{"id":397,"date":"2025-06-29T03:20:45","date_gmt":"2025-06-29T03:20:45","guid":{"rendered":"https:\/\/birdstesting.com\/?post_type=product&p=397"},"modified":"2025-06-29T03:20:46","modified_gmt":"2025-06-29T03:20:46","slug":"%e6%b2%99%e9%96%80%e6%b0%8f%e8%8f%8c-ung-%e6%a0%b8%e9%85%b8%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92","status":"publish","type":"product","link":"https:\/\/birdstesting.com\/zh\/%e6%b2%99%e9%96%80%e6%b0%8f%e8%8f%8c-ung-%e6%a0%b8%e9%85%b8%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92\/","title":{"rendered":"\u6c99\u9580\u6c0f\u83cc UNG \u6838\u9178\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08Real-Time PCR 50T\/100T\uff09"},"content":{"rendered":"

\u76ee\u9304\u7de8\u865f\uff1a<\/strong> AP2409004
\u898f\u683c\uff1a<\/strong> 50 \u6e2c\u8a66 \/ 100 \u6e2c\u8a66
\u5132\u5b58\uff1a<\/strong> -20\u00b0C<\/p>\n

\n

\u7522\u54c1\u8aaa\u660e<\/strong><\/h3>\n

\u7684 \u6c99\u9580\u6c0f\u83cc\u6838\u9178\u6aa2\u6e2c\u8a66\u5291\u76d2<\/strong> \u662f\u5373\u6642 PCR<\/a> \u5c08\u70ba\u5feb\u901f\u3001\u7279\u7570\u4e14\u9748\u654f\u5730\u6aa2\u6e2c \u6c99\u9580\u6c0f\u83cc DNA<\/strong> \u5728\u591a\u7a2e\u6a23\u672c\u985e\u578b\u4e2d\uff0c\u5305\u62ec\u7cde\u4fbf\u3001\u6c34\u3001\u7d44\u7e54\u548c\u98df\u7269\u3002\u6c99\u9580\u6c0f\u83cc\u662f\u4e00\u7a2e\u4e3b\u8981\u7684\u98df\u6e90\u6027\u75c5\u539f\u83cc\uff0c\u901a\u5e38\u7d93\u7531\u53d7\u6c61\u67d3\u7684\u86cb\u3001\u5bb6\u79bd\u548c\u8089\u985e\u50b3\u64ad\u3002<\/p>\n

\u672c\u5957\u4ef6\u4f7f\u7528 \u4ee5\u87a2\u5149\u63a2\u91dd\u70ba\u57fa\u790e\u7684\u5b9a\u91cf PCR (qPCR)<\/strong> \u6280\u8853\u4f86\u64f4\u589e\u6c99\u9580\u6c0f\u83cc\u57fa\u56e0\u7d44\u7684\u7279\u5b9a\u7247\u6bb5\u3002\u7d50\u679c\u7531 Ct\uff08\u9031\u671f\u81e8\u754c\u503c\uff09\u503c\u6c7a\u5b9a\uff0c\u6216\u900f\u904e\u6a19\u6e96\u66f2\u7dda\u5b9a\u91cf\u4f86\u4f30\u8a08\u6a23\u672c\u4e2d\u7684\u7d30\u83cc\u6578\u91cf\u3002<\/p>\n

\n

\u4e3b\u8981\u529f\u80fd<\/strong><\/h3>\n
    \n
  • \n

    \u2705 \u9ad8\u7279\u7570\u6027\u548c\u9ad8\u9748\u654f\u5ea6<\/strong> \u4f7f\u7528 TaqMan \u87a2\u5149\u63a2\u91dd\u65b9\u6cd5<\/p>\n<\/li>\n

  • \n

    \u8207\u591a\u7a2e\u5373\u6642 PCR \u7cfb\u7d71\u76f8\u5bb9 (FAM \u901a\u9053)<\/p>\n<\/li>\n

