{"id":407,"date":"2025-06-30T10:07:55","date_gmt":"2025-06-30T10:07:55","guid":{"rendered":"https:\/\/birdstesting.com\/?post_type=product&p=407"},"modified":"2025-06-30T10:07:56","modified_gmt":"2025-06-30T10:07:56","slug":"%e9%b3%a5%e3%82%a4%e3%83%b3%e3%83%95%e3%83%ab%e3%82%a8%e3%83%b3%e3%82%b6%e6%a4%9c%e6%9f%bb%e3%82%ad%e3%83%83%e3%83%88","status":"publish","type":"product","link":"https:\/\/birdstesting.com\/ja\/%e9%b3%a5%e3%82%a4%e3%83%b3%e3%83%95%e3%83%ab%e3%82%a8%e3%83%b3%e3%82%b6%e6%a4%9c%e6%9f%bb%e3%82%ad%e3%83%83%e3%83%88\/","title":{"rendered":"\u9ce5\u30a4\u30f3\u30d5\u30eb\u30a8\u30f3\u30b6\u691c\u67fb\u30ad\u30c3\u30c8\uff08H1N1\u4e9c\u578b\u30a6\u30a4\u30eb\u30b9\uff09\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u691c\u51fa\u30ad\u30c3\u30c8"},"content":{"rendered":"

\u88fd\u54c1\u540d\uff08EN\uff09\uff1a <\/strong> \u9ce5\u30a4\u30f3\u30d5\u30eb\u30a8\u30f3\u30b6 \u30c6\u30b9\u30c8\u30ad\u30c3\u30c8<\/a>
\u30d1\u30c3\u30b1\u30fc\u30b8\u30b5\u30a4\u30ba\uff1a<\/strong> 50\u30c6\u30b9\u30c8
\u4fa1\u683c\u3060\uff1a<\/strong> \u55b6\u696d\u90e8\u3078\u306e\u304a\u554f\u3044\u5408\u308f\u305b
\u30b9\u30c8\u30ec\u30fc\u30b8\uff1a<\/strong> \u4f4e\u6e29\u3067\u8f38\u9001\u3059\u308b\u3002 -20\u00b0C<\/strong>, 12\u30f6\u6708\u9593\u6709\u52b9
\u767a\u9001\u5143<\/strong> \u4e2d\u56fd\u3001\u4e0a\u6d77
\u7814\u7a76\u7528<\/strong><\/p>\n

\n

\u9ce5\u30a4\u30f3\u30d5\u30eb\u30a8\u30f3\u30b6\u691c\u67fb\u30ad\u30c3\u30c8\u306e\u6982\u8981<\/strong><\/h2>\n

\u3053\u306e\u9ce5\u30a4\u30f3\u30d5\u30eb\u30a8\u30f3\u30b6\u691c\u67fb\u30ad\u30c3\u30c8\u306f\u3001\u7279\u306b\u4ee5\u4e0b\u306e\u76ee\u7684\u3067\u8a2d\u8a08\u3055\u308c\u3066\u3044\u307e\u3059\u3002 \u30a4\u30f3\u30d5\u30eb\u30a8\u30f3\u30b6A\u30a6\u30a4\u30eb\u30b9H1N1\u4e9c\u578bRNA\u306e\u5b9a\u6027\u7684\u304a\u3088\u3073\u5b9a\u91cf\u7684\u691c\u51fa<\/strong> \u4f7f\u7528\u3057\u3066 \u30d7\u30ed\u30fc\u30d6\u30d9\u30fc\u30b9\u306e\u86cd\u5149qRT-PCR\u6280\u8853<\/strong>. .H1N1\u4e9c\u578b\u306e\u4fdd\u5b58\u3055\u308c\u305f\u907a\u4f1d\u5b50\u9818\u57df\u3092\u6a19\u7684\u3068\u3059\u308b\u3053\u3068\u3067\u3001\u3053\u306e\u30ad\u30c3\u30c8\u306f\u751f\u7269\u5b66\u7684\u304a\u3088\u3073\u74b0\u5883\u7684\u30b5\u30f3\u30d7\u30eb\u304b\u3089\u9ce5\u30a4\u30f3\u30d5\u30eb\u30a8\u30f3\u30b6A\u30a6\u30a4\u30eb\u30b9\u3092\u691c\u51fa\u3059\u308b\u969b\u306b\u9ad8\u3044\u7279\u7570\u6027\u3068\u611f\u5ea6\u3092\u63d0\u4f9b\u3059\u308b\u3002.<\/p>\n

