.com\/es\/wp-json\/wp\/v2\/media?parent=405"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/birdstesting.com\/es\/wp-json\/wp\/v2\/product_brand?post=405"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/birdstesting.com\/es\/wp-json\/wp\/v2\/product_cat?post=405"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/birdstesting.com\/es\/wp-json\/wp\/v2\/product_tag?post=405"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}