  • \n

    \u2705 \u5305\u62ec\u967d\u6027\u548c\u9670\u6027\u5c0d\u7167\uff0c\u7528\u65bc\u7d50\u679c\u9a57\u8b49<\/p>\n<\/li>\n

  • \n

    \u2705 \u542b\u6709 UNG \u9175\u7d20\uff0c\u53ef\u6d88\u9664\u651c\u5e36\u6c61\u67d3<\/p>\n<\/li>\n

  • \n

    \u2705 \u9069\u7528\u65bc\u98df\u54c1\u5b89\u5168\u76e3\u63a7\u3001\u52d5\u7269\u75be\u75c5\u8a3a\u65b7\u548c\u7814\u7a76<\/p>\n<\/li>\n<\/ul>\n

    \n

    \u5957\u4ef6\u7d44\u4ef6<\/strong><\/h3>\n
    \n
    \n\n\n\n\n\n\n\n
    \u7d44\u4ef6<\/th>\nAP2409004-02 (50T)<\/th>\nAP2409004-03 (100T)<\/th>\n<\/tr>\n<\/thead>\n
    \u6c99\u9580\u6c0f\u83cc PCR \u6df7\u5408\u6db2<\/td>\n1150 \u03bcL<\/td>\n2 \u00d7 1150 \u03bcL<\/td>\n<\/tr>\n
    \u967d\u6027\u5c0d\u7167<\/td>\n100 \u03bcL<\/td>\n100 \u03bcL<\/td>\n<\/tr>\n
    \u9670\u6027\u5c0d\u7167<\/td>\n100 \u03bcL<\/td>\n100 \u03bcL<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
    \n
    <\/div>\n<\/div>\n<\/div>\n<\/div>\n
    \n

    \u6a23\u54c1\u985e\u578b\u8207\u88fd\u5099<\/strong><\/h3>\n
      \n
    • \n

      \u62ed\u5b50\uff08\u53e3\u8154\/\u9670\u9053\uff09<\/strong>:\u5c0d\u65bc\u9ce5\u985e\u548c\u5bb6\u79bd\uff0c\u5c07\u7121\u83cc\u68c9\u82b1\u68d2\u63d2\u5165\u5589\u56a8\u548c\u6cc4\u6b96\u8154\uff0c\u8f15\u8f15\u8f49\u52d5\uff0c\u7136\u5f8c\u8f49\u5165\u7121\u83cc\u96e2\u5fc3\u7ba1\u4e2d\u3002<\/p>\n<\/li>\n

    • \n

      \u5168\u8840<\/strong>:\u4f7f\u7528 \u50c5 EDTA \u6297\u51dd\u5291<\/strong>.\u8acb\u52ff\u4f7f\u7528\u809d\u7d20\u3002\u5efa\u8b70\u4f7f\u7528\u65b0\u9bae\u8840\u6db2\u3002\u5132\u5b58\u65bc 2-8\u00b0C \u53ef\u9054 7 \u5929\uff0c\u6216\u5132\u5b58\u65bc -20\u00b0C \u53ef\u9054 3 \u500b\u6708\u3002<\/p>\n<\/li>\n

    • \n

      \u7d44\u7e54\u6a23\u672c<\/strong>:\u4f7f\u7528 \u2264100 mg \u7684\u809d\u81df\u6216\u5176\u4ed6\u7d44\u7e54\uff0c\u7528 200-500 \u03bcL \u7121\u83cc\u6c34\u5747\u8cea\u5316\u3002<\/p>\n<\/li>\n<\/ul>\n

      \n

      \u8acb\u6ce8\u610f\uff1a<\/strong> \u9700\u8981\u6839\u64da\u4ee5\u4e0b\u689d\u4ef6\u9032\u884c\u9810\u5148\u589e\u88dc GB 4789.4-2016<\/em> \u6a19\u6e96\u6216\u540c\u7b49\u6a19\u6e96\u3002\u5efa\u8b70\u5728 BPW \u8089\u6e6f\uff081:10 w\/v\uff09\u4e2d\u6fc3\u7e2e\uff0c\u7136\u5f8c\u5728 36 \u00b1 1\u00b0C \u4e0b\u57f9\u990a 8-18 \u5c0f\u6642\u3002<\/p>\n<\/blockquote>\n