SYBR Green\u8272\u7d20\u3092\u7528\u3044\u305fPCR\u3068\u306f\u7570\u306a\u308a TaqMan\u30d7\u30ed\u30fc\u30d6\u30fb\u30d9\u30fc\u30b9\u30fb\u30b7\u30b9\u30c6\u30e0<\/strong> \u306f\u3001\u30cf\u30a4\u30d6\u30ea\u30c0\u30a4\u30bc\u30fc\u30b7\u30e7\u30f3\u3068\u5897\u5e45\u4e2d\u306e\u5207\u65ad\u6642\u306b\u306e\u307f\u30b7\u30b0\u30ca\u30eb\u3092\u767a\u3059\u308b\u86cd\u5149\u6a19\u8b58\u30d7\u30ed\u30fc\u30d6\u3092\u4f7f\u7528\u3057\u3066\u3044\u308b\u305f\u3081\u3001\u78ba\u5b9f\u3067\u3042\u308b\u3002 \u512a\u308c\u305f\u7279\u7570\u6027<\/strong> \u507d\u967d\u6027\u7387\u304c\u4f4e\u3044\u3002.<\/p>\n

\n

\u4e3b\u306a\u7279\u5fb4<\/strong><\/h2>\n

\u2705 \u9ad8\u3044\u7279\u7570\u6027\u3068\u611f\u53d7\u6027<\/strong> - H1N1\u30b2\u30ce\u30e0\u306e\u9ad8\u5ea6\u306b\u4fdd\u5b58\u3055\u308c\u305f\u9818\u57df\u306b\u57fa\u3065\u3044\u3066\u8a2d\u8a08\u3055\u308c\u3066\u304a\u308a\u3001\u975e\u6a19\u7684\u30a6\u30a4\u30eb\u30b9\u3068\u306e\u4ea4\u5dee\u53cd\u5fdc\u306f\u306a\u3044\u3002
\u2705 \u3059\u3050\u306b\u4f7f\u3048\u308b\u30b7\u30b9\u30c6\u30e0<\/strong> - \u30e6\u30fc\u30b6\u30fc\u304b\u3089\u306eRNA\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u306e\u307f\u304c\u5fc5\u8981
\u2705 \u6700\u9069\u5316\u3055\u308c\u305f\u30d7\u30e9\u30a4\u30de\u30fc\u3068\u30d7\u30ed\u30fc\u30d6<\/strong> - \u52b9\u7387\u7684\u306a\u5897\u5e45\u3068\u691c\u51fa\u3092\u4fdd\u8a3c
\u2705 \u30dd\u30b8\u30c6\u30a3\u30d6\u30fb\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u3092\u542b\u3080<\/strong> - \u7d50\u679c\u691c\u8a3c\u306e\u305f\u3081\u306e\u5408\u6210\u975e\u611f\u67d3\u6027DNA
\u2705 \u5b9a\u91cf\u7684\u30fb\u5b9a\u6027\u7684\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3<\/strong> - \u7814\u7a76\u3001\u30b5\u30fc\u30d9\u30a4\u30e9\u30f3\u30b9\u3001\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u306b\u9069\u3057\u3066\u3044\u308b
\u2705 \u4e3b\u8981\u306aqPCR\u88c5\u7f6e\u306b\u5bfe\u5fdc<\/strong> - FAM\u30c1\u30e3\u30f3\u30cd\u30eb\u3067\u306e\u86cd\u5149\u691c\u51fa<\/p>\n

