{"id":387,"date":"2025-06-25T07:57:56","date_gmt":"2025-06-25T07:57:56","guid":{"rendered":"https:\/\/birdstesting.com\/?post_type=product&p=387"},"modified":"2025-06-25T07:59:33","modified_gmt":"2025-06-25T07:59:33","slug":"%e9%b4%bf%e7%92%b0%e7%8b%80%e7%97%85%e6%af%92-picv-dna-%e6%b8%ac%e8%a9%a6%e5%a5%97%e4%bb%b6","status":"publish","type":"product","link":"https:\/\/birdstesting.com\/zh\/%e9%b4%bf%e7%92%b0%e7%8b%80%e7%97%85%e6%af%92-picv-dna-%e6%b8%ac%e8%a9%a6%e5%a5%97%e4%bb%b6\/","title":{"rendered":"\u9069\u7528\u65bc qPCR 50T \/ 100T \u7684\u9d3f\u5713\u74b0\u75c5\u6bd2 (PiCV) DNA \u6e2c\u8a66\u5957\u4ef6"},"content":{"rendered":"

\u7522\u54c1\u8aaa\u660e\uff1a<\/h3>\n

\u7684 \u9d3f\u5b50\u5713\u75c5\u6bd2 (PiCV) DNA \u6e2c\u8a66<\/a> \u5957\u4ef6<\/strong> \u662f\u57fa\u65bc qPCR \u7684\u5c08\u9580\u5206\u5b50\u8a3a\u65b7\u5de5\u5177\uff0c\u65e8\u5728\u6aa2\u6e2c PiCV<\/strong>\uff0c\u662f\u5c0e\u81f4 \u5e7c\u9d3f\u75be\u75c5\u7d9c\u5408\u75c7 (YPDS)<\/strong> \u4ee5\u53ca\u8cfd\u9d3f\u548c\u7a2e\u9d3f\u7684\u514d\u75ab\u6291\u5236\u3002PiCV \u6703\u5728\u514d\u75ab\u7cfb\u7d71\u7d30\u80de\u4e2d\u5feb\u901f\u8907\u88fd\uff0c\u5c0e\u81f4\u514d\u75ab\u529b\u6e1b\u5f31\u3001\u6dcb\u5df4\u7d30\u80de\u6578\u91cf\u8b8a\u4f4e\uff0c\u4e26\u589e\u52a0\u5c0d\u4e8c\u6b21\u611f\u67d3\u7684\u6613\u611f\u6027\u3002<\/p>\n

\u672c\u8a66\u5291\u76d2\u4f7f\u7528\u4ee5\u87a2\u5149\u70ba\u57fa\u790e\u7684\u5373\u6642 PCR\uff0c\u53ef\u5f9e\u9d3f\u5b50\u53e3\u8154\u6216\u6cc4\u6b96\u8154\u62ed\u5b50\u3001\u8840\u6db2\u6216\u7d44\u7e54\u6a23\u672c\u4e2d\u5feb\u901f\u3001\u53ef\u9760\u3001\u9748\u654f\u5730\u6aa2\u6e2c PiCV \u6838\u9178\u3002\u5b83\u662f\u98fc\u990a\u8005\u3001\u9d3f\u53cb\u3001\u9ce5\u985e\u5be6\u9a57\u5ba4\u548c\u7378\u91ab\u5c08\u696d\u4eba\u58eb\u7684\u7406\u60f3\u9078\u64c7\u3002<\/p>\n

\n

\u4e3b\u8981\u529f\u80fd\uff1a<\/h3>\n
    \n
  • \n

    \u2705 \u7cbe\u78ba\u5075\u6e2c \u9d3f\u5b50\u5713\u75c5\u6bd2 (PiCV)<\/strong> \u8cfd\u9d3f\u548c\u82b1\u5f0f\u9d3f\u5b50<\/p>\n<\/li>\n

  • \n

    \u2705 \u6db5\u84cb\u53e3\u8154\/\u53e3\u8154\u62ed\u5b50\u3001\u8840\u6db2\uff08EDTA\uff09\u548c\u809d\u81df\u7d44\u7e54<\/p>\n<\/li>\n

  • \n

    \u2705 \u6613\u65bc\u4f7f\u7528\u7684\u5373\u6642 PCR \u5354\u5b9a<\/p>\n<\/li>\n

  • \n

    \u2705 \u5305\u62ec\u5167\u90e8\u967d\u6027\u548c\u9670\u6027\u5c0d\u7167\uff0c\u7528\u65bc\u9a57\u8b49\u7d50\u679c<\/p>\n<\/li>\n

  • \n

    \u2705 \u7d93\u9a57\u8b49\u5177\u6709\u9ad8\u5ea6\u7684\u7279\u7570\u6027\u548c\u9748\u654f\u5ea6<\/p>\n<\/li>\n

