{"id":407,"date":"2025-06-30T10:07:55","date_gmt":"2025-06-30T10:07:55","guid":{"rendered":"https:\/\/birdstesting.com\/?post_type=product&p=407"},"modified":"2025-06-30T10:07:56","modified_gmt":"2025-06-30T10:07:56","slug":"%e7%a6%bd%e6%b5%81%e6%84%9f%e6%b8%ac%e8%a9%a6%e5%a5%97%e4%bb%b6","status":"publish","type":"product","link":"https:\/\/birdstesting.com\/zh\/%e7%a6%bd%e6%b5%81%e6%84%9f%e6%b8%ac%e8%a9%a6%e5%a5%97%e4%bb%b6\/","title":{"rendered":"\u79bd\u6d41\u611f\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08H1N1 \u4e9e\u578b\u75c5\u6bd2\uff09\u5373\u6642 PCR \u6aa2\u6e2c\u8a66\u5291\u76d2"},"content":{"rendered":"

\u7522\u54c1\u540d\u7a31 (EN)\uff1a <\/strong> \u79bd\u6d41\u611f \u6e2c\u8a66\u5957\u4ef6<\/a>
\u5305\u88dd\u5c3a\u5bf8\uff1a<\/strong> 50 \u9805\u6e2c\u8a66
\u50f9\u683c\uff1a<\/strong> \u806f\u7d61\u6211\u5011\u7684\u92b7\u552e\u4eba\u54e1
\u5132\u5b58\uff1a<\/strong> \u4f4e\u6eab\u904b\u8f38\uff1b\u5132\u5b58\u65bc -20\u00b0C<\/strong>\uff0c\u6709\u6548\u671f\u70ba 12 \u500b\u6708
\u904b\u9001\u5f9e\uff1a<\/strong> \u4e2d\u570b\u4e0a\u6d77
\u50c5\u4f9b\u7814\u7a76\u4f7f\u7528<\/strong><\/p>\n

\n

\u79bd\u6d41\u611f\u6aa2\u6e2c\u5957\u4ef6\u6982\u8ff0<\/strong><\/h2>\n

\u672c\u79bd\u6d41\u611f\u6aa2\u6e2c\u5957\u4ef6\u5c08\u9580\u8a2d\u8a08\u7528\u65bc \u7532\u578b\u6d41\u611f\u75c5\u6bd2 H1N1 \u4e9e\u578b RNA \u7684\u5b9a\u6027\u548c\u5b9a\u91cf\u6aa2\u6e2c<\/strong> \u4f7f\u7528 \u63a2\u91dd\u5f0f\u87a2\u5149 qRT-PCR \u6280\u8853<\/strong>.\u8a72\u8a66\u5291\u76d2\u91dd\u5c0d H1N1 \u4e9e\u578b\u7684\u4fdd\u5b88\u907a\u50b3\u5340\u57df\uff0c\u5728\u6aa2\u6e2c\u751f\u7269\u548c\u74b0\u5883\u6a23\u672c\u4e2d\u7684\u79bd\u6d41\u611f A \u578b\u75c5\u6bd2\u6642\u5177\u6709\u9ad8\u5ea6\u7279\u7570\u6027\u548c\u9748\u654f\u5ea6\u3002<\/p>\n

\u8207\u4ee5 SYBR Green \u67d3\u6599\u70ba\u57fa\u790e\u7684 PCR \u4e0d\u540c\uff0c\u6b64 \u4ee5 TaqMan \u63a2\u91dd\u70ba\u57fa\u790e\u7684\u7cfb\u7d71<\/strong> \u4f7f\u7528\u87a2\u5149\u6a19\u8a18\u63a2\u91dd\uff0c\u53ea\u6709\u5728\u96dc\u4ea4\u548c\u64f4\u589e\u904e\u7a0b\u4e2d\u88c2\u89e3\u6642\u624d\u6703\u767c\u51fa\u8a0a\u865f\uff0c\u78ba\u4fdd \u5353\u8d8a\u7684\u7279\u7570\u6027<\/strong> \u4e26\u964d\u4f4e\u5047\u967d\u6027\u7387\u3002<\/p>\n