\n

\u30ad\u30c3\u30c8\u5185\u5bb9\uff0850\u30c6\u30b9\u30c8\uff09<\/strong><\/h2>\n
\n
\n\n\n\n\n\n\n\n\n\n\n\n\n
\u30b3\u30f3\u30dd\u30fc\u30cd\u30f3\u30c8\u306e\u8aac\u660e<\/th>\n\u30b3\u30fc\u30c9<\/th>\n\u5bb9\u91cf\u3068\u30ad\u30e3\u30c3\u30d7\u306e\u8272<\/th>\n<\/tr>\n<\/thead>\n
qRT-PCR\u30d0\u30c3\u30d5\u30a1\u30fc\uff08\u30d7\u30ed\u30fc\u30d6\u7cfb\uff09<\/td>\nA<\/td>\n500 \u00b5L<\/td>\n<\/tr>\n
qRT-PCR\u9175\u7d20\u30df\u30c3\u30af\u30b9<\/td>\nB<\/td>\n100 \u00b5L<\/td>\n<\/tr>\n
PCR\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u5e0c\u91c8\u30d0\u30c3\u30d5\u30a1\u30fc<\/td>\nC<\/td>\n1 mL<\/td>\n<\/tr>\n
H1N1\u7528qRT-PCR\u30d7\u30e9\u30a4\u30de\u30fc\u30df\u30c3\u30af\u30b9<\/td>\nD<\/td>\n100 \u00b5L<\/td>\n<\/tr>\n
H1N1\u4e9c\u578b\u7528\u30d7\u30ed\u30fc\u30d6<\/td>\nE<\/td>\n50 \u00b5L<\/td>\n<\/tr>\n
\u30dd\u30b8\u30c6\u30a3\u30d6\u30b3\u30f3\u30c8\u30ed\u30fc\u30ebDNA\uff081\u00d710\u2078\u30b3\u30d4\u30fc\/\u03bcL\uff09<\/td>\nF<\/td>\n50 \u00b5L<\/td>\n<\/tr>\n
RNA\u653e\u51fa\u8a66\u85ac\uff08\u8a66\u9a13\u3001\u7121\u62bd\u51fa\u6cd5\uff09<\/td>\nG<\/td>\n20\u30c6\u30b9\u30c8<\/td>\n<\/tr>\n
\u30e6\u30fc\u30b6\u30fc\u30de\u30cb\u30e5\u30a2\u30eb<\/td>\nH<\/td>\n1\u679a<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
\n
<\/div>\n<\/div>\n<\/div>\n<\/div>\n
\n

\u4ed5\u7d44\u307f<\/strong><\/h3>\n

\u3053\u306e\u30ad\u30c3\u30c8\u306f\u4e00\u6bb5\u968e\u306e\u9006\u8ee2\u5199PCR\u53cd\u5fdc\u306b\u3088\u3063\u3066\u30a6\u30a4\u30eb\u30b9RNA\u3092\u691c\u51fa\u3059\u308b\u3002\u5897\u5e45\u4e2d Taq\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u304c\u4e8c\u91cd\u6a19\u8b58\u30d7\u30ed\u30fc\u30d6\u3092\u5207\u65ad\u3059\u308b\u3002<\/strong>, \u305d\u306e\u7d50\u679c\u3001\u6a19\u7684RNA\u306e\u91cf\u306b\u6bd4\u4f8b\u3057\u305f\u86cd\u5149\u30b7\u30b0\u30ca\u30eb\u304c\u5f97\u3089\u308c\u308b\u3002.<\/p>\n

\u86cd\u5149\u306f\u5404\u30b5\u30a4\u30af\u30eb\u3067\u6e2c\u5b9a\u3055\u308c\u308b\u3002 \u30ea\u30a2\u30eb\u30bf\u30a4\u30e0\u30e2\u30cb\u30bf\u30ea\u30f3\u30b0<\/strong> PCR\u30d7\u30ed\u30bb\u30b9\u306e.<\/p>\n

\n

\u691c\u51fa\u306e\u539f\u7406<\/strong><\/h2>\n
    \n
  • \n

    Ct\u5024\uff08\u30b5\u30a4\u30af\u30eb\u3057\u304d\u3044\u5024\uff09\uff1a<\/strong> \u86cd\u5149\u304c\u95be\u5024\u3092\u8d85\u3048\u308b\u306e\u306b\u5fc5\u8981\u306aPCR\u30b5\u30a4\u30af\u30eb\u6570\u3002Ct\u304c\u4f4e\u3044\u307b\u3069\u521d\u671f\u6a19\u7684\u91cf\u304c\u591a\u3044\u3053\u3068\u3092\u793a\u3059\u3002.<\/p>\n<\/li>\n

