Total DNA/RNA Extraction Kit (Spin Column Method) For Birds Virus Test
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Catalog No.: DP201-01 / DP201-02
Specifications: 25T / 50T
Storage: Room Temperature (15–25°C)
Product Description
The Total DNA/RNA Extraction Kit is designed for rapid and efficient purification of high-quality total nucleic acids—including viral, bacterial, and parasitic DNA/RNA—from a variety of biological samples such as tissues, blood, secretions, and excreta.
Utilizing silica membrane spin column technology, the kit enables reliable nucleic acid extraction without the need for toxic phenol-chloroform or time-consuming alcohol precipitation. The purified nucleic acids are suitable for a wide range of downstream applications, including PCR, RT-PCR, LAMP/RT-LAMP, and Southern/Northern blotting.
Principle of the Method
Under high-salt conditions, nucleic acids bind selectively to the silica membrane in the spin column, while proteins and impurities are washed away. After multiple wash steps, high-purity DNA/RNA is eluted using DEPC-treated water, ready for immediate use in molecular biology workflows.
Kit Contents
Component | DP201-01 (25T) | DP201-02 (50T) | Storage |
---|---|---|---|
Lysis Buffer | 15 mL | 30 mL | RT |
Wash Buffer | 5 mL | 10 mL | RT |
Elution Buffer | 5 mL | 10 mL | RT |
Spin Columns (Tube A) | 25 pcs | 50 pcs | RT |
Collection Tubes (B) | 25 pcs | 50 pcs | RT |
⚠️ Note: Add 20 mL (for 25T) or 40 mL (for 50T) of absolute ethanol to the Wash Buffer before first use.
Sample Types & Preparation
Blood: Centrifuge >500 µL EDTA-anticoagulated blood, transfer 200 µL of plasma for extraction.
Tissue: Homogenize 0.05–1 g tissue in saline (5–10× volume), take 200 µL homogenate for processing.
Swab/Fecal Samples: Swab the collection area after moistening in saline. Detach the swab tip into a 1 mL saline tube, vortex 30 sec, then transfer 200 µL.
Simplified Protocol of The Extraction Kit
Mix 200 µL sample with 500 µL lysis buffer → incubate 5 min → centrifuge.
Transfer 400 µL supernatant + 200 µL ethanol → vortex.
Load onto Spin Column A (in Collection Tube B) → centrifuge 1 min.
Discard flowthrough → add 600 µL wash buffer → centrifuge 1 min.
Repeat centrifugation to dry column → transfer Column A to a new tube.
Add 50 µL elution buffer → incubate 1–2 min → centrifuge → collect nucleic acids.
⏱️ Total time: ~20–30 minutes
🧊 Storage of purified nucleic acids: Store at -80°C if not used immediately.
Precautions
For single use only; do not reuse spin columns or collection tubes.
Wear gloves, a lab coat, and a mask during the operation. Avoid touching tube rims and column openings.
Disinfect work surfaces and equipment after use with 1% sodium hypochlorite or 75% ethanol.
Dispose of biological waste in accordance with safety regulations.
Applications of This Total DNA/RNA Extraction Kit
Viral and bacterial nucleic acid extraction
Avian diagnostic sample preparation
PCR, RT-PCR, and isothermal amplification
Veterinary diagnostics and research labs
Ordering Information
Product Code | Format | Sample Capacity | Storage |
---|---|---|---|
DP201-01 | 25 Tests | For small to mid-scale use | Room Temperature |
DP201-02 | 50 Tests | For high-throughput labs | Room Temperature |
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