{"id":410,"date":"2025-07-01T09:16:36","date_gmt":"2025-07-01T09:16:36","guid":{"rendered":"https:\/\/birdstesting.com\/?post_type=product&p=410"},"modified":"2025-07-01T09:17:17","modified_gmt":"2025-07-01T09:17:17","slug":"%e9%b4%bf%e5%89%af%e9%bb%8f%e6%b6%b2%e7%97%85%e6%af%92%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92","status":"publish","type":"product","link":"https:\/\/birdstesting.com\/zh\/%e9%b4%bf%e5%89%af%e9%bb%8f%e6%b6%b2%e7%97%85%e6%af%92%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92\/","title":{"rendered":"\u9d3f\u526f\u9ecf\u6db2\u75c5\u6bd2 1 \u578b (PPMV-1) RNA \u6e2c\u8a66\u5957\u4ef6 - RT-qPCR"},"content":{"rendered":"

\u76ee\u9304\u7de8\u865f\uff1a<\/strong> AP2409006\u898f\u683c\uff1a<\/strong> 50T \/ 100T\u5132\u5b58\uff1a<\/strong> -20\u00b0C<\/p>\n

\n

\u7522\u54c1\u7e3d\u89bd\uff1a<\/strong><\/h3>\n

\u7684 \u9d3f\u526f\u9ecf\u6db2\u75c5\u6bd2 1 \u578b (PPMV-1) RNA \u6aa2\u6e2c\u8a66\u5291\u76d2<\/strong> \u662f\u4e00\u7a2e\u9ad8\u9748\u654f\u5ea6\u7684 RT-qPCR \u8a3a\u65b7\u5de5\u5177\uff0c\u7528\u65bc\u8b58\u5225 PPMV-1<\/strong>,......\u3002 RNA \u75c5\u6bd2<\/strong> \u5c0e\u81f4 \u65b0\u57ce\u75c5<\/em> \u9d3f\u5b50\u3002\u53d7\u611f\u67d3\u9d3f\u5b50\u7684\u81e8\u5e8a\u75c7\u72c0\u5305\u62ec \u8179\u7009\u3001\u817f\u90e8\u9ebb\u75fa\u3001\u986b\u6296\u548c\u795e\u7d93\u75c7\u72c0<\/strong>.<\/p>\n

PPMV-1 \u662f \u9ad8\u5ea6\u4f20\u67d3\u6027<\/strong>\u816b\u7624\uff0c\u767c\u75c5\u7387\u548c\u6b7b\u4ea1\u7387\u90fd\u5f88\u9ad8\u3002\u5b83\u900f\u904e\u4ee5\u4e0b\u9014\u5f91\u8fc5\u901f\u50b3\u64ad \u53e3\u8154\u3001\u547c\u5438\u9053\u3001\u773c\u90e8\u3001\u751f\u6b96\uff0c\u751a\u81f3\u662f\u8207\u50b7\u53e3\u6709\u95dc\u7684<\/strong> \u8def\u7dda\u3002\u53ca\u6642\u4e14\u6e96\u78ba\u7684\u6aa2\u6e2c\u5c0d\u65bc\u75be\u75c5\u63a7\u5236\u3001\u9810\u9632\u4e8c\u6b21\u611f\u67d3\u53ca\u904f\u5236\u75ab\u60c5\u7206\u767c\u81f3\u95dc\u91cd\u8981\u3002<\/p>\n

\u672c\u8a66\u5291\u76d2\u4ee5 PPMV-1 \u7684\u4fdd\u5b88\u57fa\u56e0\u5e8f\u5217\u70ba\u57fa\u790e\uff0c\u4e26\u91dd\u5c0d\u4ee5\u4e0b\u7528\u9014\u9032\u884c\u4e86\u512a\u5316 \u5728\u53e3\u8154\/\u53e3\u8154\u62ed\u5b50\u3001\u8840\u6db2\u548c\u7d44\u7e54\u4e2d\u5feb\u901f\u6aa2\u6e2c<\/strong> \u9d3f\u5b50\u548c\u5176\u4ed6\u79bd\u9ce5\u54c1\u7a2e\u901a\u904e \u4e00\u6b65\u9006\u8f49\u9304\u5b9a\u91cf PCR<\/a> (RT-qPCR)<\/strong>.<\/p>\n

