鸚鵡喙與羽毛疾病病毒核酸測試 PBFDV 套件

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(Psittacine Beak and Feather Disease Virus Detection)
Catalog Numbers: AP2304002-01 (24 Tests) / AP2304002-02 (48 Tests)

Product Overview

This molecular diagnostic kit is specially developed for the detection of Psittacine Beak and Feather Disease Virus (PBFDV) in a wide range of avian sample types, including feathers, blood, tissues, oral swabs, and cloacal swabs. The kit utilizes real-time fluorescence PCR (qPCR) technology to amplify and detect the highly conserved gene region of PBFDV, ensuring high specificity and sensitivity.

Incorporated with an endogenous internal reference gene, the kit monitors both the nucleic acid extraction process and PCR amplification quality, effectively minimizing false negatives and improving the overall reliability of results.

主要功能

  • High Sensitivity & Specificity
    Detects conserved PBFDV gene regions with real-time PCR.

  • Built-in Internal Control
    Ensures sample quality and extraction integrity.

  • Multi-Sample Compatibility
    Applicable to feathers, EDTA-preserved blood, soft tissues, and swabs.

  • Fast & Accurate Results
    Results in 90–120 minutes with clear Ct value interpretation.


Kit Components

組件24 Tests (AP2304002-01)48 Tests (AP2304002-02)
PBFDV Reaction Solution23μL/well × 8 wells/strip × 3 strips23μL/well × 8 wells/strip × 6 strips
PBFDV Positive Control100μL100μL
陰性對照100μL100μL

儲存: Store at -20°C; shelf life ≥ 12 months.


Sample Requirements

  • 羽毛: At least 5 fresh feathers (with shaft) from chest, abdomen, or leg. Do 不是 use naturally shed feathers.

  • 血液: EDTA-preserved; store at 2–8°C (≤7 days) or -20°C (≤3 months). Avoid heparin and repeated freeze-thaw cycles.

  • 組織: ≤100mg soft organ or muscle tissue (no skin/fascia); homogenize in sterile water.

  • DNA 萃取: Use a validated kit such as Three Lions Biotech Animal Genomic DNA Rapid Extraction Kit (DP202).


Workflow Summary

  1. Sample Processing

    • Extract nucleic acids per standard kit protocols.

    • Store DNA on ice during preparation and testing.

  2. Reaction Setup

    • Thaw all reagents, centrifuge briefly.

    • Add 2μL of sample DNA, positive or negative control, into 23μL reaction mix (final volume: 25μL).

    • Mix and centrifuge before loading onto the PCR machine.

  3. PCR 擴增

    • Pre-denaturation: 95°C for 3 min

    • 35 Cycles of:

      • Denaturation: 95°C for 10 sec

      • Annealing/Extension: 60°C for 40 sec

      • Fluorescence detection at 60°C

    • Channels: FAM (PBFDV target), VIC/HEX (internal control)

  4. Result Interpretation

    • Valid test: Positive control Ct < 30 with S-curve; negative control Ct ≥ 35 or no curve.

    • Internal control (VIC): Ct ≤ 32 = valid sample.

    • PBFDV detection (FAM):

      • Ct ≤ 32: Positive

      • 32 < Ct < 35: Suspect – retest

      • Ct ≥ 35 or no curve: Negative


預防措施

  • Conduct all steps in designated clean areas to prevent cross-contamination.

  • Wear gloves and lab coats; use separate pipettes for pre- and post-PCR.

  • Do not open PCR tubes immediately after reaction; allow them to cool.

  • Reagents are for research use only. Not intended for clinical diagnosis.


應用

  • Rapid detection of PBFDV in parrots and other psittacine birds

  • Suitable for bird breeders, veterinarians, pet labs, and avian disease researchers

  • Compatible with most mainstream qPCR systems (ABI, Bio-Rad, Roche, etc.)


訂購資訊

產品編號格式Test Capacity儲存
AP2304002-0124T Kit24 reactions-20°C
AP2304002-0248T Kit48 reactions-20°C

Contact Us for Bulk Orders or OEM Services

Need customized packaging, veterinary certification support, or a large-volume purchase? Contact our technical sales team for assistance.

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搶先評價 “Parrot Beak and Feather Disease Virus Nucleic Acid Test PBFDV Kit”

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