{"id":405,"date":"2025-06-30T09:42:44","date_gmt":"2025-06-30T09:42:44","guid":{"rendered":"https:\/\/birdstesting.com\/?post_type=product&p=405"},"modified":"2025-06-30T09:42:45","modified_gmt":"2025-06-30T09:42:45","slug":"%e9%b4%bf%e5%ad%90%e8%85%ba%e7%97%85%e6%af%92-piadv-dna-%e6%b8%ac%e8%a9%a6%e5%a5%97%e4%bb%b6-pcr-ung-50t-100t","status":"publish","type":"product","link":"https:\/\/birdstesting.com\/zh\/%e9%b4%bf%e5%ad%90%e8%85%ba%e7%97%85%e6%af%92-piadv-dna-%e6%b8%ac%e8%a9%a6%e5%a5%97%e4%bb%b6-pcr-ung-50t-100t\/","title":{"rendered":"\u9d3f\u5b50\u817a\u75c5\u6bd2 (PiADV) DNA \u6e2c\u8a66\u5957\u4ef6 - PCR (UNG) 50T\/100T"},"content":{"rendered":"

\u9d3f\u5b50\u817a\u75c5\u6bd2 (PiADV) DNA \u6e2c\u8a66<\/a> \u5957\u4ef6 - PCR \u87a2\u5149\u63a2\u91dd\u6cd5 (UNG)<\/strong>
\u76ee\u9304\u7de8\u865f\uff1a<\/strong> AP2409005\u898f\u683c\uff1a<\/strong> 50T \/ 100T\u5132\u5b58\uff1a<\/strong> -20\u00b0C<\/p>\n

\n

\u7522\u54c1\u7e3d\u89bd\uff1a<\/strong><\/h3>\n

\u7684 \u9d3f\u5b50\u817a\u75c5\u6bd2 (PiADV) DNA \u6aa2\u6e2c\u8a66\u5291\u76d2<\/strong> \u662f\u4e00\u7a2e\u9748\u654f\u4e14\u7279\u7570\u7684 qPCR \u8a3a\u65b7\u5de5\u5177\uff0c\u5c08\u70ba\u6aa2\u6e2c PiADV<\/strong>PiADV \u662f\u4e00\u7a2e\u9ad8\u5ea6\u50b3\u67d3\u6027\u7684\u75c5\u6bd2\uff0c\u6703\u611f\u67d3\u9d3f\u5b50\u3001\u9e1a\u9d61\u548c\u5176\u4ed6\u9ce5\u985e\u3002PiADV \u662f\u5c0e\u81f4 \u5e7c\u9d3f\u6025\u6027\u8179\u7009<\/strong> \u4e26\u900f\u904e\u5169\u8005\u8fc5\u901f\u64f4\u6563 \u5782\u76f4\u50b3\u64ad<\/strong> \u548c \u6c34\u5e73\u63a5\u89f8<\/strong>.<\/p>\n

\u76ee\u524d\u5c1a\u7121\u6709\u6548\u7684\u6297\u75c5\u6bd2\u6cbb\u7642\u65b9\u6cd5\u3002\u56e0\u6b64\uff0c\u4f7f\u7528\u6b64 PiADV \u6838\u9178\u6aa2\u6e2c\u8a66\u5291\u76d2\u9032\u884c\u65e9\u671f\u6e96\u78ba\u6aa2\u6e2c\uff0c\u5c0d\u65bc\u53ca\u6642\u7ba1\u7406\u75be\u75c5\u548c\u6e1b\u5c11\u75c5\u6bd2\u5728\u9ce5\u985e\u65cf\u7fa4\u4e2d\u7684\u50b3\u64ad\u81f3\u95dc\u91cd\u8981\u3002<\/p>\n

\u672c\u8a66\u5291\u76d2\u4f7f\u7528\u91dd\u5c0d\u4fdd\u5b88\u7684 PiADV \u57fa\u56e0\u5e8f\u5217\u7684\u6700\u4f73\u5316\u5f15\u7269\u548c\u87a2\u5149\u63a2\u91dd\u3002\u672c\u8a66\u5291\u76d2\u5229\u7528\u91dd\u5c0d\u4fdd\u5b88\u7684 PiADV \u57fa\u56e0\u5e8f\u5217\u7684\u6700\u4f73\u5f15\u7269\u548c\u87a2\u5149\u63a2\u91dd\uff0c\u53ef\u5f9e \u53e3\u8154\/\u53e3\u8154\u62ed\u5b50<\/strong>, \u5168\u8840<\/strong>\u4ee5\u53ca \u7d44\u7e54\u6a23\u672c<\/strong>.<\/p>\n

