{"id":410,"date":"2025-07-01T09:16:36","date_gmt":"2025-07-01T09:16:36","guid":{"rendered":"https:\/\/birdstesting.com\/?post_type=product&p=410"},"modified":"2025-07-01T09:17:17","modified_gmt":"2025-07-01T09:17:17","slug":"%eb%b9%84%eb%91%98%ea%b8%b0-%ed%8c%8c%eb%9d%bc%eb%af%b9%ec%86%8c%eb%b0%94%ec%9d%b4%eb%9f%ac%ec%8a%a4-%ed%85%8c%ec%8a%a4%ed%8a%b8-%ed%82%a4%ed%8a%b8","status":"publish","type":"product","link":"https:\/\/birdstesting.com\/ko\/%eb%b9%84%eb%91%98%ea%b8%b0-%ed%8c%8c%eb%9d%bc%eb%af%b9%ec%86%8c%eb%b0%94%ec%9d%b4%eb%9f%ac%ec%8a%a4-%ed%85%8c%ec%8a%a4%ed%8a%b8-%ed%82%a4%ed%8a%b8\/","title":{"rendered":"\ube44\ub458\uae30 \ud30c\ub77c\ubbf9\uc18c\ubc14\uc774\ub7ec\uc2a4 1\ud615(PPMV-1) RNA \ud14c\uc2a4\ud2b8 \ud0a4\ud2b8 - RT-qPCR"},"content":{"rendered":"

\uce74\ud0c8\ub85c\uadf8 \ubc88\ud638.:<\/strong> AP2409006\uc0ac\uc591:<\/strong> 50T \/ 100T\uc800\uc7a5\uc18c:<\/strong> -20\u00b0C<\/p>\n

\n

\uc81c\ud488 \uac1c\uc694:<\/strong><\/h3>\n

\uadf8\ub9ac\uace0 \ube44\ub458\uae30 \ud30c\ub77c\ubbf9\uc18c\ubc14\uc774\ub7ec\uc2a4 1\ud615(PPMV-1) RNA \uac80\ucd9c \ud0a4\ud2b8<\/strong> \ub294 \ub2e4\uc74c\uc744 \uc2dd\ubcc4\ud558\uae30 \uc704\ud55c \uace0\uac10\ub3c4 RT-qPCR \uc9c4\ub2e8 \ub3c4\uad6c\uc785\ub2c8\ub2e4. PPMV-1<\/strong>, \ubc0f RNA \ubc14\uc774\ub7ec\uc2a4<\/strong> \uadf8 \uc6d0\uc778\uc740 \ub274\uce90\uc2ac\ubcd1<\/em> \ube44\ub458\uae30\uc5d0\uc11c \ubc1c\uacac\ub418\uc5c8\uc2b5\ub2c8\ub2e4. \uac10\uc5fc\ub41c \uc870\ub958\uc758 \uc784\uc0c1 \uc99d\uc0c1\uc740 \ub2e4\uc74c\uacfc \uac19\uc2b5\ub2c8\ub2e4. \uc124\uc0ac, \ub2e4\ub9ac \ub9c8\ube44, \ub5a8\ub9bc \ubc0f \uc2e0\uacbd\ud559\uc801 \uc99d\uc0c1<\/strong>.<\/p>\n

PPMV-1\uc740 \uc804\uc5fc\uc131\uc774 \ub192\uc74c<\/strong>, \uc774\ud658\uc728\uacfc \uc0ac\ub9dd\ub960\uc774 \ub192\uc740 \uc9c8\ubcd1\uc785\ub2c8\ub2e4. \ub2e4\uc74c\uc744 \ud1b5\ud574 \ube60\ub974\uac8c \ud655\uc0b0\ub429\ub2c8\ub2e4. \uad6c\uac15, \ud638\ud761\uae30, \uc548\uad6c, \uc0dd\uc2dd\uae30, \uc2ec\uc9c0\uc5b4 \uc0c1\ucc98 \uad00\ub828\uae4c\uc9c0<\/strong> \uacbd\ub85c\ub97c \ucd94\uc801\ud569\ub2c8\ub2e4. \uc2dc\uae30\uc801\uc808\ud558\uace0 \uc815\ud655\ud55c \ud0d0\uc9c0\ub294 \uc9c8\ubcd1 \uad00\ub9ac, 2\ucc28 \uac10\uc5fc \uc608\ubc29, \uc9d1\ub2e8 \ubc1c\ubcd1 \uc5b5\uc81c\ub97c \uc704\ud574 \ud544\uc218\uc801\uc785\ub2c8\ub2e4.<\/p>\n