  • \n

    \u3057\u304d\u3044\u5024\u306e\u8a2d\u5b9a\uff1a<\/strong> \u6700\u521d\u306e3\uff5e15\u30b5\u30a4\u30af\u30eb\uff08\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\uff09\u304b\u3089\u306e\u86cd\u5149\u306e10\u00d7\u6a19\u6e96\u504f\u5dee\u306b\u57fa\u3065\u304f\u3002.<\/p>\n<\/li>\n<\/ul>\n

    \n

    \u30d1\u30d5\u30a9\u30fc\u30de\u30f3\u30b9\u3068\u30ab\u30c3\u30c8\u30aa\u30d5\u57fa\u6e96<\/strong><\/h3>\n
    \n
    \n\n\n\n\n\n\n\n
    \u7d50\u679c\u306e\u7a2e\u985e<\/th>\n\u57fa\u6e96<\/th>\n<\/tr>\n<\/thead>\n
    \u30dd\u30b8\u30c6\u30a3\u30d6<\/strong><\/td>\nCt\u226635\u3067\u5178\u578b\u7684\u306aS\u5b57\u578b\u5897\u5e45\u66f2\u7dda\u3092\u793a\u3059\u3002<\/td>\n<\/tr>\n
    \u967d\u6027\u306e\u7591\u3044<\/strong><\/td>\nCt 35-40 \u2192\u518d\u691c\u67fb\u3092\u63a8\u5968<\/td>\n<\/tr>\n
    \u30cd\u30ac\u30c6\u30a3\u30d6<\/strong><\/td>\nCt\u226740\u307e\u305f\u306fCt\u306a\u3057\uff0b\u5897\u5e45\u66f2\u7dda\u306a\u3057<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
    \n
    <\/div>\n<\/div>\n<\/div>\n<\/div>\n

    \u967d\u6027\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u3068\u9670\u6027\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u306f\u54c1\u8cea\u30c1\u30a7\u30c3\u30af\u306b\u5408\u683c\u3057\u306a\u3051\u308c\u3070\u306a\u3089\u306a\u3044\u3002\u6307\u6570\u95a2\u6570\u7684\u5897\u5e45\u3067\u9670\u6027\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u306eCt\u226740\u3001\u967d\u6027\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u306eCt\u226630\u3002.<\/p>\n

    \n

    \u53cd\u5fdc\u30d7\u30ed\u30c8\u30b3\u30eb<\/strong><\/h2>\n

    \u9006\u8ee2\u5199\uff0bPCR\uff081\u53cd\u5fdc\u3042\u305f\u308a20\u03bcL\uff09<\/strong><\/h4>\n
    \n
    \n\n\n\n\n\n\n\n\n\n
    \u8a66\u85ac<\/th>\n\u30dc\u30ea\u30e5\u30fc\u30e0<\/th>\n<\/tr>\n<\/thead>\n
    qRT-PCR\u30d0\u30c3\u30d5\u30a1\u30fc<\/td>\n10 \u03bcL<\/td>\n<\/tr>\n
    \u9175\u7d20\u30df\u30c3\u30af\u30b9<\/td>\n2 \u03bcL<\/td>\n<\/tr>\n
    \u30d7\u30e9\u30a4\u30de\u30fc\u30df\u30c3\u30af\u30b9<\/td>\n2 \u03bcL<\/td>\n<\/tr>\n
    \u30d7\u30ed\u30fc\u30d6<\/td>\n1 \u03bcL<\/td>\n<\/tr>\n
    RNA\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u307e\u305f\u306f\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb<\/td>\n5 \u03bcL<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
    \n
    <\/div>\n<\/div>\n<\/div>\n<\/div>\n
      \n
    • \n

      \u7dcf\u91cf\uff1a<\/strong> 20 \u03bcL<\/p>\n<\/li>\n

    • \n

      \u71b1\u30b5\u30a4\u30af\u30eb\u6761\u4ef6\uff1a<\/strong><\/p>\n<\/li>\n<\/ul>\n

