鳥インフルエンザ検査キット（H1N1亜型ウイルス）リアルタイムPCR検出キット

Product Name (EN): Avian Influenza テストキット
Package Size: 50 tests
Price: Contact Our Sales
ストレージ： Transport at low temperature; store at -20°C, valid for 12 months
Shipping From: Shanghai, China
研究用

Avian Influenza Test Kit Overview

This Avian Influenza Test Kit is specifically designed for the qualitative and quantitative detection of Influenza A virus H1N1 subtype RNA using probe-based fluorescent qRT-PCR technology. By targeting conserved genetic regions of the H1N1 subtype, the kit delivers high specificity and sensitivity in detecting avian influenza A virus from biological and environmental samples.

Unlike SYBR Green dye-based PCR, this TaqMan probe-based system uses fluorescence-labeled probes that emit signals only upon hybridization and cleavage during amplification, ensuring superior specificity and lower false-positive rates.

主な特徴

✅ Highly Specific & Sensitive – Designed based on highly conserved regions of the H1N1 genome; no cross-reaction with non-target viruses
✅ Ready-to-Use System – Requires only RNA template from the user
✅ Optimized Primers & Probes – Ensures efficient amplification and detection
✅ Positive Control Included – Synthetic non-infectious DNA for result validation
✅ Quantitative & Qualitative Applications – Suitable for research, surveillance, and screening use
✅ Compatible with Major qPCR Instruments – Fluorescence detection on FAM channel

Kit Contents (50 tests)

仕組み

The kit detects viral RNA through a one-step reverse transcription PCR reaction. During amplification, the Taq polymerase cleaves the dual-labeled probe, separating the fluorophore from the quencher, which results in a fluorescence signal proportional to the amount of target RNA present.

Fluorescence is measured during each cycle, allowing real-time monitoring of the PCR process.

Principle of Detection

• Ct Value (Cycle Threshold): The number of PCR cycles required for fluorescence to exceed the threshold. Lower Ct indicates a higher initial target quantity.

• Threshold Setting: Based on 10× standard deviation of fluorescence from the initial 3–15 cycles (background).

Performance & Cut-Off Criteria

Positive and negative controls must pass quality checks. Ct of negative control ≥40 and positive control Ct ≤30 with exponential amplification.

Reaction Protocol

Reverse Transcription + PCR (20 μL per reaction)

• Total Volume: 20 μL

• Thermal Cycling Conditions:

Quantification (Optional)

Prepare a standard curve using serial 10-fold dilutions (10²–10⁷ copies/μL) of the synthetic DNA control. Plot Ct values against log(concentration) to interpolate RNA copy numbers in unknown samples.

Advantages of Probe-Based PCR Over SYBR Green:

Intended Use

この製品は for research purposes only. It is not approved for clinical diagnostics or therapeutic procedures.