\uc774 \ud0a4\ud2b8\ub294 PPMV-1\uc758 \ubcf4\uc874\ub41c \uc720\uc804\uc790 \uc11c\uc5f4\uc744 \uae30\ubc18\uc73c\ub85c \ud558\uba70 \ub2e4\uc74c\uc5d0 \ucd5c\uc801\ud654\ub418\uc5c8\uc2b5\ub2c8\ub2e4. \uad6c\uac15\/\ubc30\ubcc0 \uba74\ubd09, \ud608\uc561 \ubc0f \uc870\uc9c1\uc5d0\uc11c \uc2e0\uc18d\ud55c \uac80\ucd9c<\/strong> \ube44\ub458\uae30 \ubc0f \uae30\ud0c0 \uc870\ub958 \uc885\uc744 \ud1b5\ud574 \uc6d0\uc2a4\ud15d \uc5ed\uc804\uc0ac \uc815\ub7c9\ud654 PCR<\/a> (RT-qPCR)<\/strong>.<\/p>\n

\n

\uc8fc\uc694 \uae30\ub2a5:<\/strong><\/h3>\n
    \n
  • \n

    \uc6d0\uc2a4\ud15d RT-qPCR<\/strong> \uc9c1\uc811 RNA \uac80\ucd9c\uc744 \uc704\ud55c<\/p>\n<\/li>\n

  • \n

    \uc801\uc6a9 \ub300\uc0c1 \uba74\ubd09, \ud608\uc561(EDTA), \uc870\uc9c1 \uade0\uc9c8\ud654\ubb3c<\/strong><\/p>\n<\/li>\n

  • \n

    \ud3ec\ud568 \uc0ac\ud56d \uc591\uc131 \ubc0f \uc74c\uc131 \ub300\uc870\uad70<\/strong><\/p>\n<\/li>\n

  • \n

    \ube60\ub978 \uacb0\uacfc, \ub192\uc740 \uac10\ub3c4 \ubc0f \ud2b9\uc774\uc131<\/strong><\/p>\n<\/li>\n

  • \n

    \uae34 \ubcf4\uad00 \uc218\uba85: -20\u00b0C\uc5d0\uc11c 12\uac1c\uc6d4 \uc774\uc0c1 \ubcf4\uad00 \uac00\ub2a5<\/p>\n<\/li>\n

  • \n

    \ube44\ub458\uae30 \uc0ac\uc721\uc790, \uc870\ub958 \uc2e4\ud5d8\uc2e4 \ubc0f \uc218\uc758\ud559 \uac10\uc2dc\uc5d0 \uc774\uc0c1\uc801\uc785\ub2c8\ub2e4.<\/p>\n<\/li>\n<\/ul>\n

    \n

    \ud0a4\ud2b8 \uad6c\uc131 \uc694\uc18c:<\/strong><\/h3>\n
    \n
    \n\n\n\n\n\n\n\n\n
    \uad6c\uc131 \uc694\uc18c<\/th>\n50T<\/th>\n100T<\/th>\n<\/tr>\n<\/thead>\n
    PPMV-1 \ubc18\uc751 \ubc84\ud37c<\/td>\n575 \u00b5L<\/td>\n1150 \u00b5L<\/td>\n<\/tr>\n
    PPMV-1 \ud504\ub9ac\ubbf9\uc2a4<\/td>\n575 \u00b5L<\/td>\n1150 \u00b5L<\/td>\n<\/tr>\n
    PPMV-1 \ud3ec\uc9c0\ud2f0\ube0c \uc81c\uc5b4<\/td>\n100 \u00b5L<\/td>\n100 \u00b5L<\/td>\n<\/tr>\n
    \ub124\uac70\ud2f0\ube0c \uc81c\uc5b4<\/td>\n100 \u00b5L<\/td>\n100 \u00b5L<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
    \n
    <\/div>\n<\/div>\n<\/div>\n<\/div>\n
    \n

    \uc0d8\ud50c \uc720\ud615 \ubc0f \uc694\uad6c \uc0ac\ud56d:<\/strong><\/h3>\n
      \n
    • \n