      \u8acb\u4f7f\u7528 DN\/RNA \u62bd\u63d0\u5957\u4ef6 (Cat. No. DP201)<\/strong> \u6216\u540c\u7b49\u65b9\u6cd5\u63d0\u53d6\u6838\u9178\u3002\u8403\u53d6\u7684 DNA \u61c9\u7f6e\u65bc\u51b0\u4e0a\uff0c\u4e26\u7acb\u5373\u9032\u884c\u6e2c\u8a66\uff084\u00b0C \u4e0b \u22647 \u5929\u6216 -20\u00b0C \u4e0b \u22646 \u500b\u6708\uff09\u3002<\/p>\n

      \n

      PCR \u5354\u5b9a<\/strong><\/h3>\n
        \n
      1. \n

        \u53cd\u61c9\u6df7\u5408\u7269\u88fd\u5099\uff1a<\/strong><\/p>\n

          \n
        • \n

          \u5728\u5ba4\u6eab\u4e0b\u89e3\u51cd\u6240\u6709\u8a66\u5291\uff0c\u4e26\u7c21\u55ae\u96e2\u5fc3\u3002<\/p>\n<\/li>\n

        • \n

          \u5c07 23 \u03bcL \u7684\u6c99\u9580\u6c0f\u83cc PCR \u6df7\u5408\u7269\u5206\u9001\u81f3\u6bcf\u500b PCR \u7ba1\u4e2d\u3002<\/p>\n<\/li>\n

        • \n

          \u5728\u6bcf\u500b\u8a66\u7ba1\u4e2d\u5206\u5225\u52a0\u5165 2 \u03bcL \u6a23\u54c1 DNA\u3001\u967d\u6027\u5c0d\u7167\u6216\u9670\u6027\u5c0d\u7167\u3002<\/p>\n<\/li>\n

        • \n

          \u6700\u7d42\u5377\uff1a \u6bcf\u500b\u53cd\u61c9 25 \u03bcL<\/strong>.<\/p>\n<\/li>\n<\/ul>\n<\/li>\n

        • \n

          PCR \u7a0b\u5f0f\uff1a<\/strong><\/p>\n

            \n
          • \n

            UNG \u8655\u7406\uff1a50\u00b0C 2 \u5206\u9418<\/p>\n<\/li>\n

          • \n

            \u521d\u59cb\u8b8a\u6027\uff1a 95\u00b0C 5 \u5206\u9418<\/p>\n<\/li>\n

          • \n

            40 \u5faa\u74b0\uff1a<\/p>\n

              \n
            • \n

              \u8b8a\u6027\uff1a95\u00b0C 10 \u79d2<\/p>\n<\/li>\n

            • \n

              \u9000\u706b\/\u5ef6\u9577\uff1a60\u00b0C 35 \u79d2\uff08\u6536\u96c6 FAM \u8a0a\u865f\uff09<\/p>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n

            • \n

              \u87a2\u5149\u901a\u9053\uff1a<\/strong><\/p>\n

                \n
              • \n

                FAM (\u9069\u7528\u65bc ABI \u7cfb\u5217\u53ca\u5176\u4ed6 qPCR \u5100\u5668)<\/p>\n<\/li>\n

              • \n

                \u82e5\u5100\u5668\u9700\u8981\uff0c\u52a0\u5165 ROX \u53c3\u8003\u67d3\u6599\u3002<\/p>\n<\/li>\n<\/ul>\n<\/li>\n<\/ol>\n

