Kit de extração rápida de DNA genômico de aves

(For PCR Analysis)
Item No.: DP202
Specification: 200 Tests
Storage: 2–8°C

Product Overview

The Kit de extração rápida de DNA genômico de aves is specially formulated for the quick and efficient extraction of genomic DNA from a wide variety of avian and mammalian tissue samples. Compatible with fresh or frozen biological materials—including feathers, blood, saliva, cultured cells, mouse tails/ears—this kit delivers high-quality genomic DNA suitable for PCR-based genotyping and molecular detection.

Utilizing a non-column, enzyme-based lysis protocol, the kit eliminates the need for centrifugation columns or hazardous solvents, enabling rapid sample preparation in as little as 20 minutes. Ideal for use in bird species identification, genetic sexing, viral detection, and other PCR applications.

Key Features

• Fast: Complete DNA extraction in under 20 minutes.

• Versatile: Suitable for feathers, blood, saliva, and soft tissues.

• Safe: No phenol-chloroform, no column filtration needed.

• Convenient: Minimal sample input (as low as 10 µL or 10 mg).

• PCR-Ready: Compatible with conventional or real-time PCR workflows.

Kit Components

🧊 Storage: 2–8°C. Shelf life: 12 months.

Sample Compatibility

Protocol Summary

⚠️ Equipment Required: PCR machine or metal block heater (55°C & 95°C)

1. Prepare Digestion Buffer: Mix 50 µL Reagent A + 2.5 µL Reagent B per sample. Dispense 52 µL into PCR tubes.

2. Add Sample:

   • Penas: Cut finely at the shaft; place into the buffer.

   • Blood/Saliva: Add ≤10 µL directly to buffer.

   • Mouse Tissue: Mince ≤0.5 cm piece and mix well.

3. Incubate:

   • 55°C for 10 min (lysis step)

   • 95°C for 5 min (enzyme inactivation)

4. Cool & Centrifuge:

   • Chill at -20°C for 5 min or at 4°C for 15 min.

   • Briefly centrifuge and use the supernatant for PCR.

💡 Extracted DNA can be stored at 4°C for 1 month or -20°C for up to 1 year.

Precautions

• Mix all reagents thoroughly before use.

• Do not open PCR tubes immediately after high-temperature incubation.

• Wear gloves, masks, and lab coats to avoid contamination.

• Disinfect work surfaces and dispose of biological waste in disinfectant solution.

• For research use only. Not for clinical or diagnostic use.

Applications

• Bird DNA genotyping & teste de gênero

• Viral DNA detection (e.g., PBFDV, Herpesvirus)

• Genetic identification and STR-based parentage analysis

• Routine sample prep for PCR, qPCR, and sequencing workflows

Ordering Information