  • \n

    \u2705 \u63d0\u4f9b 50T \u548c 100T \u5169\u7a2e\u898f\u683c<\/p>\n<\/li>\n

  • \n

    \u2705 \u4fdd\u8cea\u671f\uff1a\u226512 \u500b\u6708\u65bc -20\u00b0C \u4e0b<\/p>\n<\/li>\n<\/ul>\n

    \n

    \u5957\u4ef6\u7d44\u4ef6\uff1a<\/h3>\n
    \n
    \n\n\n\n\n\n\n\n
    \u7d44\u4ef6<\/th>\n50T<\/th>\n100T<\/th>\n<\/tr>\n<\/thead>\n
    PiCV \u6aa2\u6e2c\u8a66\u5291<\/td>\n1150 \u00b5L<\/td>\n2 \u00d7 1150 \u00b5L<\/td>\n<\/tr>\n
    PiCV \u967d\u6027\u5c0d\u7167<\/td>\n100 \u00b5L<\/td>\n100 \u00b5L<\/td>\n<\/tr>\n
    \u9670\u6027\u5c0d\u7167<\/td>\n100 \u00b5L<\/td>\n100 \u00b5L<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
    \n
    <\/div>\n<\/div>\n<\/div>\n<\/div>\n
    \n

    PCR \u53cd\u61c9\u8a2d\u5b9a\uff1a<\/h3>\n
      \n
    • \n

      \u53cd\u61c9\u5bb9\u91cf\uff1a25 \u00b5L<\/p>\n<\/li>\n

    • \n

      \u6df7\u5408\uff1a23 \u00b5L \u6aa2\u6e2c\u8a66\u5291 + 2 \u00b5L \u6a23\u54c1 DNA \/ \u967d\u6027 \/ \u9670\u6027\u5c0d\u7167<\/p>\n<\/li>\n

    • \n

      Real-time PCR \u5faa\u74b0\u689d\u4ef6\uff1a<\/p>\n

        \n
      • \n

        50\u00b0C 2 \u5206\u9418 (UNG \u6d88\u89e3)<\/p>\n<\/li>\n

      • \n

        95\u00b0C 5 \u5206\u9418\uff08\u8b8a\u6027\uff09<\/p>\n<\/li>\n

      • \n

        40 \u5faa\u74b0\u7684\uff1a<\/p>\n

          \n
        • \n

          95\u00b0C 10 \u79d2<\/p>\n<\/li>\n

        • \n

          60\u00b0C 35 \u79d2\uff08FAM \u901a\u9053\u4e2d\u7684\u87a2\u5149\u8b80\u6578\uff09<\/p>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n

          \n

          \u8207 ABI \u7cfb\u5217\u53ca\u5176\u4ed6 qPCR \u5100\u5668\u76f8\u5bb9\u3002\u5982\u6709\u9700\u8981\uff0c\u53ef\u9078\u8cfc ROX \u53c3\u8003\u67d3\u6599\u3002<\/p>\n<\/blockquote>\n

          \n

          \u7d50\u679c\u95e1\u91cb\uff1a<\/h3>\n
            \n
          • \n

            \u6b63\u9762\uff1a<\/strong> Ct \u2264 33\uff0c\u5177\u6709\u5178\u578b\u7684 \"S \"\u66f2\u7dda<\/p>\n<\/li>\n

          • \n

            \u5acc\u7591\u72af<\/strong> 33 < Ct < 38 (\u5efa\u8b70\u91cd\u65b0\u6aa2\u6e2c\u4ee5\u6392\u9664\u6291\u5236\u5291\u6216\u6c61\u67d3)<\/p>\n<\/li>\n

          • \n

            \u8ca0\u9762\uff1a<\/strong> Ct \u2265 38 \u6216\u7121\u64f4\u589e\u66f2\u7dda<\/p>\n<\/li>\n<\/ul>\n

            \n

            \u63a7\u5236\u7d50\u679c\u5fc5\u9808\u9a57\u8b49\u6e2c\u8a66\u7684\u6709\u6548\u6027\u3002\u56b4\u683c\u9075\u5faa\u624b\u518a\u3002<\/p>\n<\/blockquote>\n

            \n

            \u6a23\u54c1\u9700\u6c42\uff1a<\/h3>\n
              \n
            • \n

              \u68c9\u82b1\u68d2\uff1a<\/strong> \u53e3\u8154\u6216\u6cc4\u6b96\u8154\u62ed\u5b50\u653e\u5165\u7121\u83cc\u96e2\u5fc3\u7ba1\u4e2d<\/p>\n<\/li>\n

            • \n

              \u8840\u6db2\uff1a<\/strong> EDTA \u6297\u51dd\u5168\u8840\uff08\u907f\u514d\u4f7f\u7528\u809d\u7d20\uff09<\/p>\n<\/li>\n

