{"id":307,"date":"2025-06-06T02:17:44","date_gmt":"2025-06-06T02:17:44","guid":{"rendered":"https:\/\/birdstesting.com\/?post_type=product&p=307"},"modified":"2025-06-19T07:31:04","modified_gmt":"2025-06-19T07:31:04","slug":"%e9%b3%a5%e9%a1%9e%e6%80%a7%e5%88%a5%e6%aa%a2%e6%b8%ac%e5%a5%97%e4%bb%b6-pcr-%e6%aa%a2%e6%b8%ac-48t-96t","status":"publish","type":"product","link":"https:\/\/birdstesting.com\/zh\/%e9%b3%a5%e9%a1%9e%e6%80%a7%e5%88%a5%e6%aa%a2%e6%b8%ac%e5%a5%97%e4%bb%b6-pcr-%e6%aa%a2%e6%b8%ac-48t-96t\/","title":{"rendered":"\u9ce5\u985e\u6027\u5225\u6aa2\u6e2c\u5957\u4ef6\uff08PCR \u6aa2\u6e2c\uff0948T\/96T"},"content":{"rendered":"

\u9805\u76ee\u7de8\u865f<\/strong>:AN2206001
\u898f\u683c<\/strong>:48 \u6e2c\u8a66 \/ 96 \u6e2c\u8a66
\u5132\u5b58<\/strong>: -20\u00b0C\uff0c12 \u500b\u6708\u4fdd\u8cea\u671f<\/p>\n

\n

\u6982\u8ff0<\/strong><\/h3>\n

\u7684 \u9ce5\u985e\u6027\u5225<\/a> \u6e2c\u8a66\u5957\u4ef6<\/a> (PCR \u6e2c\u8a66)<\/strong> \u662f\u4f7f\u7528\u5f9e\u7fbd\u6bdb\u3001\u8840\u6db2\u6216\u7d44\u7e54\u6a23\u672c\u4e2d\u8403\u53d6\u7684 DNA \u9451\u5b9a\u9ce5\u985e\u6027\u5225\u7684\u5c08\u696d\u53ef\u9760\u5de5\u5177\u3002\u672c\u5957\u4ef6\u5c08\u70ba\u975e\u9ce5\u985e\u54c1\u7a2e\u8a2d\u8a08\uff0c\u4f8b\u5982\u9e1a\u9d61 (Psittaciformes)\u3001\u9d3f\u5b50 (Columbiformes)\u3001\u96de (Galliformes)\u3001\u9e73 (Ciconiiformes)\u3001\u9db4 (Gruiformes) \u4ee5\u53ca\u67d0\u4e9b\u6c34\u79bd (Anseriformes)\uff0c\u53ef\u63d0\u4f9b\u4ee5\u4e0b\u529f\u80fd \u6e96\u78ba\u3001\u5feb\u901f\u4e14\u53ef\u91cd\u8907\u7684\u7d50\u679c<\/strong> \u901a\u904e PCR \u64f4\u589e\u3002<\/p>\n

\n

\u4e3b\u8981\u529f\u80fd<\/strong><\/h3>\n
    \n
  • \n

    \u2705 \u9ad8\u6e96\u78ba\u6027<\/strong>:\u900f\u904e\u6aa2\u6e2c\u6027\u5225\u7279\u7570\u6027\u57fa\u56e0\u5e36 (~700 bp \u53ca ~400 bp) \u4f86\u5340\u5206\u96cc\u96c4\u9ce5\u3002<\/p>\n<\/li>\n

  • \n

    \u2705 \u6a23\u54c1\u591a\u6a23\u6027<\/strong>:\u9069\u7528\u65bc\u7fbd\u6bdb\u3001\u8840\u6db2\u548c\u7d44\u7e54\u3002<\/p>\n<\/li>\n

  • \n

    \u2705 \u5373\u7528\u578b\u6df7\u5408\u6bcd\u6db2<\/strong>:\u5305\u62ec\u6297\u6291\u5236\u5291 2\u00d7 Taq PCR Master Mix\uff08\u542b\u67d3\u6599\uff09\u3002<\/p>\n<\/li>\n

  • \n

    \u2705 \u8f15\u9b06\u8b80\u53d6\u7d50\u679c<\/strong>:PCR \u7522\u54c1\u53ef\u76f4\u63a5\u900f\u904e\u74ca\u8102\u7cd6\u51dd\u81a0\u96fb\u6cf3\u9032\u884c\u5206\u6790\u3002<\/p>\n<\/li>\n

