(Psittacine Beak and Feather Disease Virus Detection)
Catalog Numbers: AP2304002-01 (24 Tests) / AP2304002-02 (48 Tests)
Product Overview
This molecular diagnostic kit is specially developed for the detection of Psittacine Beak and Feather Disease Virus (PBFDV) in a wide range of avian sample types, including feathers, blood, tissues, oral swabs, and cloacal swabs. The kit utilizes real-time fluorescence PCR (qPCR) technology to amplify and detect the highly conserved gene region of PBFDV, ensuring high specificity and sensitivity.
Incorporated with an endogenous internal reference gene, the kit monitors both the nucleic acid extraction process and PCR amplification quality, effectively minimizing false negatives and improving the overall reliability of results.
主要功能
✅ High Sensitivity & Specificity
Detects conserved PBFDV gene regions with real-time PCR.✅ Built-in Internal Control
Ensures sample quality and extraction integrity.✅ Multi-Sample Compatibility
Applicable to feathers, EDTA-preserved blood, soft tissues, and swabs.✅ Fast & Accurate Results
Results in 90–120 minutes with clear Ct value interpretation.
Kit Components
組件 | 24 Tests (AP2304002-01) | 48 Tests (AP2304002-02) |
---|---|---|
PBFDV Reaction Solution | 23μL/well × 8 wells/strip × 3 strips | 23μL/well × 8 wells/strip × 6 strips |
PBFDV Positive Control | 100μL | 100μL |
陰性對照 | 100μL | 100μL |
儲存: Store at -20°C; shelf life ≥ 12 months.
Sample Requirements
羽毛: At least 5 fresh feathers (with shaft) from chest, abdomen, or leg. Do 不是 use naturally shed feathers.
血液: EDTA-preserved; store at 2–8°C (≤7 days) or -20°C (≤3 months). Avoid heparin and repeated freeze-thaw cycles.
組織: ≤100mg soft organ or muscle tissue (no skin/fascia); homogenize in sterile water.
DNA 萃取: Use a validated kit such as Three Lions Biotech Animal Genomic DNA Rapid Extraction Kit (DP202).
Workflow Summary
Sample Processing
Extract nucleic acids per standard kit protocols.
Store DNA on ice during preparation and testing.
Reaction Setup
Thaw all reagents, centrifuge briefly.
Add 2μL of sample DNA, positive or negative control, into 23μL reaction mix (final volume: 25μL).
Mix and centrifuge before loading onto the PCR machine.
PCR 擴增
Pre-denaturation: 95°C for 3 min
35 Cycles of:
Denaturation: 95°C for 10 sec
Annealing/Extension: 60°C for 40 sec
Fluorescence detection at 60°C
Channels: FAM (PBFDV target), VIC/HEX (internal control)
Result Interpretation
Valid test: Positive control Ct < 30 with S-curve; negative control Ct ≥ 35 or no curve.
Internal control (VIC): Ct ≤ 32 = valid sample.
PBFDV detection (FAM):
Ct ≤ 32: Positive
32 < Ct < 35: Suspect – retest
Ct ≥ 35 or no curve: Negative
預防措施
Conduct all steps in designated clean areas to prevent cross-contamination.
Wear gloves and lab coats; use separate pipettes for pre- and post-PCR.
Do not open PCR tubes immediately after reaction; allow them to cool.
Reagents are for research use only. Not intended for clinical diagnosis.
應用
Rapid detection of PBFDV in parrots and other psittacine birds
Suitable for bird breeders, veterinarians, pet labs, and avian disease researchers
Compatible with most mainstream qPCR systems (ABI, Bio-Rad, Roche, etc.)
訂購資訊
產品編號 | 格式 | Test Capacity | 儲存 |
---|---|---|---|
AP2304002-01 | 24T Kit | 24 reactions | -20°C |
AP2304002-02 | 48T Kit | 48 reactions | -20°C |
Contact Us for Bulk Orders or OEM Services
Need customized packaging, veterinary certification support, or a large-volume purchase? Contact our technical sales team for assistance.
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