      \n
      \n\n\n\n\n\n\n\n\n\n
      \u30b9\u30c6\u30c3\u30d7<\/th>\n\u6e29\u5ea6<\/th>\n\u6642\u9593<\/th>\n<\/tr>\n<\/thead>\n
      \u9006\u8ee2\u5199<\/td>\n50\u00b0C<\/td>\n30\u5206<\/td>\n<\/tr>\n
      \u521d\u671f\u5909\u6027<\/td>\n94\u00b0C<\/td>\n10\u5206<\/td>\n<\/tr>\n
      PCR\u30b5\u30a4\u30af\u30ea\u30f3\u30b0\u00d740<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
      - \u5909\u6027<\/td>\n94\u00b0C<\/td>\n15\u79d2<\/td>\n<\/tr>\n
      - \u30a2\u30cb\u30fc\u30eb\uff0f\u30a8\u30af\u30b9\u30c6\u30f3\u30b7\u30e7\u30f3<\/td>\n60\u00b0C<\/td>\n1\u5206\uff08FAM\uff09<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
      \n
      <\/div>\n<\/div>\n<\/div>\n<\/div>\n
      \n

      \u5b9a\u91cf\u5316\uff08\u30aa\u30d7\u30b7\u30e7\u30f3\uff09<\/strong><\/h3>\n

      \u3092\u7528\u610f\u3059\u308b\u3002 \u691c\u91cf\u7dda<\/strong> \u5408\u6210DNA\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u306e\u9023\u7d9a10\u500d\u5e0c\u91c8\u6db2\uff0810\u00b2-10\u2077\u30b3\u30d4\u30fc\/\u03bcL\uff09\u3092\u4f7f\u7528\u3002Ct\u5024\u3092log\uff08\u6fc3\u5ea6\uff09\u306b\u5bfe\u3057\u3066\u30d7\u30ed\u30c3\u30c8\u3057\u3001\u672a\u77e5\u30b5\u30f3\u30d7\u30eb\u306eRNA\u30b3\u30d4\u30fc\u6570\u3092\u88dc\u9593\u3059\u308b\u3002.<\/p>\n

      \n

      SYBR Green\u3092\u8d85\u3048\u308b\u30d7\u30ed\u30fc\u30d6\u30d9\u30fc\u30b9\u306ePCR\u306e\u5229\u70b9\uff1a<\/strong><\/h2>\n
      \n
      \n\n\n\n\n\n\n\n\n
      \u7279\u5fb4<\/th>\nSYBR\u30b0\u30ea\u30fc\u30f3<\/th>\n\u30d7\u30ed\u30fc\u30d6\u30d9\u30fc\u30b9\uff08\u672c\u30ad\u30c3\u30c8\uff09<\/th>\n<\/tr>\n<\/thead>\n
      \u7279\u7570\u6027<\/td>\n\u3059\u3079\u3066\u306edsDNA\u3068\u7d50\u5408<\/td>\n\u7279\u5b9a\u306e\u6a19\u7684\u914d\u5217\u306b\u306e\u307f\u7d50\u5408\u3059\u308b<\/td>\n<\/tr>\n
      \u507d\u967d\u6027\u306e\u30ea\u30b9\u30af<\/td>\n\u3088\u308a\u9ad8\u3044<\/td>\n\u3088\u308a\u4f4e\u3044<\/td>\n<\/tr>\n
      \u30b3\u30b9\u30c8<\/td>\n\u3088\u308a\u4f4e\u3044<\/td>\n\u3088\u308a\u9ad8\u3044\uff08\u3057\u304b\u3057\u3001\u3088\u308a\u4fe1\u983c\u3067\u304d\u308b\uff09<\/td>\n<\/tr>\n
      \u4f7f\u3044\u3084\u3059\u3055<\/td>\n\u3088\u308a\u30b7\u30f3\u30d7\u30eb\u306b<\/td>\n\u30d7\u30ed\u30fc\u30d6\u8a2d\u8a08\u304c\u5fc5\u8981<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
      \n
      <\/div>\n<\/div>\n<\/div>\n<\/div>\n
      \n

      \u4f7f\u7528\u76ee\u7684<\/strong><\/h3>\n

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