\n

\u4e3b\u8981\u529f\u80fd<\/strong><\/h2>\n

\u2705 \u9ad8\u5ea6\u5177\u9ad4\u548c\u9748\u654f<\/strong> - \u6839\u64da H1N1 \u57fa\u56e0\u7d44\u7684\u9ad8\u5ea6\u4fdd\u5b88\u5340\u57df\u8a2d\u8a08\uff1b\u8207\u975e\u76ee\u6a19\u75c5\u6bd2\u7121\u4ea4\u53c9\u53cd\u61c9
\u2705 \u5373\u7528\u578b\u7cfb\u7d71<\/strong> - \u53ea\u9700\u8981\u4f7f\u7528\u8005\u63d0\u4f9b RNA \u6a21\u677f
\u2705 \u512a\u5316\u5f15\u7269\u8207\u63a2\u91dd<\/strong> - \u78ba\u4fdd\u6709\u6548\u7684\u64f4\u589e\u548c\u6aa2\u6e2c
\u2705 \u5305\u542b\u967d\u6027\u5c0d\u7167<\/strong> - \u7528\u65bc\u7d50\u679c\u9a57\u8b49\u7684\u5408\u6210\u975e\u50b3\u67d3\u6027 DNA
\u2705 \u5b9a\u91cf\u8207\u5b9a\u6027\u61c9\u7528<\/strong> - \u9069\u7528\u65bc\u7814\u7a76\u3001\u76e3\u63a7\u548c\u7be9\u6aa2\u7528\u9014
\u2705 \u8207\u4e3b\u8981 qPCR \u5100\u5668\u76f8\u5bb9<\/strong> - FAM \u901a\u9053\u4e0a\u7684\u87a2\u5149\u5075\u6e2c<\/p>\n

\n

\u5957\u4ef6\u5167\u5bb9\uff0850 \u9805\u6e2c\u8a66\uff09<\/strong><\/h2>\n
\n
\n\n\n\n\n\n\n\n\n\n\n\n\n
\u5143\u4ef6\u8aaa\u660e<\/th>\n\u4ee3\u78bc<\/th>\n\u5bb9\u91cf\u8207\u84cb\u5b50\u984f\u8272<\/th>\n<\/tr>\n<\/thead>\n
qRT-PCR \u7de9\u885d\u6db2\uff08\u63a2\u91dd\u7cfb\u7d71\uff09<\/td>\nA<\/td>\n500 \u00b5L<\/td>\n<\/tr>\n
qRT-PCR \u8907\u5408\u9176\u6df7\u5408\u7269<\/td>\nB<\/td>\n100 \u00b5L<\/td>\n<\/tr>\n
PCR \u6a21\u677f\u7a00\u91cb\u7de9\u885d\u6db2<\/td>\nC<\/td>\n1 mL<\/td>\n<\/tr>\n
\u9069\u7528\u65bc H1N1 \u7684 qRT-PCR \u5f15\u7269\u6df7\u5408\u7269<\/td>\nD<\/td>\n100 \u00b5L<\/td>\n<\/tr>\n
H1N1 \u4e9e\u578b\u7684\u63a2\u91dd<\/td>\nE<\/td>\n50 \u00b5L<\/td>\n<\/tr>\n
\u967d\u6027\u5c0d\u7167 DNA (1\u00d710\u2078 copies\/\u03bcL)<\/td>\nF<\/td>\n50 \u00b5L<\/td>\n<\/tr>\n
RNA \u91cb\u653e\u8a66\u5291\uff08\u8a66\u7528\uff0c\u514d\u62bd\u53d6\u6cd5\uff09<\/td>\nG<\/td>\n20 \u6e2c\u8a66<\/td>\n<\/tr>\n
\u4f7f\u7528\u624b\u518a<\/td>\nH<\/td>\n1 \u4efd<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
\n
<\/div>\n<\/div>\n<\/div>\n<\/div>\n
\n

\u5982\u4f55\u904b\u4f5c<\/strong><\/h3>\n

\u672c\u8a66\u5291\u76d2\u900f\u904e\u4e00\u6b65\u5f0f\u9006\u8f49\u9304 PCR \u53cd\u61c9\u6aa2\u6e2c\u75c5\u6bd2 RNA\u3002\u5728\u64f4\u589e\u904e\u7a0b\u4e2d\uff0c Taq \u805a\u5408\u9176\u88c2\u89e3\u96d9\u6a19\u793a\u63a2\u91dd<\/strong>\uff0c\u5c07\u87a2\u5149\u5718\u8207\u6dec\u706b\u5291\u5206\u96e2\uff0c\u7522\u751f\u8207\u76ee\u6a19 RNA \u542b\u91cf\u6210\u6b63\u6bd4\u7684\u87a2\u5149\u4fe1\u865f\u3002<\/p>\n

\u6bcf\u500b\u9031\u671f\u90fd\u6703\u6e2c\u91cf\u87a2\u5149\uff0c\u8b93 \u5373\u6642\u76e3\u63a7<\/strong> \u7684 PCR \u904e\u7a0b\u3002<\/p>\n

\n

\u5075\u6e2c\u539f\u7406<\/strong><\/h2>\n
    \n
  • \n

    Ct \u503c\uff08\u9031\u671f\u81e8\u754c\u503c\uff09\uff1a<\/strong> \u87a2\u5149\u8d85\u904e\u81e8\u754c\u503c\u6240\u9700\u7684 PCR \u9031\u671f\u6578\u3002\u8f03\u4f4e\u7684 Ct \u8868\u793a\u8f03\u9ad8\u7684\u521d\u59cb\u76ee\u6a19\u91cf\u3002<\/p>\n<\/li>\n