                \n

                \u7d50\u679c\u8a6e\u91cb<\/strong><\/h3>\n
                \n
                \n\n\n\n\n\n\n\n
                \u72c0\u6cc1<\/th>\n\u8a6e\u91cb<\/th>\n<\/tr>\n<\/thead>\n
                Ct \u2264 35 + S \u578b\u66f2\u7dda<\/strong><\/td>\n\u6c99\u9580\u6c0f\u83cc DNA \u967d\u6027<\/td>\n<\/tr>\n
                Ct > 35 \u548c < 40<\/strong><\/td>\n\u53ef\u7591 - \u5efa\u8b70\u91cd\u8907\u6e2c\u8a66<\/td>\n<\/tr>\n
                Ct \u2265 40 \u6216\u672a\u78ba\u5b9a<\/strong><\/td>\n\u9670\u6027 - \u672a\u6aa2\u6e2c\u5230\u6c99\u9580\u6c0f\u83cc<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
                \n
                <\/div>\n<\/div>\n<\/div>\n<\/div>\n
                \n

                \u6ce8\u610f\uff1a\u53ef\u7591\u7d50\u679c\u53ef\u80fd\u7531\u6291\u5236\u5291\uff08\u5982\u9152\u7cbe\u3001\u6297\u51dd\u5291\uff09\u6216 DNA \u7522\u91cf\u4f4e\u5f15\u8d77\u3002\u5982\u679c\u6392\u9664\u4e86\u6c23\u6eb6\u81a0\u6c61\u67d3\uff0c\u4e14\u518d\u6b21\u6aa2\u6e2c\u6642 Ct <35\uff0c\u5247\u8a72\u7d50\u679c\u88ab\u8996\u70ba\u967d\u6027\u3002<\/em><\/p>\n<\/blockquote>\n

                \n

                \u54c1\u8cea\u63a7\u5236\u6a19\u6e96<\/strong><\/h3>\n
                  \n
                • \n

                  \u967d\u6027\u5c0d\u7167\uff1a<\/strong> FAM Ct <28\uff0c\u64f4\u589e\u66f2\u7dda\u5448 S \u578b<\/p>\n<\/li>\n

                • \n

                  \u9670\u6027\u5c0d\u7167\uff1a<\/strong> \u7121 Ct \u6216 Ct \u226540 \u4e14\u7121 S \u578b\u66f2\u7dda<\/p>\n<\/li>\n

                • \n

                  \u5426\u5247\uff0c\u7d50\u679c\u8996\u70ba\u7121\u6548\uff0c\u9700\u8981\u91cd\u65b0\u6e2c\u8a66\u3002<\/p>\n<\/li>\n<\/ul>\n

                  \n

                  \u9810\u9632\u63aa\u65bd<\/strong><\/h3>\n
                    \n
                  1. \n

                    \u5206\u5340\u5de5\u4f5c\u6d41\u7a0b\uff1a<\/strong> \u5728\u9810PCR\u3001\u8a66\u5291\u8a2d\u5b9a\u548c\u64f4\u5927\u5340\u57df\u4f7f\u7528\u7269\u7406\u5206\u9694\u3002\u7a7f\u6234\u9632\u8b77\u88dd\u5099\uff0c\u4e26\u5728\u4e0d\u540c\u5340\u57df\u4e4b\u9593\u66f4\u63db\u624b\u5957\u3002<\/p>\n<\/li>\n

                  2. \n

                    \u907f\u514d\u6c61\u67d3\uff1a<\/strong> \u5ef6\u9072 PCR \u5f8c\u7684\u8a66\u7ba1\u958b\u555f\u6642\u9593\uff0c\u4ee5\u907f\u514d\u7522\u751f\u5674\u9727\u3002\u5f37\u70c8\u5efa\u8b70\u5c0d\u8a2d\u5099\u9032\u884c\u7d2b\u5916\u7dda\u6216\u5316\u5b78\u6d88\u6bd2\u3002<\/p>\n<\/li>\n