\n

\u4e3b\u8981\u529f\u80fd\uff1a<\/strong><\/h3>\n
    \n
  • \n

    \u4e00\u6b65\u5f0f RT-qPCR<\/strong> \u7528\u65bc\u76f4\u63a5 RNA \u6aa2\u6e2c<\/p>\n<\/li>\n

  • \n

    \u9069\u7528\u65bc \u62ed\u5b50\u3001\u8840\u6db2 (EDTA)\u3001\u7d44\u7e54\u5747\u8cea\u7269<\/strong><\/p>\n<\/li>\n

  • \n

    \u5305\u62ec \u967d\u6027\u548c\u9670\u6027\u5c0d\u7167<\/strong><\/p>\n<\/li>\n

  • \n

    \u5feb\u901f\u53d6\u5f97\u6210\u679c\u3001 \u9ad8\u9748\u654f\u5ea6\u548c\u7279\u7570\u6027<\/strong><\/p>\n<\/li>\n

  • \n

    \u4fdd\u5b58\u671f\u9650\u9577\uff1a-20\u00b0C \u4e0b\u226512 \u500b\u6708<\/p>\n<\/li>\n

  • \n

    \u662f\u9d3f\u5b50\u98fc\u990a\u8005\u3001\u9ce5\u985e\u5be6\u9a57\u5ba4\u548c\u7378\u91ab\u76e3\u63a7\u7684\u7406\u60f3\u9078\u64c7<\/p>\n<\/li>\n<\/ul>\n

    \n

    \u5957\u4ef6\u7d44\u4ef6\uff1a<\/strong><\/h3>\n
    \n
    \n\n\n\n\n\n\n\n\n
    \u7d44\u4ef6<\/th>\n50T<\/th>\n100T<\/th>\n<\/tr>\n<\/thead>\n
    PPMV-1 \u53cd\u61c9\u7de9\u885d\u6db2<\/td>\n575 \u00b5L<\/td>\n1150 \u00b5L<\/td>\n<\/tr>\n
    PPMV-1 \u9810\u6df7\u5408\u6599<\/td>\n575 \u00b5L<\/td>\n1150 \u00b5L<\/td>\n<\/tr>\n
    PPMV-1 \u967d\u6027\u5c0d\u7167<\/td>\n100 \u00b5L<\/td>\n100 \u00b5L<\/td>\n<\/tr>\n
    \u9670\u6027\u5c0d\u7167<\/td>\n100 \u00b5L<\/td>\n100 \u00b5L<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
    \n
    <\/div>\n<\/div>\n<\/div>\n<\/div>\n
    \n

    \u6a23\u54c1\u985e\u578b\u8207\u8981\u6c42\uff1a<\/strong><\/h3>\n
      \n
    • \n

      \u53e3\u8154\/\u53e3\u8154\u62ed\u5b50<\/strong>:\u7121\u83cc\u62ed\u5b50\u5132\u5b58\u65bc 2-8\u00b0C\uff0824 \u5c0f\u6642\u5167\uff09\u6216 -20\u00b0C<\/p>\n<\/li>\n

    • \n

      \u5168\u8840<\/strong>:\u50c5\u4f9b\u4f7f\u7528 EDTA \u6297\u51dd\u5291<\/strong>\u907f\u514d\u4f7f\u7528\u809d\u7d20\uff1b\u5728 2-8\u00b0C \u5132\u5b58 \u22647 \u5929\u6216\u5728 -20\u00b0C \u5132\u5b58 \u22643 \u500b\u6708<\/p>\n<\/li>\n

    • \n

      \u7d44\u7e54<\/strong>\uff1a \u2264100 \u6beb\u514b\u65b0\u9bae\u808c\u8089\u6216\u5668\u5b98\u7d44\u7e54\uff0c\u5728 200-500 \u5fae\u5347\u7121\u83cc\u6c34\u4e2d\u5747\u8cea<\/p>\n<\/li>\n<\/ul>\n

      \u61c9\u4f7f\u7528 MD023 \u5b89\u5168 RNA \u62bd\u63d0\u5957\u4ef6<\/strong> \u6216\u7b49\u6548\u7269\u3002\u8403\u53d6\u7684 RNA \u61c9\u5132\u5b58\u65bc 4\u00b0C (\u22647\u5929) \u6216 -20\u00b0C (\u22646\u500b\u6708)\u3002<\/p>\n