  • \n

    \u95be\u503c\u8a2d\u5b9a\uff1a<\/strong> \u57fa\u65bc\u6700\u521d 3-15 \u500b\u9031\u671f\uff08\u80cc\u666f\uff09\u87a2\u5149\u7684 10 \u500d\u6a19\u6e96\u504f\u5dee\u3002<\/p>\n<\/li>\n<\/ul>\n

    \n

    \u7e3e\u6548\u8207\u622a\u6b62\u6a19\u6e96<\/strong><\/h3>\n
    \n
    \n\n\n\n\n\n\n\n
    \u7d50\u679c\u985e\u578b<\/th>\n\u6a19\u6e96<\/th>\n<\/tr>\n<\/thead>\n
    \u6b63\u9762<\/strong><\/td>\nCt \u2264 35\uff0c\u5177\u6709\u5178\u578b\u7684 S \u578b\u64f4\u589e\u66f2\u7dda<\/td>\n<\/tr>\n
    \u7591\u4f3c\u967d\u6027<\/strong><\/td>\nCt 35-40 \u2192 \u5efa\u8b70\u91cd\u8907\u6e2c\u8a66<\/td>\n<\/tr>\n
    \u9670\u6027<\/strong><\/td>\nCt \u2265 40 \u6216\u7121 Ct + \u7121\u64f4\u589e\u66f2\u7dda<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
    \n
    <\/div>\n<\/div>\n<\/div>\n<\/div>\n

    \u967d\u6027\u548c\u9670\u6027\u5c0d\u7167\u5fc5\u9808\u901a\u904e\u54c1\u8cea\u6aa2\u67e5\u3002\u9670\u6027\u5c0d\u7167 Ct \u226540\uff0c\u967d\u6027\u5c0d\u7167 Ct \u226430\uff0c\u6307\u6578\u64f4\u589e\u3002<\/p>\n

    \n

    \u53cd\u61c9\u898f\u7a0b<\/strong><\/h2>\n

    \u53cd\u8f49\u9304 + PCR (\u6bcf\u500b\u53cd\u61c9 20 \u03bcL)<\/strong><\/h4>\n
    \n
    \n\n\n\n\n\n\n\n\n\n
    \u8a66\u5291<\/th>\n\u9ad4\u7a4d<\/th>\n<\/tr>\n<\/thead>\n
    qRT-PCR \u7de9\u885d\u6db2<\/td>\n10 \u03bcL<\/td>\n<\/tr>\n
    \u6df7\u5408\u9175\u7d20<\/td>\n2 \u03bcL<\/td>\n<\/tr>\n
    \u6df7\u5408\u5e95\u6f06<\/td>\n2 \u03bcL<\/td>\n<\/tr>\n
    \u63a2\u91dd<\/td>\n1 \u03bcL<\/td>\n<\/tr>\n
    RNA \u6a21\u677f\u6216\u5c0d\u7167<\/td>\n5 \u03bcL<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
    \n
    <\/div>\n<\/div>\n<\/div>\n<\/div>\n
      \n
    • \n

      \u7e3d\u5bb9\u91cf\uff1a<\/strong> 20 \u03bcL<\/p>\n<\/li>\n

    • \n

      \u71b1\u5faa\u74b0\u689d\u4ef6\uff1a<\/strong><\/p>\n<\/li>\n<\/ul>\n

      \n
      \n\n\n\n\n\n\n\n\n\n
      \u6b65\u9a5f<\/th>\n\u6eab\u5ea6<\/th>\n\u6642\u9593<\/th>\n<\/tr>\n<\/thead>\n
      \u53cd\u5411\u8f49\u9304<\/td>\n50\u00b0C<\/td>\n30 \u5206\u9418<\/td>\n<\/tr>\n
      \u521d\u59cb\u8b8a\u6027<\/td>\n94\u00b0C<\/td>\n10 \u5206\u9418<\/td>\n<\/tr>\n
      PCR \u5faa\u74b0 \u00d7 40<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
      - \u8b8a\u6027<\/td>\n94\u00b0C<\/td>\n15 \u79d2<\/td>\n<\/tr>\n
      - \u9000\u706b\/\u5ef6\u9577<\/td>\n60\u00b0C<\/td>\n1 \u5206\u9418 (FAM)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
      \n
      <\/div>\n<\/div>\n<\/div>\n<\/div>\n
      \n