\n

\u4e3b\u8981\u529f\u80fd\uff1a<\/strong><\/h3>\n
    \n
  • \n

    \u2705 \u5feb\u901f\u53ef\u9760\u5730\u6aa2\u6e2c \u9d3f\u5b50\u817a\u75c5\u6bd2 (PiADV)<\/strong><\/p>\n<\/li>\n

  • \n

    \u2705 \u9069\u7528\u65bc\u53e3\u8154\u62ed\u5b50\u3001\u6cc4\u6b96\u8154\u62ed\u5b50\u3001\u8840\u6db2 (EDTA) \u548c\u5668\u5b98\u7d44\u7e54<\/p>\n<\/li>\n

  • \n

    \u5df2\u9a57\u8b49 \u5b9a\u91cf\u5373\u6642 PCR (qPCR)<\/strong> \u4f7f\u7528 FAM \u983b\u9053<\/p>\n<\/li>\n

  • \n

    \u5167\u90e8 \u5305\u62ec\u967d\u6027\u548c\u9670\u6027\u5c0d\u7167<\/strong><\/p>\n<\/li>\n

  • \n

    \u2705 \u53ef\u7528\u65bc 50 \u6e2c\u8a66\u548c 100 \u6e2c\u8a66\u5957\u4ef6<\/strong><\/p>\n<\/li>\n

  • \n

    \u2705 \u4fdd\u8cea\u671f \u2265 12 \u500b\u6708\uff0c\u5132\u5b58\u65bc -20\u00b0C \u6642<\/p>\n<\/li>\n

  • \n

    \u2705 \u9ce5\u985e\u8a3a\u65b7\u5be6\u9a57\u5ba4\u3001\u7378\u91ab\u3001\u98fc\u990a\u54e1\u548c\u8cfd\u9d3f\u611b\u597d\u8005\u7684\u7406\u60f3\u9078\u64c7<\/p>\n<\/li>\n<\/ul>\n

    \n

    \u5957\u4ef6\u5167\u5bb9\uff1a<\/strong><\/h3>\n
    \n
    \n\n\n\n\n\n\n\n
    \u7d44\u4ef6<\/th>\n50T<\/th>\n100T<\/th>\n<\/tr>\n<\/thead>\n
    PiADV \u6df7\u5408\u8a66\u5291<\/td>\n1150 \u00b5L<\/td>\n2 \u00d7 1150 \u00b5L<\/td>\n<\/tr>\n
    \u967d\u6027\u5c0d\u7167<\/td>\n100 \u00b5L<\/td>\n100 \u00b5L<\/td>\n<\/tr>\n
    \u9670\u6027\u5c0d\u7167<\/td>\n100 \u00b5L<\/td>\n100 \u00b5L<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
    \n
    <\/div>\n<\/div>\n<\/div>\n<\/div>\n
    \n

    \u6a23\u54c1\u985e\u578b\u8207\u8981\u6c42\uff1a<\/strong><\/h3>\n
      \n
    • \n

      \u68c9\u82b1\u68d2\uff1a<\/strong> \u7121\u83cc\u53e3\u8154\u6216\u6cc4\u6b96\u8154\u62ed\u5b50\uff0c\u5132\u5b58\u65bc 2-8\u00b0C (\u226424h) \u6216 -20\u00b0C (\u82e5\u5ef6\u9072)<\/p>\n<\/li>\n

    • \n

      \u8840\u6db2\uff1a<\/strong> EDTA \u8655\u7406\u904e\u7684\u5168\u8840\uff08\u975e\u809d\u7d20\uff09\uff0c\u65b0\u9bae\u6216\u65bc 2-8\u00b0C \u5132\u5b58 \u22647 \u5929\u6216\u65bc -20\u00b0C \u5132\u5b58 \u22643 \u500b\u6708<\/p>\n<\/li>\n

    • \n

      \u7d44\u7e54\uff1a<\/strong> \u2264\u5728 200-500 \u00b5L \u7121\u83cc\u6c34\u4e2d\u5747\u8cea\u7684 100 \u6beb\u514b\u65b0\u9bae\u809d\u81df<\/p>\n<\/li>\n<\/ul>\n