      \uad6c\uac15\/\ubc30\ubcc0 \uba74\ubd09<\/strong>: 2-8\u00b0C(24\uc2dc\uac04 \uc774\ub0b4) \ub610\ub294 -20\u00b0C\uc5d0\uc11c \ubcf4\uad00\ud55c \uba78\uade0 \uba74\ubd09<\/p>\n<\/li>\n

    • \n

      \uc804\ud608<\/strong>: \uc0ac\uc6a9 \uc804\uc6a9 EDTA \ud56d\uc751\uace0\uc81c<\/strong>, \ud5e4\ud30c\ub9b0\uc744 \ud53c\ud558\uace0 2-8\u00b0C\uc5d0\uc11c 7\uc77c \uc774\ub0b4 \ub610\ub294 -20\u00b0C\uc5d0\uc11c 3\uac1c\uc6d4 \uc774\ub0b4 \ubcf4\uad00\ud569\ub2c8\ub2e4.<\/p>\n<\/li>\n

    • \n

      \uc870\uc9c1<\/strong>200-500 \u00b5L \uba78\uade0\uc218\uc5d0 \uade0\uc9c8\ud654\ud55c \uc2e0\uc120\ud55c \uadfc\uc721 \ub610\ub294 \uc7a5\uae30 \uc870\uc9c1 \u2264100 mg<\/p>\n<\/li>\n<\/ul>\n

      RNA\ub97c \ucd94\ucd9c\ud558\ub824\uba74 MD023 \uc548\uc804\ud55c RNA \ucd94\ucd9c \ud0a4\ud2b8<\/strong> \ub610\ub294 \uc774\uc640 \ub3d9\ub4f1\ud55c \uc628\ub3c4. \ucd94\ucd9c\ud55c RNA\ub294 4\u00b0C(\u22647\uc77c) \ub610\ub294 -20\u00b0C(\u22646\uac1c\uc6d4)\uc5d0\uc11c \ubcf4\uad00\ud574\uc57c \ud569\ub2c8\ub2e4.<\/p>\n

      \n

      RT-qPCR \ubc18\uc751 \uc124\uc815:<\/strong><\/h3>\n
        \n
      1. \n

        \uc2dc\uc57d\uc744 \ud574\ub3d9\ud558\uace0 \uac04\ub2e8\ud788 \uc6d0\uc2ec\ubd84\ub9ac\ud569\ub2c8\ub2e4.<\/p>\n<\/li>\n

      2. \n

        For \uc0d8\ud50c \uac1c\uc218 + \ub300\uc870\uad70 2\uac1c<\/strong>, \ub97c \ud074\ub9ad\ud558\uace0 \ubbf9\uc2a4\ud569\ub2c8\ub2e4:<\/p>\n

          \n
        • \n

          (n+2) \u00d7 11.5\u00b5L PPMV-1 \ubc18\uc751 \ubc84\ud37c<\/strong><\/p>\n<\/li>\n

        • \n

          (n+2) \u00d7 11.5\u00b5L PPMV-1 \ud504\ub9ac\ubbf9\uc2a4<\/strong><\/p>\n<\/li>\n<\/ul>\n<\/li>\n

        • \n

          \uc798 \uc11e\uc5b4 \ubd84\ubc30\ud569\ub2c8\ub2e4. 23 \u00b5L<\/strong> PCR \ud29c\ube0c\ub2f9<\/p>\n<\/li>\n

        • \n

          \ucd94\uac00 2\u00b5L\uc758 \uc0d8\ud50c RNA<\/strong>, \uae0d\uc815\uc801<\/strong>\ub610\ub294 \ub124\uac70\ud2f0\ube0c \ucee8\ud2b8\ub864<\/strong><\/p>\n<\/li>\n