      \u5b9a\u91cf\uff08\u53ef\u9078\uff09<\/strong><\/h3>\n

      \u6e96\u5099\u4e00\u4efd \u6a19\u6e96\u66f2\u7dda<\/strong> \u4f7f\u7528\u5e8f\u5217 10 \u500d\u7a00\u91cb (10\u00b2-10\u2077 copies\/\u03bcL) \u7684\u5408\u6210 DNA \u5c0d\u7167\u3002\u5c07 Ct \u503c\u5c0d\u7167 log(\u6fc3\u5ea6)\u4f5c\u5716\uff0c\u4ee5\u5167\u63d2\u672a\u77e5\u6a23\u672c\u4e2d\u7684 RNA \u8907\u672c\u6578\u3002<\/p>\n

      \n

      \u8207 SYBR Green \u76f8\u6bd4\uff0c\u63a2\u91dd\u5f0f PCR \u7684\u512a\u52e2\uff1a<\/strong><\/h2>\n
      \n
      \n\n\n\n\n\n\n\n\n
      \u7279\u9ede<\/th>\nSYBR \u7da0<\/th>\n\u63a2\u91dd\u578b (\u672c\u5957\u4ef6)<\/th>\n<\/tr>\n<\/thead>\n
      \u7279\u7570\u6027<\/td>\n\u7d50\u5408\u6240\u6709 dsDNA<\/td>\n\u53ea\u8207\u7279\u5b9a\u7684\u76ee\u6a19\u5e8f\u5217\u7d50\u5408<\/td>\n<\/tr>\n
      \u8aa4\u5224\u98a8\u96aa<\/td>\n\u66f4\u9ad8<\/td>\n\u8f03\u4f4e<\/td>\n<\/tr>\n
      \u6210\u672c<\/td>\n\u8f03\u4f4e<\/td>\n\u8f03\u9ad8\uff08\u4f46\u66f4\u53ef\u9760\uff09<\/td>\n<\/tr>\n
      \u6613\u7528\u6027<\/td>\n\u66f4\u7c21\u55ae<\/td>\n\u9700\u8981\u63a2\u91dd\u8a2d\u8a08<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
      \n
      <\/div>\n<\/div>\n<\/div>\n<\/div>\n
      \n

      \u9810\u671f\u7528\u9014<\/strong><\/h3>\n

      \u672c\u7522\u54c1\u70ba \u50c5\u4f9b\u7814\u7a76\u7528\u9014<\/strong>.\u5b83\u672a\u88ab\u6279\u51c6\u7528\u65bc \u81e8\u5e8a\u8a3a\u65b7<\/strong> \u6216\u6cbb\u7642\u7a0b\u5e8f\u3002<\/p>","protected":false},"excerpt":{"rendered":"

      \u7522\u54c1\u540d\u7a31 (EN)\uff1a\u79bd\u6d41\u611f\u6e2c\u8a66\u5957\u4ef6\u5305\u88dd\u898f\u683c: 50 \u6e2c\u8a66\u50f9\u683c\uff1a\u8acb\u806f\u7d61\u6211\u5011\u7684\u92b7\u552e\u4eba\u54e1\u5132\u5b58\u65b9\u5f0f\uff1a\u4f4e\u6eab\u904b\u8f38\uff1b-20\u00b0C \u5132\u5b58\uff0c\u6709\u6548\u671f 12 \u500b\u6708\u767c\u8ca8\u5730\uff1a\u4e0a\u6d77\uff0c\u4e2d\u570b\u50c5\u4f9b\u7814\u7a76\u4f7f\u7528\u79bd\u6d41\u611f\u6aa2\u6e2c\u8a66\u5291\u76d2\u6982\u8ff0 \u672c\u79bd\u6d41\u611f\u6aa2\u6e2c\u8a66\u5291\u76d2\u5c08\u9580\u8a2d\u8a08\u7528\u65bc\u5b9a\u6027\u548c\u5b9a\u91cf\u6aa2\u6e2c\u7532\u578b\u6d41\u611f\u75c5\u6bd2 H1N1 \u4e9e\u578b RNA...<\/p>","protected":false},"featured_media":408,"comment_status":"open","ping_status":"closed","template":"","meta":{"_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"default","_kad_post_title":"","_kad_post_layout":"default","_kad_post_sidebar_id":"","_kad_post_content_style":"default","_kad_post_vertical_padding":"default","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"_kad_post_classname":""},"product_brand":[126,19],"product_cat":[125],"product_tag":[],"class_list":["post-407","product","type-product","status-publish","has-post-thumbnail","product_brand-sanshi-biotech","product_brand-seno","product_cat-bird-dna-test","first","instock","shipping-taxable","product-type-simple","entry","content-bg","loop-entry"],"_links":{"self":[{"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/product\/407","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/comments?post=407"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/media\/408"}],"wp:attachment":[{"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/media?parent=407"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/product_brand?post=407"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/product_cat?post=407"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/product_tag?post=407"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}