  • \n

    \u2705 \u7528\u65bc\u79d1\u5b78\u7814\u7a76<\/strong>:\u662f\u9ce5\u985e\u7e41\u6b96\u8005\u3001\u7814\u7a76\u4eba\u54e1\u548c\u91ce\u751f\u52d5\u7269\u4fdd\u8b77\u8005\u7684\u7406\u60f3\u9078\u64c7\u3002<\/p>\n<\/li>\n<\/ul>\n

    \n

    \u9ce5\u985e\u6027\u5225\u6e2c\u8a66 \u5957\u4ef6\u7d44\u4ef6\uff08100T\uff0c\u9805\u76ee AN2206001-02\uff09<\/strong><\/h3>\n
    \n
    \n\n\n\n\n\n\n\n
    \u7d44\u4ef6<\/th>\n\u9ad4\u7a4d<\/th>\n<\/tr>\n<\/thead>\n
    \u8a66\u5291 C<\/td>\n1 mL<\/td>\n<\/tr>\n
    \u8a66\u5291 D<\/td>\n1 mL<\/td>\n<\/tr>\n
    \u8a66\u5291 E<\/td>\n100 \u00b5L<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
    \n
    <\/div>\n<\/div>\n<\/div>\n<\/div>\n

    \u8a3b\uff1a\u5c0d\u65bc 48T \u5957\u4ef6\uff0c\u5143\u4ef6\u9ad4\u7a4d\u6703\u76f8\u61c9\u8abf\u6574\u3002<\/em><\/p>\n

    \n

    PCR \u6e2c\u8a66\u5de5\u4f5c\u6d41\u7a0b<\/strong><\/h3>\n

    1. \u6a23\u54c1\u88fd\u5099<\/strong><\/h4>\n

    \u4f7f\u7528 DNA \u62bd\u53d6\u5957\u4ef6\uff0c\u4f8b\u5982 \u4e09\u5e0c\u751f\u7269 DP202<\/strong> \u5f9e\u9ce5\u985e\u7fbd\u6bdb\u3001\u8840\u6db2\u6216\u7d44\u7e54\u4e2d\u63d0\u53d6\u57fa\u56e0\u7d44 DNA\u3002<\/p>\n

    2. \u53cd\u61c9\u8a2d\u5b9a<\/strong><\/h4>\n
      \n
    • \n

      \u6df7\u5408\uff1a\u6bcf\u500b\u53cd\u61c9 7.5 \u00b5L \u8a66\u5291 C + 10 \u00b5L \u8a66\u5291 D + 1 \u00b5L \u8a66\u5291 E\u3002<\/p>\n<\/li>\n

    • \n

      \u52a0\u5165 2 \u00b5L DNA \u6a23\u54c1\u3002<\/p>\n<\/li>\n

    • \n

      \u7e3d\u53cd\u61c9\u91cf\uff1a20 \u00b5L\u3002<\/p>\n<\/li>\n<\/ul>\n

      3. PCR \u8a08\u5283<\/strong><\/h4>\n
        \n
      • \n

        \u521d\u59cb\u8b8a\u6027\uff1a94\u00b0C\uff0c3 \u5206\u9418<\/p>\n<\/li>\n

      • \n

        32 \u5faa\u74b0\uff1a<\/p>\n

          \n
        • \n

          \u8b8a\u6027\uff1a94\u00b0C\uff0c30 \u79d2<\/p>\n<\/li>\n

        • \n

          \u9000\u706b\uff1a50\u00b0C\uff0c45 \u79d2<\/p>\n<\/li>\n

        • \n

          \u5ef6\u5c55\uff1a72\u00b0C, 50 \u79d2<\/p>\n<\/li>\n<\/ul>\n<\/li>\n

        • \n

          \u6700\u5f8c\u5ef6\u9577\uff1a72\u00b0C\uff0c8 \u5206\u9418<\/p>\n<\/li>\n

        • \n

          \u4fdd\u6301\uff1a4\u00b0C<\/p>\n<\/li>\n<\/ul>\n

          4. \u51dd\u81a0\u96fb\u6cf3<\/strong><\/h4>\n

          \u4f7f\u7528 TAE \u6216 TBE \u7de9\u885d\u6db2\u4e2d\u7684 1%-1.5% \u743c\u8102\u7cd6\u51dd\u81a0\u4f86\u986f\u73fe PCR \u689d\u5e36\uff1a<\/p>\n

            \n
          • \n

            \u55ae\u4e00 ~700 bp \u5e36<\/strong>:\u7537\u6027<\/p>\n<\/li>\n

          • \n

            ~700 bp \u548c ~400 bp \u6216\u53ea\u6709 400 bp<\/strong>:\u5973\u6027<\/p>\n<\/li>\n

