{"id":410,"date":"2025-07-01T09:16:36","date_gmt":"2025-07-01T09:16:36","guid":{"rendered":"https:\/\/birdstesting.com\/?post_type=product&p=410"},"modified":"2025-07-01T09:17:17","modified_gmt":"2025-07-01T09:17:17","slug":"%e3%83%8f%e3%83%88%e3%83%bb%e3%83%91%e3%83%a9%e3%83%9f%e3%82%af%e3%82%bd%e3%82%a6%e3%82%a4%e3%83%ab%e3%82%b9%e6%a4%9c%e6%9f%bb%e3%82%ad%e3%83%83%e3%83%88","status":"publish","type":"product","link":"https:\/\/birdstesting.com\/ja\/%e3%83%8f%e3%83%88%e3%83%bb%e3%83%91%e3%83%a9%e3%83%9f%e3%82%af%e3%82%bd%e3%82%a6%e3%82%a4%e3%83%ab%e3%82%b9%e6%a4%9c%e6%9f%bb%e3%82%ad%e3%83%83%e3%83%88\/","title":{"rendered":"\u30cf\u30c8\u30d1\u30e9\u30df\u30af\u30bd\u30a6\u30a4\u30eb\u30b91\u578b\uff08PPMV-1\uff09RNA\u691c\u67fb\u30ad\u30c3\u30c8 - RT-qPCR"},"content":{"rendered":"

\u30ab\u30bf\u30ed\u30b0\u756a\u53f7<\/strong> AP2409006\u30b9\u30da\u30c3\u30af<\/strong> 50T \/ 100T\u30b9\u30c8\u30ec\u30fc\u30b8\uff1a<\/strong> -20\u00b0C<\/p>\n

\n

\u88fd\u54c1\u306e\u6982\u8981<\/strong><\/h3>\n

\u306b\u3064\u3044\u3066 \u30cf\u30c8\u30d1\u30e9\u30df\u30af\u30bd\u30a6\u30a4\u30eb\u30b91\u578b\uff08PPMV-1\uff09RNA\u691c\u51fa\u30ad\u30c3\u30c8<\/strong> \u3092\u540c\u5b9a\u3059\u308b\u305f\u3081\u306e\u9ad8\u611f\u5ea6RT-qPCR\u8a3a\u65ad\u30c4\u30fc\u30eb\u3067\u3042\u308b\u3002 \u30d4\u30fc\u30d4\u30fc\u30a8\u30e0\u30d6\u30a4\u30ef\u30f3<\/strong>(1)\u3001(2)\u3001(3)\u3001(4) RNA\u30a6\u30a4\u30eb\u30b9<\/strong> \u539f\u56e0 \u30cb\u30e5\u30fc\u30ab\u30c3\u30b9\u30eb\u75c5<\/em> \u30cf\u30c8\u306e\u5834\u5408\u3002\u611f\u67d3\u3057\u305f\u9ce5\u306e\u81e8\u5e8a\u75c7\u72b6\u306b\u306f\u4ee5\u4e0b\u306e\u3088\u3046\u306a\u3082\u306e\u304c\u3042\u308b\u3002 \u4e0b\u75e2\u3001\u4e0b\u80a2\u9ebb\u75fa\u3001\u632f\u6226\u3001\u795e\u7d4c\u75c7\u72b6<\/strong>.<\/p>\n

PPMV-1\u306f \u611f\u67d3\u529b\u304c\u5f37\u3044<\/strong>\u7f79\u60a3\u7387\u3068\u6b7b\u4ea1\u7387\u304c\u9ad8\u3044\u3002\u3053\u306e\u75c5\u6c17\u306f \u53e3\u8154\u3001\u547c\u5438\u5668\u3001\u773c\u3001\u751f\u6b96\u5668\u3001\u3055\u3089\u306b\u306f\u5275\u50b7\u306b\u95a2\u9023\u3059\u308b\u3002<\/strong> \u30eb\u30fc\u30c8\u30bf\u30a4\u30e0\u30ea\u30fc\u3067\u6b63\u78ba\u306a\u691c\u51fa\u306f\u3001\u75be\u75c5\u7ba1\u7406\u3001\u4e8c\u6b21\u611f\u67d3\u306e\u4e88\u9632\u3001\u30a2\u30a6\u30c8\u30d6\u30ec\u30a4\u30af\u306e\u5c01\u3058\u8fbc\u3081\u306b\u4e0d\u53ef\u6b20\u3067\u3042\u308b\u3002<\/p>\n

