Kit de prueba de ADN del herpesvirus de las palomas (PiHV) - Método de la sonda fluorescente PCR (50T / 100T)

Pigeon Herpesvirus (PiHV) DNA Test Kit

This avian Prueba de ADN kit is designed to detect Pigeon Herpesvirus Type I (PiHV1), a major cause of young pigeon disease syndrome. PiHV infections typically affect young pigeons and are characterized by rhinitis, conjunctivitis, lethargy, loss of appetite, diarrhea, vomiting, and neurological symptoms.

Using a highly optimized PCR-fluorescent probe method, this kit offers fast and accurate detection of PiHV DNA in bird oral/cloacal swabs, blood, and tissue samples. Early detection helps prevent disease outbreaks, reduce mortality, and support effective treatment strategies.

Características principales:

✅ Detects Pigeon Herpesvirus (PiHV1) with high specificity and sensitivity
✅ Sample compatibility: oral/cloacal swabs, EDTA blood, liver tissue
✅ Includes positive and negative controls for each run
✅ Easy PCR workflow with minimal hands-on time
✅ Ideal for use in pigeon lofts, vet labs, and disease monitoring programs
✅ Shelf life: ≥12 months at -20°C

Suitable For:

• Racing pigeons

• Fancy pigeons

• Young pigeons in breeding programs

• All PiHV-susceptible avian species

Kit Components:

PCR Protocol:

• Reaction volume: 25 μL (23 μL reagent + 2 μL sample)

• Thermal cycling:
  • 50°C for 2 min (UNG)
  • 95°C for 5 min
  • 40 cycles of:
     95°C for 10 sec
     60°C for 35 sec (fluorescence collection)

• Detection Channel: FAM

• Compatible with ABI and most real-time PCR instruments

Interpretación de los resultados:

• Positivo: Ct ≤ 33 and S-curve present

• Sospechoso: 33 < Ct < 38 – retesting recommended after ruling out inhibitors or contamination

• Negativo: No Ct or Ct ≥ 38 with no S-curve

Positive and negative controls must meet QC criteria for results to be valid.

Storage & Handling:

• Store at -20°C

• Avoid repeated freeze-thaw cycles

• Protect reagents from light

• For research use only – not for clinical diagnostics

Recommended Use With:

• DNA Extraction Kit (e.g., DP202)

• PPE and aerosol contamination control

• Other bird virus PCR kits (PBFD, Polyomavirus, Chlamydia psittaci, etc.)

• Género de las aves test kits for full avian DNA profiling

Protect your pigeons with accurate viral DNA testing.
Add this PiHV DNA test kit to your avian health screening toolbox.

🛒 Order now and ensure early detection of pigeon herpesvirus!