      \u6838\u9178\u61c9\u4f7f\u7528 DP202 \u6216\u540c\u7b49\u7d1a\u7684\u8403\u53d6\u5957\u4ef6<\/strong>.\u8403\u53d6\u7684 DNA \u53ef\u5728 4\u00b0C \u5132\u5b58 \u22647 \u5929\uff0c\u6216\u5728 -20\u00b0C \u5132\u5b58 \u22646 \u500b\u6708\u3002<\/p>\n

      \n

      qPCR \u53cd\u61c9\u8a2d\u5b9a\uff1a<\/strong><\/h3>\n
        \n
      1. \n

        \u5b8c\u5168\u89e3\u51cd\u8a66\u5291\u4e26\u7c21\u77ed\u96e2\u5fc3\u3002<\/p>\n<\/li>\n

      2. \n

        \u70ba\u6bcf\u500b\u6e2c\u8a66\u6a23\u672c + 2 \u500b\u5c0d\u7167\uff08\u967d\u6027\u548c\u9670\u6027\uff09\uff0c\u6e96\u5099\u53cd\u61c9\u7ba1\uff1a<\/p>\n

          \n
        • \n

          \u65b0\u589e 23 \u00b5L PiADV \u6aa2\u6e2c\u6df7\u5408\u6db2<\/strong><\/p>\n<\/li>\n

        • \n

          \u65b0\u589e 2 \u00b5L DNA \u6a23\u54c1<\/strong>, \u6b63\u9762<\/strong>\u00a0\u6216 \u9670\u6027\u5c0d\u7167<\/strong><\/p>\n<\/li>\n

        • \n

          \u7e3d\u5bb9\u91cf\uff1a 25 \u00b5L<\/strong> \u6bcf\u7ba1<\/p>\n<\/li>\n<\/ul>\n<\/li>\n<\/ol>\n

          \n

          PCR \u5faa\u74b0\u53c3\u6578\uff1a<\/strong><\/h3>\n
          \n
          \n\n\n\n\n\n\n\n\n
          \u6b65\u9a5f<\/th>\n\u6eab\u5ea6<\/th>\n\u6642\u9593<\/th>\n<\/tr>\n<\/thead>\n
          UNG \u6de8\u5316<\/td>\n50\u00b0C<\/td>\n2 \u5206\u9418<\/td>\n<\/tr>\n
          \u9810\u8b8a\u6027<\/td>\n95\u00b0C<\/td>\n5 \u5206\u9418<\/td>\n<\/tr>\n
          \u653e\u5927 (\u00d740)<\/td>\n95\u00b0C<\/td>\n10 \u79d2<\/td>\n<\/tr>\n
          <\/td>\n60\u00b0C<\/td>\n35 \u79d2 (FAM)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
          \n
          <\/div>\n<\/div>\n<\/div>\n<\/div>\n
          \n

          \u8207 ABI \u53ca\u5176\u4ed6\u5373\u6642 PCR \u7cfb\u7d71\u76f8\u5bb9\u3002\u5982\u6709\u9700\u8981\uff0c\u53ef\u9078\u8cfc ROX \u67d3\u6599\u3002<\/p>\n<\/blockquote>\n

          \n

          \u7d50\u679c\u95e1\u91cb\uff1a<\/strong><\/h3>\n
            \n
          • \n

            \u6b63\u9762\uff1a<\/strong> Ct \u2264 33\uff0c\u5e36\u6709 \"S \"\u66f2\u7dda<\/p>\n<\/li>\n

          • \n

            \u5acc\u7591\u72af<\/strong> Ct 33-38 - \u5efa\u8b70\u91cd\u65b0\u6e2c\u8a66<\/p>\n<\/li>\n

          • \n

            \u8ca0\u9762\uff1a<\/strong> Ct \u2265 38 \u6216\u7121\u64f4\u589e<\/p>\n<\/li>\n<\/ul>\n

            \u63a7\u5236\u9805\u5fc5\u9808\u7b26\u5408\u6709\u6548\u6027\u6a19\u6e96\uff1a<\/strong><\/p>\n

              \n
            • \n

              \u967d\u6027\u5c0d\u7167 Ct < 28<\/p>\n<\/li>\n

            • \n

              \u9670\u6027\u5c0d\u7167\uff1aCt \u2265 38 \u6216\u672a\u6aa2\u51fa<\/p>\n<\/li>\n<\/ul>\n