                  3. \n

                    \u51cd\u878d\u5faa\u74b0\uff1a<\/strong> \u907f\u514d\u91cd\u8907\u51cd\u878d\u8a66\u5291\u3002\u4f7f\u7528\u524d\u8acb\u5b8c\u5168\u89e3\u51cd\u4e26\u8f15\u8f15\u6df7\u548c\u3002<\/p>\n<\/li>\n

                  4. \n

                    \u50c5\u4f9b\u7814\u7a76\u4f7f\u7528\uff1a<\/strong> \u672c\u5957\u4ef6\u9069\u7528\u65bc \u79d1\u5b78\u7814\u7a76<\/strong> \u50c5\u9069\u7528\u65bc\u98df\u54c1\/\u74b0\u5883\u5b89\u5168\u7be9\u6aa2\u3002\u4e0d\u53ef\u7528\u65bc\u81e8\u5e8a\u8a3a\u65b7\u3002<\/p>\n<\/li>\n<\/ol>\n

                    \n

                    \u5132\u5b58\u8207\u4fdd\u5b58\u671f\u9650<\/strong><\/h3>\n
                      \n
                    • \n

                      \u5132\u5b58\u65bc -20\u00b0C<\/strong><\/p>\n<\/li>\n

                    • \n

                      \u6709\u6548\u671f\u81f3\u5c11 12 \u500b\u6708<\/strong> \u5982\u679c\u672a\u958b\u5c01\u4e26\u59a5\u5584\u4fdd\u5b58<\/p>\n<\/li>\n

                    • \n

                      \u9632\u6b62\u5149\u7dda\u7167\u5c04\u548c\u53cd\u8986\u51cd\u878d\u5faa\u74b0<\/p>\n<\/li>\n<\/ul>","protected":false},"excerpt":{"rendered":"

                      \u76ee\u9304\u7de8\u865f\uff1aAP2409004Specifications:50 \u6e2c\u8a66\u6b21\/100 \u6e2c\u8a66\u6b21\u5132\u5b58\u6eab\u5ea6\uff1a-20\u00b0C \u7522\u54c1\u8aaa\u660e \u6c99\u9580\u6c0f\u83cc\u6838\u9178\u6aa2\u6e2c\u8a66\u5291\u76d2\u662f\u4e00\u7a2e\u5373\u6642 PCR \u6aa2\u6e2c\u65b9\u6cd5\uff0c\u5c08\u70ba\u5feb\u901f\u3001\u7279\u7570\u3001\u9748\u654f\u5730\u6aa2\u6e2c\u591a\u7a2e\u6a23\u672c\uff08\u5305\u62ec\u7cde\u4fbf\u3001\u6c34\u3001\u7d44\u7e54\u548c\u98df\u7269\uff09\u4e2d\u7684\u6c99\u9580\u6c0f\u83cc DNA \u800c\u8a2d\u8a08\u3002\u6c99\u9580\u6c0f\u83cc\u662f\u4e00\u7a2e\u4e3b\u8981\u7684\u98df\u6e90\u6027\u75c5\u539f\u83cc\uff0c\u901a\u5e38\u7d93\u7531\u53d7\u6c61\u67d3\u7684\u98df\u7269\u50b3\u64ad\u3002<\/p>","protected":false},"featured_media":398,"comment_status":"open","ping_status":"closed","template":"","meta":{"_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"default","_kad_post_title":"","_kad_post_layout":"default","_kad_post_sidebar_id":"","_kad_post_content_style":"default","_kad_post_vertical_padding":"default","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"_kad_post_classname":""},"product_brand":[126],"product_cat":[125],"product_tag":[],"class_list":["post-397","product","type-product","status-publish","has-post-thumbnail","product_brand-sanshi-biotech","product_cat-bird-dna-test","first","instock","shipping-taxable","product-type-simple","entry","content-bg","loop-entry"],"_links":{"self":[{"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/product\/397","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/comments?post=397"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/media\/398"}],"wp:attachment":[{"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/media?parent=397"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/product_brand?post=397"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/product_cat?post=397"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/product_tag?post=397"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}