\u3053\u306e\u30ad\u30c3\u30c8\u306f\u3001PPMV-1\u306e\u4fdd\u5b58\u3055\u308c\u305f\u907a\u4f1d\u5b50\u914d\u5217\u306b\u57fa\u3065\u304d\u3001\u4ee5\u4e0b\u306e\u76ee\u7684\u306b\u6700\u9069\u5316\u3055\u308c\u3066\u3044\u308b\u3002 \u53e3\u8154\/\u9f3b\u8154\u30b9\u30ef\u30d6\u3001\u8840\u6db2\u3001\u7d44\u7e54\u3067\u306e\u8fc5\u901f\u691c\u51fa<\/strong> \u30cf\u30c8\u3084\u305d\u306e\u4ed6\u306e\u9ce5\u985e\u3092\u7d4c\u7531\u3057\u3066 \u4e00\u6bb5\u968e\u9006\u8ee2\u5199\u5b9a\u91cf PCR<\/a> (RT-qPCR\uff09<\/strong>.<\/p>\n

\n

\u4e3b\u306a\u7279\u5fb4<\/strong><\/h3>\n
    \n
  • \n

    \u30ef\u30f3\u30b9\u30c6\u30c3\u30d7RT-qPCR<\/strong> \u76f4\u63a5RNA\u691c\u51fa\u7528<\/p>\n<\/li>\n

  • \n

    \u5bfe\u8c61 \u30b9\u30ef\u30d6\u3001\u8840\u6db2\uff08EDTA\uff09\u3001\u7d44\u7e54\u30db\u30e2\u30b8\u30cd\u30fc\u30c8<\/strong><\/p>\n<\/li>\n

  • \n

    \u542b\u307e\u308c\u308b\u3082\u306e \u967d\u6027\u304a\u3088\u3073\u9670\u6027\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb<\/strong><\/p>\n<\/li>\n

  • \n

    \u901f\u3044\u7d50\u679c\u3060\u3001 \u9ad8\u611f\u5ea6\u3001\u9ad8\u7279\u7570\u6027<\/strong><\/p>\n<\/li>\n

  • \n

    \u9577\u671f\u4fdd\u5b58\u53ef\u80fd\u671f\u9593\uff1a-20\u2103\u306712\u30f6\u6708\u4ee5\u4e0a<\/p>\n<\/li>\n

  • \n

    \u9ce9\u30d6\u30ea\u30fc\u30c0\u30fc\u3001\u9ce5\u985e\u7814\u7a76\u6240\u3001\u7363\u533b\u76e3\u8996\u306b\u6700\u9069<\/p>\n<\/li>\n<\/ul>\n

    \n

    \u30ad\u30c3\u30c8\u306e\u69cb\u6210\u90e8\u54c1\uff1a<\/strong><\/h3>\n
    \n
    \n\n\n\n\n\n\n\n\n
    \u30b3\u30f3\u30dd\u30fc\u30cd\u30f3\u30c8<\/th>\n50T<\/th>\n100T<\/th>\n<\/tr>\n<\/thead>\n
    PPMV-1 \u53cd\u5fdc\u30d0\u30c3\u30d5\u30a1\u30fc<\/td>\n575 \u00b5L<\/td>\n1150 \u00b5L<\/td>\n<\/tr>\n
    PPMV-1 \u30d7\u30ec\u30df\u30c3\u30af\u30b9<\/td>\n575 \u00b5L<\/td>\n1150 \u00b5L<\/td>\n<\/tr>\n
    PPMV-1 \u967d\u6027\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb<\/td>\n100 \u00b5L<\/td>\n100 \u00b5L<\/td>\n<\/tr>\n
    \u9670\u6027\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb<\/td>\n100 \u00b5L<\/td>\n100 \u00b5L<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
    \n
    <\/div>\n<\/div>\n<\/div>\n<\/div>\n
    \n

    \u30b5\u30f3\u30d7\u30eb\u306e\u7a2e\u985e\u3068\u6761\u4ef6<\/strong><\/h3>\n
      \n
    • \n

      \u53e3\u8154\/\u54bd\u982d\u30b9\u30ef\u30d6<\/strong>:\u6ec5\u83cc\u7dbf\u68d2\uff1a2\uff5e8\u2103\uff0824\u6642\u9593\u4ee5\u5185\uff09\u307e\u305f\u306f-20\u2103\u3067\u4fdd\u5b58\u3002<\/p>\n<\/li>\n

    • \n

      \u5168\u8840<\/strong>:\u306e\u307f\u4f7f\u7528 EDTA\u6297\u51dd\u56fa\u5264<\/strong>\u30d8\u30d1\u30ea\u30f3\u3092\u907f\u3051\u30012\uff5e8\u2103\u30677\u65e5\u4ee5\u5185\u3001\u307e\u305f\u306f-20\u2103\u30673\u30f6\u6708\u4ee5\u5185\u306b\u4fdd\u5b58\u3059\u308b\u3002<\/p>\n<\/li>\n