      \n

      RT-qPCR \u53cd\u61c9\u8a2d\u5b9a\uff1a<\/strong><\/h3>\n
        \n
      1. \n

        \u89e3\u51cd\u8a66\u5291\u4e26\u7c21\u55ae\u96e2\u5fc3\u3002<\/p>\n<\/li>\n

      2. \n

        \u9069\u7528\u65bc n \u500b\u6a23\u672c + 2 \u500b\u5c0d\u7167<\/strong>, \u6df7\u5408\uff1a<\/p>\n

          \n
        • \n

          (n+2) \u00d7 11.5 \u00b5L PPMV-1 \u53cd\u61c9\u7de9\u885d\u6db2<\/strong><\/p>\n<\/li>\n

        • \n

          (n+2) \u00d7 11.5 \u00b5L PPMV-1 \u9810\u6df7\u5408\u6599<\/strong><\/p>\n<\/li>\n<\/ul>\n<\/li>\n

        • \n

          \u652a\u62cc\u5747\u52fb\u5f8c\u5206\u914d 23 \u00b5L<\/strong> \u6bcf\u500b PCR \u7ba1<\/p>\n<\/li>\n

        • \n

          \u65b0\u589e 2 \u00b5L \u6a23\u54c1 RNA<\/strong>, \u6b63\u9762<\/strong>\u6216 \u9670\u6027\u5c0d\u7167<\/strong><\/p>\n<\/li>\n

        • \n

          \u6700\u7d42\u53cd\u61c9\u9ad4\u7a4d\uff1a 25 \u00b5L<\/strong><\/p>\n<\/li>\n<\/ol>\n

          \n

          PCR \u5faa\u74b0\u689d\u4ef6\uff1a<\/strong><\/h3>\n
          \n
          \n\n\n\n\n\n\n\n\n
          \u6b65\u9a5f<\/th>\n\u6eab\u5ea6<\/th>\n\u6642\u9593<\/th>\n<\/tr>\n<\/thead>\n
          \u53cd\u5411\u8f49\u9304<\/td>\n50\u00b0C<\/td>\n10 \u5206\u9418<\/td>\n<\/tr>\n
          \u9810\u8b8a\u6027<\/td>\n95\u00b0C<\/td>\n2 \u5206\u9418<\/td>\n<\/tr>\n
          PCR \u64f4\u589e (\u00d740)<\/td>\n95\u00b0C<\/td>\n5 \u79d2<\/td>\n<\/tr>\n
          <\/td>\n59\u00b0C<\/td>\n35 \u79d2 (FAM \u901a\u9053)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
          \n
          <\/div>\n<\/div>\n<\/div>\n<\/div>\n
          \n

          \u8207 ABI \u53ca\u5176\u4ed6\u5373\u6642 PCR \u5e73\u53f0\u76f8\u5bb9\u3002ROX \u67d3\u6599\u53ef\u9078\u3002<\/p>\n<\/blockquote>\n

          \n

          \u7d50\u679c\u95e1\u91cb\uff1a<\/strong><\/h3>\n
            \n
          • \n

            \u6b63\u9762<\/strong>:Ct \u2264 33\uff0c\u5177\u6709\u6e05\u6670\u7684 \"S \"\u66f2\u7dda<\/p>\n<\/li>\n

          • \n

            \u61f7\u7591<\/strong>:33 < Ct < 38 \u2192 \u5efa\u8b70\u91cd\u65b0\u6e2c\u8a66<\/p>\n<\/li>\n

          • \n

            \u9670\u6027<\/strong>:Ct \u2265 38 \u6216\u7121\u64f4\u589e\u66f2\u7dda<\/p>\n<\/li>\n<\/ul>\n

            \u63a7\u5236\u8981\u6c42<\/strong>:<\/p>\n

              \n
            • \n

              \u967d\u6027\u5c0d\u7167\uff1aCt < 28<\/p>\n<\/li>\n

            • \n

              \u9670\u6027\u5c0d\u7167\uff1aCt \u2265 38 \u6216\u7121 Ct \u503c<\/p>\n<\/li>\n<\/ul>\n

              \n

              \u9810\u9632\u63aa\u65bd\uff1a<\/strong><\/h3>\n
                \n
              1. \n

                \u5728\u7368\u7acb\u7684\u5de5\u4f5c\u5340\u57f7\u884c\uff1aRNA \u9810\u5099\u3001\u8a66\u5291\u8a2d\u5b9a\u548c PCR \u64f4\u589e<\/p>\n<\/li>\n

