Kit de teste de DNA do circovírus do pombo (PiCV) para qPCR 50T / 100T

Product Description:

The Pigeon Circovirus (PiCV) DNA Test Kit is a specialized qPCR-based molecular diagnostic tool designed to detect PiCV, a major cause of young pigeon disease syndrome (YPDS) and immune suppression in racing and breeding pigeons. PiCV replicates rapidly in immune system cells, leading to weakened immunity, low lymphocyte counts, and increased susceptibility to secondary infections.

Using fluorescence-based real-time PCR, this kit enables fast, reliable, and sensitive detection of PiCV nucleic acids from pigeon oral or cloacal swabs, blood, or tissue samples. It’s ideal for breeders, fanciers, avian labs, and veterinary professionals.

Key Features:

• ✅ Accurate detection of Pigeon Circovirus (PiCV) in racing and fancy pigeons

• ✅ Covers oral/cloacal swabs, blood (EDTA), and liver tissue

• ✅ Easy-to-use real-time PCR protocol

• ✅ Includes internal positive and negative controls for result verification

• ✅ Validated for high specificity and sensitivity

• ✅ Available in 50T and 100T formats

• ✅ Shelf life: ≥12 months at –20°C

Kit Components:

PCR Reaction Setup:

• Reaction volume: 25 µL

• Mix: 23 µL Detection Reagent + 2 µL Sample DNA / Positive / Negative Control

• Real-time PCR cycling conditions:

  • 50°C for 2 min (UNG digestion)

  • 95°C for 5 min (denaturation)

  • 40 cycles of:

    • 95°C for 10 sec

    • 60°C for 35 sec (fluorescence read in FAM channel)

Compatible with ABI series and other qPCR instruments. ROX reference dye optional if needed.

Result Interpretation:

• Positive: Ct ≤ 33 with typical “S” curve

• Suspect: 33 < Ct < 38 (retest recommended to rule out inhibitors or contamination)

• Negative: Ct ≥ 38 or no amplification curve

Control results must validate test effectiveness. Follow the manual strictly.

Sample Requirements:

• Swabs: Oral or cloacal swabs placed into sterile centrifuge tubes

• Blood: EDTA anticoagulated whole blood (avoid heparin)

• Tissue: Fresh liver tissue (≤100mg), homogenized in sterile water

DNA extraction should be performed with a validated kit such as DP202. Extracted DNA should be stored at 4°C (≤7 days) or –20°C (≤6 months).

Important Notes:

• Use separate areas for sample prep, reaction setup, and amplification

• Prevent cross-contamination with clean gloves and tools

• Fully thaw reagents before use and avoid repeated freeze-thaw

• Protect from light and do not reuse components

• For research use only – not for clinical diagnostic purposes

🛒 Order the PiCV qPCR test kit today to ensure early detection and improve pigeon health management. Perfect for breeders, bird farms, and avian diagnostic labs.