    • \n

      \u7d44\u7e54<\/strong>\u65b0\u9bae\u306a\u7b4b\u8089\u307e\u305f\u306f\u81d3\u5668\u7d44\u7e54100 mg\u4ee5\u4e0b\u3092200-500 \u00b5L\u306e\u6ec5\u83cc\u6c34\u3067\u30db\u30e2\u30b8\u30ca\u30a4\u30ba\u3059\u308b\u3002<\/p>\n<\/li>\n<\/ul>\n

      RNA\u306f MD023 \u5b89\u5168\u306aRNA\u62bd\u51fa\u30ad\u30c3\u30c8<\/strong> \u307e\u305f\u306f\u540c\u7b49\u54c1\u3002\u62bd\u51fa\u3057\u305fRNA\u306f4\u2103\uff08\uff5e7\u65e5\uff09\u307e\u305f\u306f-20\u2103\uff08\uff5e6\u30f6\u6708\uff09\u3067\u4fdd\u5b58\u3059\u308b\u3002<\/p>\n

      \n

      RT-qPCR\u53cd\u5fdc\u30bb\u30c3\u30c8\u30a2\u30c3\u30d7\uff1a<\/strong><\/h3>\n
        \n
      1. \n

        \u8a66\u85ac\u3092\u89e3\u51cd\u3057\u3001\u77ed\u6642\u9593\u9060\u5fc3\u3059\u308b\u3002<\/p>\n<\/li>\n

      2. \n

        \u306b\u3064\u3044\u3066 n\u30b5\u30f3\u30d7\u30eb\uff0b2\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb<\/strong>\u30df\u30c3\u30af\u30b9\uff1a<\/p>\n

          \n
        • \n

          (n+2) \u00d7 11.5 \u00b5L PPMV-1 \u53cd\u5fdc\u30d0\u30c3\u30d5\u30a1\u30fc<\/strong><\/p>\n<\/li>\n

        • \n

          (n+2) \u00d7 11.5 \u00b5L PPMV-1 \u30d7\u30ec\u30df\u30c3\u30af\u30b9<\/strong><\/p>\n<\/li>\n<\/ul>\n<\/li>\n

        • \n

          \u3088\u304f\u6df7\u305c\u3066\u5206\u6ce8\u3059\u308b 23 \u00b5L<\/strong> PCR\u30c1\u30e5\u30fc\u30d6\u3042\u305f\u308a<\/p>\n<\/li>\n

        • \n

          \u8ffd\u52a0 \u30b5\u30f3\u30d7\u30ebRNA 2 \u00b5L<\/strong>, \u30dd\u30b8\u30c6\u30a3\u30d6<\/strong>\u3042\u308b\u3044\u306f \u30cd\u30ac\u30c6\u30a3\u30d6\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb<\/strong><\/p>\n<\/li>\n

        • \n

          \u6700\u7d42\u53cd\u5fdc\u91cf\uff1a 25 \u00b5L<\/strong><\/p>\n<\/li>\n<\/ol>\n

          \n

          PCR\u30b5\u30a4\u30af\u30ea\u30f3\u30b0\u6761\u4ef6\uff1a<\/strong><\/h3>\n
          \n
          \n\n\n\n\n\n\n\n\n
          \u30b9\u30c6\u30c3\u30d7<\/th>\n\u6e29\u5ea6<\/th>\n\u6642\u9593<\/th>\n<\/tr>\n<\/thead>\n
          \u9006\u8ee2\u5199<\/td>\n50\u00b0C<\/td>\n10\u5206<\/td>\n<\/tr>\n
          \u5909\u6027\u524d<\/td>\n95\u00b0C<\/td>\n2\u5206<\/td>\n<\/tr>\n
          PCR\u5897\u5e45\uff0840\u500d\uff09<\/td>\n95\u00b0C<\/td>\n5\u79d2<\/td>\n<\/tr>\n
          <\/td>\n59\u00b0C<\/td>\n35\u79d2\uff08FAM\u30c1\u30e3\u30f3\u30cd\u30eb\uff09<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
          \n
          <\/div>\n<\/div>\n<\/div>\n<\/div>\n
          \n

          ABI\u304a\u3088\u3073\u305d\u306e\u4ed6\u306e\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u30d7\u30e9\u30c3\u30c8\u30d5\u30a9\u30fc\u30e0\u306b\u5bfe\u5fdc\u3002ROX\u8272\u7d20\u306f\u30aa\u30d7\u30b7\u30e7\u30f3<\/p>\n<\/blockquote>\n