              2. \n

                \u6234\u624b\u5957\u3001\u7a7f\u767d\u8902\uff0c\u4e26\u5728\u6bcf\u500b\u5340\u57df\u4f7f\u7528\u7368\u7acb\u7684\u79fb\u6db2\u5668\u548c\u8a2d\u5099<\/p>\n<\/li>\n

              3. \n

                \u907f\u514d\u91cd\u8907\u51b7\u51cd-\u878d\u5316\u5faa\u74b0\uff1b\u9632\u6b62\u8a66\u5291\u898b\u5149<\/p>\n<\/li>\n

              4. \n

                \u50c5\u4f9b\u7814\u7a76\u4f7f\u7528\uff1b\u4e0d\u64ec\u7528\u65bc\u81e8\u5e8a\u8a3a\u65b7<\/p>\n<\/li>\n

              5. \n

                \u5b9a\u671f\u4f7f\u7528\u9152\u7cbe\u3001\u6c2f\u6c23\u6216\u7d2b\u5916\u7dda\u6d88\u6bd2\u5de5\u4f5c\u5340\u548c\u8a2d\u5099<\/p>\n<\/li>\n<\/ol>\n

                \n

                \ud83d\uded2 \u53ca\u65e9\u6709\u6548\u5730\u6aa2\u6e2c PPMV-1\u3002<\/strong>
                \u662f\u98fc\u990a\u8005\u3001\u8a3a\u65b7\u5be6\u9a57\u5ba4\u548c\u79bd\u75c5\u7814\u7a76\u4eba\u54e1\u4fdd\u969c\u9d3f\u7fa4\u5065\u5eb7\u548c\u964d\u4f4e\u9d3f\u526f\u9ecf\u6db2\u75c5\u6bd2\u7206\u767c\u9020\u6210\u6b7b\u4ea1\u98a8\u96aa\u7684\u91cd\u8981\u5de5\u5177\u3002<\/p>","protected":false},"excerpt":{"rendered":"

                \u76ee\u9304\u7de8\u865f: AP2409006 \u898f\u683c\uff1a50T \/ 100T \u5132\u5b58\u6eab\u5ea6\uff1a-20\u00b0C \u7522\u54c1\u6982\u8ff0\uff1a\u9d3f\u526f\u9ecf\u6db2\u75c5\u6bd2 1 \u578b (PPMV-1) RNA \u6aa2\u6e2c\u8a66\u5291\u76d2\u662f\u4e00\u7a2e\u9ad8\u9748\u654f\u5ea6\u7684 RT-qPCR \u8a3a\u65b7\u5de5\u5177\uff0c\u7528\u65bc\u9451\u5b9a PPMV-1\uff0cPPMV-1 \u662f\u4e00\u7a2e RNA \u75c5\u6bd2\uff0c\u6703\u5f15\u8d77\u9d3f\u5b50\u65b0\u57ce\u75ab\u3002\u53d7\u611f\u67d3\u9d3f\u5b50\u7684\u81e8\u5e8a\u75c7\u72c0\u5305\u62ec\u8179\u7009\u3001\u817f\u90e8\u9ebb\u75fa\u3001\u9707\u986b\u548c\u795e\u7d93\u75c7\u72c0\u3002PPMV-1 \u5177\u6709\u9ad8\u5ea6\u50b3\u67d3\u6027\uff0c...<\/p>","protected":false},"featured_media":411,"comment_status":"open","ping_status":"closed","template":"","meta":{"_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"default","_kad_post_title":"","_kad_post_layout":"default","_kad_post_sidebar_id":"","_kad_post_content_style":"default","_kad_post_vertical_padding":"default","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"_kad_post_classname":""},"product_brand":[126],"product_cat":[125],"product_tag":[],"class_list":["post-410","product","type-product","status-publish","has-post-thumbnail","product_brand-sanshi-biotech","product_cat-bird-dna-test","first","instock","shipping-taxable","product-type-simple","entry","content-bg","loop-entry"],"_links":{"self":[{"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/product\/410","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/comments?post=410"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/media\/411"}],"wp:attachment":[{"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/media?parent=410"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/product_brand?post=410"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/product_cat?post=410"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/birdstesting.com\/zh\/wp-json\/wp\/v2\/product_tag?post=410"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}