ハトヘルペスウイルス（PiHV）DNA検査キット-PCR蛍光プローブ法（50T / 100T）

Pigeon Herpesvirus (PiHV) DNA Test Kit

This avian DNA検査 キット is designed to detect Pigeon Herpesvirus Type I (PiHV1), a major cause of young pigeon disease syndrome. PiHV infections typically affect young pigeons and are characterized by rhinitis, conjunctivitis, lethargy, loss of appetite, diarrhea, vomiting, and neurological symptoms.

Using a highly optimized PCR-fluorescent probe method, this kit offers fast and accurate detection of PiHV DNA in bird oral/cloacal swabs, blood, and tissue samples. Early detection helps prevent disease outbreaks, reduce mortality, and support effective treatment strategies.

Key Features:

✅ Detects Pigeon Herpesvirus (PiHV1) with high specificity and sensitivity
✅ Sample compatibility: oral/cloacal swabs, EDTA blood, liver tissue
✅ Includes positive and negative controls for each run
✅ Easy PCR workflow with minimal hands-on time
✅ Ideal for use in pigeon lofts, vet labs, and disease monitoring programs
✅ Shelf life: ≥12 months at -20°C

Suitable For:

• Racing pigeons

• Fancy pigeons

• Young pigeons in breeding programs

• All PiHV-susceptible avian species

Kit Components:

PCR Protocol:

• Reaction volume: 25 μL (23 μL reagent + 2 μL sample)

• Thermal cycling:
  • 50°C for 2 min (UNG)
  • 95°C for 5 min
  • 40 cycles of:
     95°C for 10 sec
     60°C for 35 sec (fluorescence collection)

• Detection Channel: FAM

• Compatible with ABI and most real-time PCR instruments

Result Interpretation:

• Positive: Ct ≤ 33 and S-curve present

• Suspect: 33 < Ct < 38 – retesting recommended after ruling out inhibitors or contamination

• Negative: No Ct or Ct ≥ 38 with no S-curve

Positive and negative controls must meet QC criteria for results to be valid.

Storage & Handling:

• Store at -20°C

• Avoid repeated freeze-thaw cycles

• Protect reagents from light

• For research use only – not for clinical diagnostics

Recommended Use With:

• DNA Extraction Kit (e.g., DP202)

• PPE and aerosol contamination control

• Other bird virus PCR kits (PBFD, Polyomavirus, Chlamydia psittaci, etc.)

• 鳥の性別 test kits for full avian DNA profiling

Protect your pigeons with accurate viral DNA testing.
Add this PiHV DNA test kit to your avian health screening toolbox.

🛒 Order now and ensure early detection of pigeon herpesvirus!