Kit de extração de DNA/RNA total (método de coluna de rotação) para teste de vírus de aves

Catalog No.: DP201-01 / DP201-02
Specifications: 25T / 50T
Storage: Room Temperature (15–25°C)

Product Description

The Total DNA/RNA Extraction Kit is designed for rapid and efficient purification of high-quality total nucleic acids—including viral, bacterial, and parasitic DNA/RNA—from a variety of biological samples such as tissues, blood, secretions, and excreta.

Utilizing silica membrane spin column technology, the kit enables reliable nucleic acid extraction without the need for toxic phenol-chloroform or time-consuming alcohol precipitation. The purified nucleic acids are suitable for a wide range of downstream applications, including PCR, RT-PCR, LAMP/RT-LAMP, and Southern/Northern blotting.

Principle of the Method

Under high-salt conditions, nucleic acids bind selectively to the silica membrane in the spin column, while proteins and impurities are washed away. After multiple wash steps, high-purity DNA/RNA is eluted using DEPC-treated water, ready for immediate use in molecular biology workflows.

Kit Contents

⚠️ Note: Add 20 mL (for 25T) ou 40 mL (for 50T) of absolute ethanol to the Wash Buffer before first use.

Sample Types & Preparation

• Sangue: Centrifuge >500 µL EDTA-anticoagulated blood, transfer 200 µL of plasma for extraction.

• Tecido: Homogenize 0.05–1 g tissue in saline (5–10× volume), take 200 µL homogenate for processing.

• Swab/Fecal Samples: Swab the collection area after moistening in saline. Detach the swab tip into a 1 mL saline tube, vortex 30 sec, then transfer 200 µL.

Simplified Protocol of The Extraction Kit

1. Mix 200 µL sample with 500 µL lysis buffer → incubate 5 min → centrifuge.

2. Transfer 400 µL supernatant + 200 µL ethanol → vortex.

3. Load onto Spin Column A (in Collection Tube B) → centrifuge 1 min.

4. Discard flowthrough → add 600 µL wash buffer → centrifuge 1 min.

5. Repeat centrifugation to dry column → transfer Column A to a new tube.

6. Add 50 µL elution buffer → incubate 1–2 min → centrifuge → collect nucleic acids.

⏱️ Total time: ~20–30 minutes
🧊 Storage of purified nucleic acids: Store at -80°C if not used immediately.

Precautions

• For single use only; do not reuse spin columns or collection tubes.

• Wear gloves, a lab coat, and a mask during the operation. Avoid touching tube rims and column openings.

• Disinfect work surfaces and equipment after use with 1% sodium hypochlorite or 75% ethanol.

• Dispose of biological waste in accordance with safety regulations.

Applications of This Total DNA/RNA Extraction Kit

• Viral and bacterial nucleic acid extraction

• Avian diagnostic sample preparation

• PCR, RT-PCR, and isothermal amplification

• Veterinary diagnostics and research labs

Ordering Information