        • \n

          \ucd5c\uc885 \ubc18\uc751\ub7c9: 25 \u00b5L<\/strong><\/p>\n<\/li>\n<\/ol>\n

          \n

          PCR \uc0ac\uc774\ud074\ub9c1 \uc870\uac74:<\/strong><\/h3>\n
          \n
          \n\n\n\n\n\n\n\n\n
          \ub2e8\uacc4<\/th>\n\uc628\ub3c4<\/th>\n\uc2dc\uac04<\/th>\n<\/tr>\n<\/thead>\n
          \uc5ed\uc804\uc0ac<\/td>\n50\u00b0C<\/td>\n10\ubd84<\/td>\n<\/tr>\n
          \uc0ac\uc804 \ubcc0\uc131<\/td>\n95\u00b0C<\/td>\n2\ubd84<\/td>\n<\/tr>\n
          PCR \uc99d\ud3ed(\u00d740)<\/td>\n95\u00b0C<\/td>\n5\ucd08<\/td>\n<\/tr>\n
          <\/td>\n59\u00b0C<\/td>\n35\ucd08(FAM \ucc44\ub110)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
          \n
          <\/div>\n<\/div>\n<\/div>\n<\/div>\n
          \n

          ABI \ubc0f \uae30\ud0c0 \uc2e4\uc2dc\uac04 PCR \ud50c\ub7ab\ud3fc\uacfc \ud638\ud658\ub429\ub2c8\ub2e4. ROX \uc5fc\ub8cc \uc635\uc158.<\/p>\n<\/blockquote>\n

          \n

          \uacb0\uacfc \ud574\uc11d:<\/strong><\/h3>\n
            \n
          • \n

            \uae0d\uc815\uc801<\/strong>: Ct \u2264 33(\uba85\ud655\ud55c \u201cS\u201d \uace1\uc120)<\/p>\n<\/li>\n

          • \n

            \uc758\uc2ec\ub428<\/strong>: 33 < Ct < 38 \u2192 \uc7ac\uac80\uc0ac \uad8c\uc7a5<\/p>\n<\/li>\n

          • \n

            \ubd80\uc815\uc801<\/strong>: Ct \u2265 38 \ub610\ub294 \uc99d\ud3ed \uace1\uc120 \uc5c6\uc74c<\/p>\n<\/li>\n<\/ul>\n

            \uc81c\uc5b4 \uc694\uad6c \uc0ac\ud56d<\/strong>:<\/p>\n

              \n
            • \n

              \ud3ec\uc9c0\ud2f0\ube0c \ucee8\ud2b8\ub864: Ct < 28<\/p>\n<\/li>\n

            • \n

              \uc74c\uc131 \ub300\uc870\uad70: Ct \u2265 38 \ub610\ub294 Ct \uac12 \uc5c6\uc74c<\/p>\n<\/li>\n<\/ul>\n

              \n

              \uc8fc\uc758 \uc0ac\ud56d:<\/strong><\/h3>\n
                \n
              1. \n

                \ubcc4\ub3c4\uc758 \uc791\uc5c5 \uc601\uc5ed\uc5d0\uc11c \uc218\ud589\ud569\ub2c8\ub2e4: RNA \uc900\ube44, \uc2dc\uc57d \uc124\uc815 \ubc0f PCR \uc99d\ud3ed<\/p>\n<\/li>\n

              2. \n

                \uc7a5\uac11\uacfc \uc2e4\ud5d8\uc2e4 \uac00\uc6b4\uc744 \ucc29\uc6a9\ud558\uace0 \uac01 \uc601\uc5ed\ub9c8\ub2e4 \ubcc4\ub3c4\uc758 \ud53c\ud3ab\uacfc \uc7a5\ube44\ub97c \uc0ac\uc6a9\ud569\ub2c8\ub2e4.<\/p>\n<\/li>\n

              3. \n

                \ubc18\ubcf5\uc801\uc778 \ub3d9\uacb0-\ud574\ub3d9 \uc0ac\uc774\ud074\uc744 \ud53c\ud558\uace0 \uc2dc\uc57d\uc744 \ube5b\uc73c\ub85c\ubd80\ud130 \ubcf4\ud638\ud569\ub2c8\ub2e4.<\/p>\n<\/li>\n

              4. \n

                \uc5f0\uad6c\uc6a9\uc73c\ub85c\ub9cc \uc0ac\uc6a9\ud558\uba70 \uc784\uc0c1 \uc9c4\ub2e8\uc6a9\uc774 \uc544\ub2d9\ub2c8\ub2e4.<\/p>\n<\/li>\n

              5. \n

                \uc54c\ucf54\uc62c, \uc5fc\uc18c \ub610\ub294 \uc790\uc678\uc120\uc73c\ub85c \uc791\uc5c5 \uacf5\uac04\uacfc \uc7a5\ube44\ub97c \uc815\uae30\uc801\uc73c\ub85c \uc18c\ub3c5\ud569\ub2c8\ub2e4.<\/p>\n<\/li>\n<\/ol>\n

