Pigeon C. Albicans (PiCA) DNA Test Kit – Real-Time PCR Fluorescent Probe Method (50T/100T)

SKU: AP2409007
Specifications: 50 Tests / 100 Tests
ストレージ： Store at -20°C

Pigeon C. Albicans (PiCA) DNA Test Kit Overview:

について Pigeon Candida albicans (PiCA) DNA Test Kit is a molecular diagnostic tool designed to detect Candida albicans, the fungal pathogen responsible for pigeon thrush (also known as mycotic stomatitis or oral candidiasis). This disease frequently occurs in warm, humid climates and is characterized by the formation of white pseudomembranes and ulcers in the mouth, esophagus, and crop lining of birds, particularly pigeons.

Using a highly sensitive and specific real-time PCR fluorescent probe method, this kit allows for early, accurate detection of PiCA in samples such as oropharyngeal/cloacal swabs, blood, or tissue. This enables bird breeders, avian veterinarians, and pigeon enthusiasts to monitor and control infections effectively, reducing the spread and preventing complications.

Key Features:
✅ High sensitivity and specificity with optimized primers targeting conserved regions of PiCA DNA
✅ Fast and precise quantitative results via qPCR technology
✅ Applicable to oropharyngeal swabs, cloacal swabs, fresh blood (EDTA), and tissue homogenates
✅ Includes positive and negative controls for accurate validation

Kit Components:

• PiCA Detection Reagent: 1150μL × 1 (50T) / ×2 (100T)

• Positive Control: 100μL

• Negative Control: 100μL

• PCR tubes included (0.2mL)

Storage & Shelf Life:

• Store at -20°C

• Stable for at least 12 months

Sample Preparation Guidelines:

• Swabs: Collect from oropharynx or cloaca using sterile swabs. Store at 2–8°C for <24h or at -20°C for longer periods.

• Whole Blood: Use EDTA as an anticoagulant. Store at 2–8°C (up to 7 days) or -20°C (up to 3 months).

• Tissue Samples: Use ≤100mg of fresh liver; homogenize in sterile water.

Test Workflow (Simplified):

1. Extract DNA using a validated kit (e.g., DP202).

2. Prepare PCR mix: 23μL reagent + 2μL sample/control = 25μL total.

3. Run qPCR with the following parameters:

   • 50°C for 2 min (UNG treatment)

   • 95°C for 5 min

   • 40 cycles: 95°C for 10s, 60°C for 35s (FAM channel for detection)

Result Interpretation:

• Ct ≤ 33 with an S-shaped curve: Positive

• Ct 33–38: Suspect – re-test recommended

• Ct ≥ 38 or no curve: Negative

Precautions:

• Maintain separate areas for sample prep, reagent handling, and PCR to avoid contamination.

• Follow all biosafety and disinfection protocols.

• This product is for research use only and not intended for clinical diagnostic purposes.