            • \n

              \u7d44\u7e54\uff1a<\/strong> \u65b0\u9bae\u809d\u81df\u7d44\u7e54\uff08\u2264100 \u6beb\u514b\uff09\uff0c\u5728\u7121\u83cc\u6c34\u4e2d\u5747\u8cea<\/p>\n<\/li>\n<\/ul>\n

              DNA \u8403\u53d6\u61c9\u4f7f\u7528\u7d93\u904e\u9a57\u8b49\u7684\u8a66\u5291\u76d2\uff0c\u4f8b\u5982 DP202<\/strong>.\u63d0\u53d6\u7684 DNA \u61c9\u5132\u5b58\u65bc 4\u00b0C (\u22647\u5929) \u6216 -20\u00b0C (\u22646\u500b\u6708)\u3002<\/p>\n

              \n

              \u91cd\u8981\u6ce8\u610f\u4e8b\u9805\uff1a<\/h3>\n
                \n
              • \n

                \u4f7f\u7528\u7368\u7acb\u7684\u5340\u57df\u9032\u884c\u6a23\u54c1\u6e96\u5099\u3001\u53cd\u61c9\u8a2d\u5b9a\u548c\u64f4\u5927<\/p>\n<\/li>\n

              • \n

                \u4f7f\u7528\u4e7e\u6de8\u7684\u624b\u5957\u548c\u5de5\u5177\u9632\u6b62\u4ea4\u53c9\u611f\u67d3<\/p>\n<\/li>\n

              • \n

                \u4f7f\u7528\u524d\u5c07\u8a66\u5291\u5b8c\u5168\u89e3\u51cd\uff0c\u907f\u514d\u91cd\u8907\u51cd\u878d<\/p>\n<\/li>\n

              • \n

                \u907f\u514d\u5149\u7dda\u7167\u5c04\uff0c\u8acb\u52ff\u91cd\u8907\u4f7f\u7528\u5143\u4ef6<\/p>\n<\/li>\n

              • \n

                \u50c5\u4f9b\u7814\u7a76\u4f7f\u7528 - \u4e0d\u4f5c\u81e8\u5e8a\u8a3a\u65b7\u7528\u9014<\/strong><\/p>\n<\/li>\n<\/ul>\n

                \n

                \ud83d\uded2 \u7acb\u5373\u8a02\u8cfc PiCV qPCR \u6e2c\u8a66\u5957\u4ef6<\/strong> \u4ee5\u78ba\u4fdd\u65e9\u671f\u6aa2\u6e2c\u4e26\u6539\u5584\u9d3f\u5b50\u5065\u5eb7\u7ba1\u7406\u3002\u975e\u5e38\u9069\u5408\u98fc\u990a\u8005\u3001\u9d3f\u5834\u548c\u79bd\u9ce5\u8a3a\u65b7\u5be6\u9a57\u5ba4\u4f7f\u7528\u3002<\/p>","protected":false},"excerpt":{"rendered":"

                \u7522\u54c1\u8aaa\u660e\uff1aPigeon Circovirus (PiCV) DNA Test Kit \u662f\u5c08\u9580\u7528\u4f86\u6aa2\u6e2c PiCV \u7684 qPCR \u5206\u5b50\u8a3a\u65b7\u5de5\u5177\uff0cPiCV \u662f\u9020\u6210\u8cfd\u9d3f\u548c\u7a2e\u9d3f\u5e7c\u9d3f\u75be\u75c5\u75c7\u5019\u7fa4 (YPDS) \u548c\u514d\u75ab\u6291\u5236\u7684\u4e3b\u8981\u539f\u56e0\u3002PiCV \u6703\u5728\u514d\u75ab\u7cfb\u7d71\u7d30\u80de\u4e2d\u5feb\u901f\u8907\u88fd\uff0c\u5c0e\u81f4\u514d\u75ab\u529b\u6e1b\u5f31\u3001\u6dcb\u5df4\u7d30\u80de\u6578\u91cf\u964d\u4f4e\uff0c\u4e26\u589e\u52a0\u5c0d...<\/p>","protected":false},"featured_media":388,"comment_status":"open","ping_status":"closed","template":"","meta":{"_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"default","_kad_post_title":"","_kad_post_layout":"default","_kad_post_sidebar_id":"","_kad_post_content_style":"default","_kad_post_vertical_padding":"default","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"_kad_post_classname":""},"product_brand":[126],"product_cat":[125],"product_tag":[],"class_list":["post-387","product","type-product","status-publish","has-post-thumbnail","product_brand-sanshi-biotech","product_cat-bird-dna-test","first","instock","shipping-taxable","product-type-simple","entry","content-bg","loop-entry"],"_links":{"self":[{"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/product\/387","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/comments?post=387"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/media\/388"}],"wp:attachment":[{"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/media?parent=387"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/product_brand?post=387"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/product_cat?post=387"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/product_tag?post=387"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}