                \n

                \ud83d\uded2 PPMV-1\uc744 \uc870\uae30\uc5d0 \ud6a8\uacfc\uc801\uc73c\ub85c \ud0d0\uc9c0\ud558\uc138\uc694.<\/strong>
                \uc0ac\uc721\uc790, \uc9c4\ub2e8 \uc2e4\ud5d8\uc2e4, \uc870\ub958 \uc9c8\ubcd1 \uc5f0\uad6c\uc790\ub4e4\uc774 \ube44\ub458\uae30 \ud30c\ub77c\ubbf9\uc18c\ubc14\uc774\ub7ec\uc2a4 \ubc1c\uc0dd\uc73c\ub85c\ubd80\ud130 \ubb34\ub9ac\uc758 \uac74\uac15\uc744 \ubcf4\ud638\ud558\uace0 \ud3d0\uc0ac \uc704\ud5d8\uc744 \uc904\uc774\uae30 \uc704\ud55c \ud544\uc218 \ub3c4\uad6c\uc785\ub2c8\ub2e4.<\/p>","protected":false},"excerpt":{"rendered":"

                \uce74\ud0c8\ub85c\uadf8 \ubc88\ud638: AP2409006 \uc0ac\uc591: 50T \/ 100T \ubcf4\uad00: -20\u00b0C \uc81c\ud488 \uac1c\uc694: \ube44\ub458\uae30 \ud30c\ub77c\ubbf9\uc18c\ubc14\uc774\ub7ec\uc2a4 1\ud615(PPMV-1) RNA \uac80\ucd9c \ud0a4\ud2b8\ub294 \ube44\ub458\uae30 \ub274\uce90\uc2ac\ubcd1\uc744 \uc720\ubc1c\ud558\ub294 RNA \ubc14\uc774\ub7ec\uc2a4\uc778 PPMV-1\uc744 \uc2dd\ubcc4\ud558\uae30 \uc704\ud55c \uace0\uac10\ub3c4 RT-qPCR \uc9c4\ub2e8 \ub3c4\uad6c\uc785\ub2c8\ub2e4. \uac10\uc5fc\ub41c \uc870\ub958\uc758 \uc784\uc0c1 \uc99d\uc0c1\uc73c\ub85c\ub294 \uc124\uc0ac, \ub2e4\ub9ac \ub9c8\ube44, \ub5a8\ub9bc, \uc2e0\uacbd\ud559\uc801 \uc99d\uc0c1 \ub4f1\uc774 \uc788\uc2b5\ub2c8\ub2e4. PPMV-1\uc740 \uc804\uc5fc\uc131\uc774 \ub9e4\uc6b0 \ub192\uc73c\uba70...<\/p>","protected":false},"featured_media":411,"comment_status":"open","ping_status":"closed","template":"","meta":{"_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"default","_kad_post_title":"","_kad_post_layout":"default","_kad_post_sidebar_id":"","_kad_post_content_style":"default","_kad_post_vertical_padding":"default","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"_kad_post_classname":""},"product_brand":[126],"product_cat":[125],"product_tag":[],"class_list":["post-410","product","type-product","status-publish","has-post-thumbnail","product_brand-sanshi-biotech","product_cat-bird-dna-test","first","instock","shipping-taxable","product-type-simple","entry","content-bg","loop-entry"],"_links":{"self":[{"href":"https:\/\/birdstesting.com\/ko\/wp-json\/wp\/v2\/product\/410","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/birdstesting.com\/ko\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/birdstesting.com\/ko\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/birdstesting.com\/ko\/wp-json\/wp\/v2\/comments?post=410"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/birdstesting.com\/ko\/wp-json\/wp\/v2\/media\/411"}],"wp:attachment":[{"href":"https:\/\/birdstesting.com\/ko\/wp-json\/wp\/v2\/media?parent=410"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/birdstesting.com\/ko\/wp-json\/wp\/v2\/product_brand?post=410"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/birdstesting.com\/ko\/wp-json\/wp\/v2\/product_cat?post=410"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/birdstesting.com\/ko\/wp-json\/wp\/v2\/product_tag?post=410"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}