              \n

              \u91cd\u8981\u6ce8\u610f\u4e8b\u9805\uff1a<\/strong><\/h3>\n
                \n
              1. \n

                \u56b4\u683c\u5206\u9694\u6a23\u54c1\u6e96\u5099\u5340\u3001\u8a66\u5291\u8655\u7406\u5340\u548c\u64f4\u5927\u5340\uff0c\u4ee5\u9632\u6b62\u6c61\u67d3<\/p>\n<\/li>\n

              2. \n

                \u7a7f\u6234\u624b\u5957\u548c\u5be6\u9a57\u5ba4\u670d\u88dd\uff1b\u5b9a\u671f\u66f4\u63db<\/p>\n<\/li>\n

              3. \n

                \u904b\u884c\u5f8c\u8acb\u52ff\u7acb\u5373\u6253\u958b PCR \u7ba1 - \u5f85\u5176\u51b7\u537b<\/p>\n<\/li>\n

              4. \n

                \u907f\u514d\u91cd\u8907\u51cd\u878d\u8a66\u5291<\/p>\n<\/li>\n

              5. \n

                \u50c5\u4f9b\u7814\u7a76\u4f7f\u7528 - \u4e0d\u9069\u7528\u65bc\u81e8\u5e8a\u8a3a\u65b7<\/strong><\/p>\n<\/li>\n

              6. \n

                \u8a66\u5291\u8981\u907f\u5149\u4e26\u6309\u6307\u793a\u5132\u5b58<\/p>\n<\/li>\n<\/ol>\n

                \n

                \ud83d\uded2 \u5b89\u5168\u3001\u53ef\u9760\u5730\u6aa2\u6e2c\u60a8\u7684\u9d3f\u5b50\u6216\u79bd\u9ce5\u7a2e\u985e\u7684 PiADV<\/strong>.\u975e\u5e38\u9069\u5408\u75be\u75c5\u7be9\u6aa2\u3001\u990a\u6b96\u8a2d\u65bd\u5065\u5eb7\u76e3\u63a7\u548c\u79d1\u5b78\u7814\u7a76\u3002<\/p>","protected":false},"excerpt":{"rendered":"

                \u9d3f\u5b50\u817a\u75c5\u6bd2 (PiADV) DNA \u6aa2\u6e2c\u8a66\u5291\u76d2 - PCR \u87a2\u5149\u63a2\u91dd\u6cd5 (UNG)Catalog No.: AP2409005 Specification\uff1a50T \/ 100T \u5132\u5b58\u6eab\u5ea6\uff1a-20\u00b0C \u7522\u54c1\u6982\u8ff0\uff1a\u9d3f\u5b50\u817a\u75c5\u6bd2 (PiADV) DNA \u6aa2\u6e2c\u8a66\u5291\u76d2\u662f\u4e00\u7a2e\u9748\u654f\u4e14\u7279\u7570\u7684 qPCR \u8a3a\u65b7\u5de5\u5177\uff0c\u5c08\u70ba\u6aa2\u6e2c PiADV \u800c\u8a2d\u8a08\uff0cPiADV \u662f\u4e00\u7a2e\u611f\u67d3\u9d3f\u5b50\u3001\u9e1a\u9d61\u548c\u5176\u4ed6\u9ce5\u985e\u7684\u9ad8\u50b3\u67d3\u6027\u75c5\u6bd2\u3002PiADV \u662f\u4e00\u7a2e...<\/p>","protected":false},"featured_media":406,"comment_status":"open","ping_status":"closed","template":"","meta":{"_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"default","_kad_post_title":"","_kad_post_layout":"default","_kad_post_sidebar_id":"","_kad_post_content_style":"default","_kad_post_vertical_padding":"default","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"_kad_post_classname":""},"product_brand":[],"product_cat":[125],"product_tag":[],"class_list":["post-405","product","type-product","status-publish","has-post-thumbnail","product_cat-bird-dna-test","first","instock","shipping-taxable","product-type-simple","entry","content-bg","loop-entry"],"_links":{"self":[{"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/product\/405","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/comments?post=405"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/media\/406"}],"wp:attachment":[{"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/media?parent=405"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/product_brand?post=405"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/product_cat?post=405"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/product_tag?post=405"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}