          • \n

            \u7121\u983b\u5e36<\/strong>:\u91cd\u65b0\u6e2c\u8a66\u6216\u8aee\u8a62\u6280\u8853\u652f\u63f4<\/p>\n<\/li>\n<\/ul>\n

            \n

            \u91cd\u8981\u6ce8\u610f\u4e8b\u9805<\/strong><\/h3>\n
              \n
            • \n

              \u4fdd\u6301\u7368\u7acb\u7684\u6a23\u54c1\u6e96\u5099\u3001\u53cd\u61c9\u8a2d\u7f6e\u548c\u7522\u54c1\u5206\u6790\u5340\u57df\uff0c\u4ee5\u907f\u514d\u6c61\u67d3\u3002<\/p>\n<\/li>\n

            • \n

              \u6234\u624b\u5957\u548c\u7a7f\u767d\u5927\u8902\uff1b\u5b9a\u671f\u6d88\u6bd2\u5de5\u5177\u3002<\/p>\n<\/li>\n

            • \n

              \u64f4\u589e\u5f8c\u4e0d\u8981\u7acb\u5373\u6253\u958b PCR \u7ba1\uff0c\u4ee5\u9632\u6b62\u9727\u5316\u6c61\u67d3\u3002<\/p>\n<\/li>\n<\/ul>\n

              \n

              \u61c9\u7528<\/strong><\/h3>\n

              \u6b64 PCR \u9ce5\u985e\u6027\u5225\u6aa2\u6e2c\u8a66\u5291\u76d2\u9069\u7528\u65bc\uff1a<\/p>\n

                \n
              • \n

                \u79bd\u9ce5\u7814\u7a76\u5be6\u9a57\u5ba4<\/strong><\/p>\n<\/li>\n

              • \n

                \u5bf5\u7269\u9ce5\u7e41\u6b96\u8005<\/strong><\/p>\n<\/li>\n

              • \n

                \u52d5\u7269\u5712\u548c\u91ce\u751f\u52d5\u7269\u76e3\u6e2c<\/strong><\/p>\n<\/li>\n

              • \n

                \u822a\u7a7a\u990a\u6b96\u4f01\u696d<\/strong><\/p>\n<\/li>\n<\/ul>\n

                \n

                \u2757 \u50c5\u4f9b\u7814\u7a76\u4f7f\u7528<\/strong>.\u4e0d\u64ec\u7528\u65bc\u81e8\u5e8a\u8a3a\u65b7\u3002<\/p>\n<\/blockquote>","protected":false},"excerpt":{"rendered":"

                \u9805\u76ee\u7de8\u865f: AN2206001\u898f\u683c\uff1a48 \u6b21\u6e2c\u8a66 \/ 96 \u6b21\u6e2c\u8a66\u5132\u5b58\u6eab\u5ea6\uff1a-20\u00b0C\uff0c12 \u500b\u6708\u4fdd\u8cea\u671f \u6982\u89bd \u9ce5\u985e\u6027\u5225\u6e2c\u8a66\u5957\u4ef6\uff08PCR \u6e2c\u8a66\uff09\u662f\u4e00\u7a2e\u5c08\u696d\u53ef\u9760\u7684\u5de5\u5177\uff0c\u53ef\u4f7f\u7528\u5f9e\u7fbd\u6bdb\u3001\u8840\u6db2\u6216\u7d44\u7e54\u6a23\u672c\u4e2d\u63d0\u53d6\u7684 DNA \u4f86\u9452\u5b9a\u9ce5\u985e\u7684\u6027\u5225\u3002<\/p>","protected":false},"featured_media":360,"comment_status":"open","ping_status":"closed","template":"","meta":{"_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"default","_kad_post_title":"","_kad_post_layout":"default","_kad_post_sidebar_id":"","_kad_post_content_style":"default","_kad_post_vertical_padding":"default","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"_kad_post_classname":""},"product_brand":[126],"product_cat":[15,127],"product_tag":[],"class_list":["post-307","product","type-product","status-publish","has-post-thumbnail","product_brand-sanshi-biotech","product_cat-bird-gender-test","product_cat-bird-dna-test-service","first","instock","shipping-taxable","product-type-simple","entry","content-bg","loop-entry"],"_links":{"self":[{"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/product\/307","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/comments?post=307"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/media\/360"}],"wp:attachment":[{"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/media?parent=307"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/product_brand?post=307"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/product_cat?post=307"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/product_tag?post=307"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}