          \n

          \u7d50\u679c\u306e\u89e3\u91c8<\/strong><\/h3>\n
            \n
          • \n

            \u30dd\u30b8\u30c6\u30a3\u30d6<\/strong>:Ct\u226633\u3001\u660e\u78ba\u306a \"S \"\u30ab\u30fc\u30d6\u3092\u63cf\u304f<\/p>\n<\/li>\n

          • \n

            \u7591\u308f\u3057\u3044<\/strong>:33 < Ct < 38 \u2192\u518d\u691c\u67fb\u3092\u63a8\u5968<\/p>\n<\/li>\n

          • \n

            \u30cd\u30ac\u30c6\u30a3\u30d6<\/strong>:Ct\u226738 \u307e\u305f\u306f\u5897\u5e45\u66f2\u7dda\u306a\u3057<\/p>\n<\/li>\n<\/ul>\n

            \u7ba1\u7406\u8981\u4ef6<\/strong>:<\/p>\n

              \n
            • \n

              \u967d\u6027\u30b3\u30f3\u30c8\u30ed\u30fc\u30ebCt < 28<\/p>\n<\/li>\n

            • \n

              \u9670\u6027\u30b3\u30f3\u30c8\u30ed\u30fc\u30ebCt\u226738\u307e\u305f\u306fCt\u5024\u306a\u3057<\/p>\n<\/li>\n<\/ul>\n

              \n

              \u6ce8\u610f\u4e8b\u9805<\/strong><\/h3>\n
                \n
              1. \n

                \u5225\u3005\u306e\u4f5c\u696d\u30be\u30fc\u30f3\u3067\u884c\u3046\uff1aRNA\u524d\u51e6\u7406\u3001\u8a66\u85ac\u306e\u30bb\u30c3\u30c8\u30a2\u30c3\u30d7\u3001PCR\u5897\u5e45<\/p>\n<\/li>\n

              2. \n

                \u624b\u888b\u3001\u767d\u8863\u3092\u7740\u7528\u3057\u3001\u5404\u5206\u91ce\u3067\u5225\u3005\u306e\u30d4\u30da\u30c3\u30c8\u3084\u5668\u5177\u3092\u4f7f\u7528\u3059\u308b\u3002<\/p>\n<\/li>\n

              3. \n

                \u51cd\u7d50\u878d\u89e3\u306e\u7e70\u308a\u8fd4\u3057\u3092\u907f\u3051\u3001\u8a66\u85ac\u3092\u906e\u5149\u3059\u308b\u3002<\/p>\n<\/li>\n

              4. \n

                \u7814\u7a76\u76ee\u7684\u306e\u307f\u3002\u81e8\u5e8a\u8a3a\u65ad\u3092\u76ee\u7684\u3068\u3057\u305f\u3082\u306e\u3067\u306f\u306a\u3044<\/p>\n<\/li>\n

              5. \n

                \u30a2\u30eb\u30b3\u30fc\u30eb\u3001\u5869\u7d20\u3001\u7d2b\u5916\u7dda\u306a\u3069\u3067\u4f5c\u696d\u30b9\u30da\u30fc\u30b9\u3084\u5668\u5177\u3092\u5b9a\u671f\u7684\u306b\u6d88\u6bd2\u3059\u308b\u3002<\/p>\n<\/li>\n<\/ol>\n

                \n

                \ud83d\uded2 PPMV-1\u3092\u65e9\u671f\u304b\u3064\u52b9\u679c\u7684\u306b\u691c\u51fa\u3059\u308b\u3002<\/strong>
                \u7e41\u6b96\u696d\u8005\u3001\u8a3a\u65ad\u30e9\u30dc\u3001\u9ce5\u985e\u75be\u75c5\u7814\u7a76\u8005\u304c\u3001\u30cf\u30c8\u306e\u7fa4\u308c\u306e\u5065\u5eb7\u3092\u5b88\u308a\u3001\u30cf\u30c8\u30fb\u30d1\u30e9\u30df\u30af\u30bd\u30a6\u30a4\u30eb\u30b9\u306e\u767a\u751f\u306b\u3088\u308b\u6b7b\u4ea1\u30ea\u30b9\u30af\u3092\u4f4e\u6e1b\u3059\u308b\u305f\u3081\u306b\u4e0d\u53ef\u6b20\u306a\u30c4\u30fc\u30eb\u3002<\/p>","protected":false},"excerpt":{"rendered":"

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